Multiplex Reverse Transcription-PCR for Simultaneous Detection of
 Beet Necrotic Yellow Vein Virus
 ,
 Beet Soilborne Virus
 , and
 Beet Virus Q
 and Their Vector
 Polymyxa betae
 KESKIN on Sugar Beet

Meunier, Alexandre; Schmit, Jean-François; Stas, A.; Kutluk, Nazli; Bragard, Claude

doi:10.1128/aem.69.4.2356-2360.2003

Cited by 72 publications

(42 citation statements)

References 33 publications

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“…PCR-based methods for PVY and PLRV detection have been shown to provide a relatively fast alternative to winter grow-out and ELISA post-harvest testing. The detection threshold of PCR-based methods was up to 128 times greater than that of an ELISA (Meunier et al 2003).…”

Section: Introductionmentioning

confidence: 94%

Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber

Xie

2006

Austral. Plant Disease Notes

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Abstract. A multiplex reverse transcription polymerase chain reaction (M-RT-PCR) was developed for the simultaneous detection of three major potato viruses, Potato virus X (PVX), Potato virus S (PVS) and Potato leaf roll virus (PLRV), in potato leaves and tubers (stored and dormant). Primers were designed from the coat protein gene of all three viruses and were able to detect virus in leaf RNA dilutions of 1 : 1024 to 1 : 4096 and from tuber RNA dilutions of 1 : 256 to 1 : 1024. The sensitivity of virus detection in leaves was higher than in tubers. All three viruses were successfully detected from tissues sampled from the tuber cortex, perimedulla and pith, with all PCR bands being strongest from cortex tissues. The reliability of the M-RT-PCR to detect PVX, PVS and PLRV in tubers was determined by testing 95 samples of field, greenhouse and in vitro potato plantlets from Wuhan, China and comparing the results with enzymelinked immunosorbent assays (ELISA). M-RT-PCR detected all infections found by ELISA in leaf and stored tubers. In freshly harvested tubers, M-RT-PCR proved more sensitive than ELISA, as indicated by an additional 3.2% of infections detected with the M-RT-PCR method.

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Section: Introductionmentioning

confidence: 94%

Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber

Xie

2006

Austral. Plant Disease Notes

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“…Relationships between soil properties and soilborne viruses transmitted by P. betae 767 (Turina et al, 1996;Mouhanna et al, 2002;Meunier et al, 2003). P. betae in this study was common (89.6%) in sugar beet fields and 35.4% of the roots were infested with aviruliferous P. betae cystosori (Table 2) (Fig.…”

Section: Single Infections With Bnyvvmentioning

confidence: 99%

“…Samples were considered positive when the absorbance values at 405 nm were more than two times the mean of the negative control (Meunier et al, 2003). Also, the root samples were stained with lactophenol containing 0.1% acid fuchsin to examine P. betae cyctosori under a light microscope (Leica, Switzerland) (Abe and Tamada, 1986).…”

Section: Introductionmentioning

confidence: 99%

Short communication. Relationships between soil properties and soilborne viruses transmitted by Polymyxa betae Keskin in sugar beet fields

Yilmaz

Arlı-Sökmen

Gülser

et al. 2010

Span. j. agric. res.

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Sugar beet plants (cv. Arosa), susceptible to rhizomania, were grown in 144 soil samples taken from sugar beet fields in north and central part of Turkey in 2004. Incidences of Beet necrotic yellow vein virus (BNYVV) and Beet soilborne virus (BSBV) by ELISA and of their vector Polymyxa betae by root staining method were determined in bait plants. Some soil properties such as, texture, pH, organic matter, cation exchange capacity, electrical conductivity, lime (CaCO3) and exchangeable Ca, Mg, K and Na contents were determined. Sand content and pH values of the soils gave significant positive correlations with P. betae (0.177* and 0.164*, respectively). Lime (CaCO3) and exchangeable Mg contents had also significant positive correlations with soil pH. Increasing CaCO3 (0.189*) and exc. Mg (0.235**) content of soils induced BNYVV and BSBV infections respectively, transmitted by P. betae, due to their increasing effects on soil pH.

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“…BNYVV occurred with BSBV and often with BVQ as well. The major advantage of this technique is the simultaneous detection of three viruses, facilitating epidemiological and resistance breeding investigations to gather reliable data (Meunier et al 2003).…”

Section: ) (Appendix 3)mentioning

confidence: 99%

Detection of Viral and Viroid Plant Pathogens in the Environment

Narayanasamy¹

2010

Microbial Plant Pathogens-Detection and Disease Diagnosis:

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Plant viruses and viroids with obligate parasitism as the mode of existence, have to be in association with living cells of plant hosts always. However, some highly stable viruses have been detected in a free state in soil, water or air. Other viruses depend on the vectors such as insects, mites, nematodes or fungi present in the environment for their spread from infected plants to healthy plants which may be available in the same field or far away. The role of alternative/additional host plant species functioning as sources of virus infection has been demonstrated by applying various diagnostic techniques. The presence of viruses has been detected by employing biological, immunological and nucleic acid-based techniques. The effectiveness and reliability of detection varies significantly. However, molecular techniques have been shown to be more sensitive, specific and fast in providing the results, although they are unable to indicate the level of pathogenic potential of the viruses detected in the environmental samples. Viroids lacking natural vectors and stability outside living cells have been detected in several additional/alternative plant species which may function as reservoir of viroid infection.

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Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus , Beet Soilborne Virus , and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet

Cited by 72 publications

References 33 publications

Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber

Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber

Short communication. Relationships between soil properties and soilborne viruses transmitted by Polymyxa betae Keskin in sugar beet fields

Detection of Viral and Viroid Plant Pathogens in the Environment

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