Multiplex stimulated Raman imaging with white probe-light from a photonic-crystal fibre and with multi-wavelength balanced detection

Seto, Kazuto; Okuda, Yuko; Tokunaga, Eiji; Kobayashi, Takayoshi

doi:10.1088/0022-3727/47/34/345401

Cited by 23 publications

(27 citation statements)

References 33 publications

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“…Note, the present configuration is similar to our previous report [26] to observe the difference signal between the probe beam and a reference beam from the same light source of the probe. In [26], the optical signal is multiplied by the synchronous signal, and its phase deviation is feedback controlled, based on the low-frequency component in the multiplied signal.…”

Section: Composition Of Phase Sensitive Detectorsupporting

confidence: 59%

“…In the present SRS microscopy scheme, the pump and probe beams from the common light source render the synchronization of plural sources unnecessary. Furthermore, we readily conduct spectral imaging through the spectral dispersion of the white probe beam [26,27]. However, the intensity noise of the white beam with the PCF is substantially high [28][29][30][31][32], causing a serious problem when the PCFs are applied to high-speed pump-probe microscopy.…”

Section: Introductionmentioning

confidence: 99%

“…Figure 2 illustrates the optics system for an SRS microscope with phase-sensitive detection. The optics system is similar to that in our previous works [26,33]. A titanium-sapphire laser (Mira 900P, COHERENT) generates the 76.3 MHz optical pulse repetition.…”

Section: Introductionmentioning

confidence: 99%

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Noise cancellation with phase-detection technique for pump-probe measurement and application to stimulated Raman imaging

et al. 2015

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Intensity noise on a probe beam is a serious obstacle to highly sensitive and high-speed pump-probe microscopy. In this report, a reference beam of the probe is prepared and delayed. The intensity modulation by the sample is measured as the phase modulation of the superposition of detected electrical signals of the probe and reference beams, and the intensity noise is canceled. We evaluate performance of the noise cancellation using the super-continuum light from a piece of photonic crystal fiber, and find that the noise is canceled by ∼26 dB. We then apply the method to a stimulated Raman microscope. This method contributes to highly sensitive and high-speed pump-probe imaging with various light sources.

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Section: Composition Of Phase Sensitive Detectorsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

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Noise cancellation with phase-detection technique for pump-probe measurement and application to stimulated Raman imaging

et al. 2015

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“…Alternatively, one can detect the full SRG/SRL spectrum in parallel using a spectrometer but with a low readout rate which strongly limits the sensitivity [45]. Multichannel lock-in amplifiers have been employed, but only at low modulation frequencies, resulting again in low sensitivity [46]. Much better results were obtained using a multi-channel active filter, consisting of a series of electronic band-pass filters and trans-impedance amplifiers [42], although with a limited number of pixels.…”

Section: B2 Broadband Stimulated Raman Scattering Microscopymentioning

confidence: 99%

Linear and Nonlinear Spectroscopy by a Common-Path Birefringent Interferometer

Preda

Oriana

Réhault

et al. 2017

IEEE J. Select. Topics Quantum Electron.

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We introduce a passive common-path interferometer to replace Michelson interferometers in Fourier transform spectroscopy. Our device exploits birefringence to introduce a highly accurate delay between two orthogonal polarization components by continuously varying the material thickness. Thanks to its inherent delay stability and reproducibility, it can be used even for short wavelengths (down to ~200nm) without the need for any active control or position tracking. We first demonstrate its performances in linear spectroscopy, by implementing a spectrometer and a spectrophotometer. We then extend its use to nonlinear spectroscopy and, in combination with lock-in detection at MHz modulation frequencies, illustrate its application to pump-probe spectroscopy with high sensitivity (ΔT⁄T<3·10-6 in 1-s integration time) and broad spectral coverage (>500nm) and to broadband stimulated Raman scattering microscopy in the CH stretching\ud region

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“…A balanced detector is commonly used to cancel the intensity noise of the probe beam in absorption and pump-probe measurements [29,30]. In a typical setup, a part of the probe beam is separated before the sample to be detected as a reference beam.…”

Section: Improvement In Snr By the Spatially Segmented Balanced Detecmentioning

confidence: 99%

Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues

Miyazaki

Kobayahsi

2017

Photonics

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Photothermal microscopy is useful to visualize the distribution of non-fluorescence chromoproteins in biological specimens. Here, we developed a high sensitivity and high resolution photothermal microscopy with low-cost and compact laser diodes as light sources. A new detection scheme for improving signal to noise ratio more than 4-fold is presented. It is demonstrated that spatial resolution in photothermal microscopy is up to nearly twice as high as that in the conventional widefield microscopy. Furthermore, we demonstrated the ability for distinguishing or identifying biological molecules with simultaneous muti-wavelength imaging. Simultaneous photothermal and fluorescence imaging of mouse brain tissue was conducted to visualize both neurons expressing yellow fluorescent protein and endogenous non-fluorescent chromophores.

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Multiplex stimulated Raman imaging with white probe-light from a photonic-crystal fibre and with multi-wavelength balanced detection

Cited by 23 publications

References 33 publications

Noise cancellation with phase-detection technique for pump-probe measurement and application to stimulated Raman imaging

Noise cancellation with phase-detection technique for pump-probe measurement and application to stimulated Raman imaging

Linear and Nonlinear Spectroscopy by a Common-Path Birefringent Interferometer

Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues

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