Multiplexed detection of foodborne pathogens using one-pot CRISPR/Cas12a combined with recombinase aided amplification on a finger-actuated microfluidic biosensor

CRISPR/Cas12a-Responsive Hydrogels for Conjugation-Free and Universal Indicator Release in Colorimetric Detection

“…For this aim, efforts should be made to integrate the CRIs-gel platform with other upstream steps including sample preprocessing and the isothermal amplification (once necessary). There have been successful demonstrations ,− that both RPA and CRISPR/Cas12a can be performed in a single step, and the Cas12a cleavage and PTS signal output can be accomplished on a microfluidic system, indicating a possibility of the one-pot or automatic procedure. Other protein indicators with smaller sizes and higher isoelectric points may also be tested for even higher encapsulation efficiency.…”

Nanozyme-Mediated Catalytic Signal Amplification for Microfluidic Biosensing of Foodborne Bacteria

“…The microfluidic chip (70 mm × 25 mm) was fabricated by standard photolithography according to our previous studies . The structure of the chip included four sets of inlets that were connected to the serpentine mixing channel, another separate inlet, an incubation chamber, an enrichment and detection chamber, and an outlet.…”

“…Pt-PCN-224 was redispersed by 200 μL of MES buffer containing 80 μg of Ab 2 and incubated at 37 °C for 1 h after centrifugation for washing twice. The complex was mixed 29 The structure of the chip included four sets of inlets that were connected to the serpentine mixing channel, another separate inlet, an incubation chamber, an enrichment and detection chamber, and an outlet. The height of the channel was about 500 μm.…”

Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

“…It is worth noting that these techniques may address some challenges such as complex design strategies, variability among users, the requirement of an external operator, and complicated micro-valving setups often require extensive microfabrication and assembly processes. [50][51][52] Additive manufacturing (3D printing) techniques can substantially address the challenges associated with microfabrication and with microfluidic handling on a large scale. 53,54 Indeed AM can bring about outcomes comparable to traditional lithography in terms of temporal and spatial resolution, material, and mechanical properties while being cost-effective, rapid, and scalable.…”

“…It is worth noting that these techniques may address some challenges such as complex design strategies, variability among users, the requirement of an external operator, and complicated micro-valving setups often require extensive microfabrication and assembly processes. 50–52…”