2006
DOI: 10.1021/ja0613106
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Multiplexed Detection of Protein Cancer Markers with Biobarcoded Nanoparticle Probes

Abstract: We have developed the chemistry for preparing a universal probe and the appropriate nano- and microparticle labels that can be used to do highly selective multiplexed detection of three protein cancer markers at low-femtomolar concentration in buffer and serum media. The approach relies on a new multiplexed version of the biobarcode amplification method and offers new opportunities for studying multiple protein markers in a single sample. This could lead to new forms of disease diagnosis and monitoring disease… Show more

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Cited by 409 publications
(304 citation statements)
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References 22 publications
(28 reference statements)
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“…The Au-NP probes were finally resuspended in 3 mL of the assay buffer and stored at 4°C. The Au-NP probe concentration was determined by measuring the absorbance of the colloid at 520 nm (34).…”
Section: Methodsmentioning
confidence: 99%
“…The Au-NP probes were finally resuspended in 3 mL of the assay buffer and stored at 4°C. The Au-NP probe concentration was determined by measuring the absorbance of the colloid at 520 nm (34).…”
Section: Methodsmentioning
confidence: 99%
“…For example, the bio-bar-code assay of Mirkin and co-workers has state-of-the-art analytical sensitivity and selectivity for the detection of nonamplified nucleic acids and proteins (Nam et al, 2004;Stoeva et al, 2006). Using this technology, attomolar target concentrations can be detected, without the complications of amplification (e.g.…”
Section: Introductionmentioning
confidence: 99%
“…Nanotechnology-based multiplex platforms are also emerging as future diagnostic approaches for sensitive and reliable detection. One of the best examples is the 'bio-barcode' developed by Mirkin, which consists of twocomponent oligonucleotide-modified nanoparticle probes [23], which can detect zeptomolar concentrations. Such nanotechnology-based platforms thus show considerable promise for probing early cell transformations by both in vivo and ex vivo measurement [24].…”
Section: Platform Selectionmentioning
confidence: 99%