Multiplexed, linkage group-specific SNP marker sets for rapid genetic mapping and fingerprinting of sugar beet (Beta vulgaris L.)

M�hring, Silke; Salamini, F.; Schneider, Katharina

doi:10.1007/s11032-004-0900-4

Cited by 30 publications

(12 citation statements)

References 29 publications

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“…Cleaved Amplified Polymorphic Sequence (CAPS) markers were developed as follows. PCR products were generated using primer sets, as described by Möhring et al (2005), Hunger et al (2003), and Schneider et al (1999), then digested with one of thirteen restriction endonucleases: Hae III, Hha I, Taq I, HapII, Mbo I, AfaI, XspI, AluI, and AccII (Takara Bio, Ohtsu, Japan); TspEI (TOYOBO, Osaka, Japan); and Mse I, HpyCH4IV, and NlaIII (New England BioLabs, Beverly, MA, USA). The resultant fragments were electrophoresed in 2% agarose gels to check for polymorphism.…”

Section: Methodsmentioning

confidence: 99%

“…Seventy-nine selected AFLP markers, whose map positions had been examined using an F 2 population derived from a cross between ‘NK-310mm-O’ and ‘NK-184mm-O’ (Taguchi et al 2009), were deemed to effectively cover the nine linkage groups of sugar beet. For CAPS markers, PCR primers were prepared based on SNP marker sets (Möhring et al 2005) and STS from resistant gene analogs (RGAs) (Hunger et al 2003). A total of 1287 combinations (99 primer sets and 13 restriction endonucleases) were tested in an effort to detect polymorphism between the parental lines.…”

Section: Methodsmentioning

confidence: 99%

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Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgarisL.)

Taguchi

Kubo

Takahashi

et al. 2011

G3 Genes|Genomes|Genetics

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The complex inheritance of resistance to Cercospora leaf spot (CLS), the most severe fungal foliar disease in sugar beet, was investigated by means of quantitative trait loci (QTL) analysis. Over a three year period, recombinant inbred lines (RILs) of sugar beet (Beta vulgaris L.), generated through a cross between lines resistant (‘NK-310mm-O’) and susceptible (‘NK-184mm-O’) to CLS, were field-tested for their resistance to the pathogen. Composite interval mapping (CIM) showed four QTL involved in CLS resistance to be consistently detected. Two resistant QTL (qcr1 on chromosome III, qcr4 on chromosome IX) bearing ‘NK-310mm-O’ derived alleles promoted resistance. Across 11 investigations, the qcr1 and qcr4 QTL explained approximately 10% and over 20%, respectively, of the variance in the resistance index. Two further QTL (qcr2 on chromosome IV, qcr3 on chromosome VI) bearing ‘NK-184mm-O’ derived alleles each explained about 10% of the variance. To identify the monogenic effect of the resistance, two QTL derived from ‘NK-310mm-O’ against the genetic background of ‘NK-184mm-O’, using molecular markers. The qcr1 and qcr4 were precisely mapped as single QTL, using progenies BC5F1 and BC2F1, respectively. The qcr1 that was located near e11m36-8 had CLS disease severity indices (DSI) about 15% lower than plants homozygous for the ‘NK-184mm-O’ genotype. As with qcr1, heterozygosis of the qcr4 that was located near e17m47-81 reduced DSI by about 45% compared to homozygosis. These two resistant QTL might be of particular value in marker-assisted selection (MAS) programs in CLS resistance progression.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgarisL.)

Taguchi

Kubo

Takahashi

et al. 2011

G3 Genes|Genomes|Genetics

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“…Polymorphic AFLP peaks were named according to the selective primers used to amplify them and the size (in base pairs) of the fragments scored. Anchoring single‐nucleotide polymorphism (SNP) genotyping was performed essentially as described by Möhring et al . (2004) but with the minor adaptations described by Grimmer et al .…”

Section: Methodsmentioning

confidence: 99%

Selection and characterization of resistance to Polymyxa betae, vector of Beet necrotic yellow vein virus, derived from wild sea beet

Asher¹,

Grimmer

Mutasa-Goettgens³

2009

Plant Pathology

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The plasmodiophoromycete Polymyxa betae is an obligate root parasite that transmits Beet necrotic yellow vein virus (BNYVV), the cause of sugar beet rhizomania disease. Currently, control of this disease is achieved through the use of cultivars with monogenic ( Rz1 ) partial resistance to the virus. To improve the level and durability of this resistance, sources of resistance to the virus vector, P. betae , were sought. Over 100 accessions of the wild sea beet ( Beta vulgaris ssp. maritima ) from European coastal regions were evaluated for resistance in controlled environment tests. Quantification of P. betae biomass in seedling roots was achieved using recombinant antibodies raised to a glutathione-s-transferase expressed by the parasite in vivo . Several putative sources of resistance were identified and selected plants from these were hybridized with a male-sterile sugar beet breeding line possessing partial virus resistance ( Rz1 ). Evaluation of F 1 hybrid populations identified five in which P. betae resistance had been successfully transferred from accessions originating from Mediterranean, Adriatic and Baltic coasts. A resistant individual from one of these populations was backcrossed to the sugar beet parent to produce a BC 1 population segregating for P. betae resistance. This population was also tested for resistance to BNYVV. Amplified fragment length polymorphism and single-nucleotide polymorphism markers were used to map resistance quantitative trait loci (QTL) to linkage groups representing specific chromosomes. QTL for resistance to both P. betae and BNYVV were co-localized on chromosome IV in the BC 1 population, indicating resistance to rhizomania conditioned by vector resistance. This resistance QTL ( Pb1 ) was shown in the F 1 population to reduce P. betae levels through interaction with a second QTL ( Pb2 ) found on chromosome IX, a relationship confirmed by general linear model analysis. In the BC 1 population, vector-derived resistance from wild sea beet combined additively with the Rz1 virus resistance gene from sugar beet to reduce BNYVV levels. With partial virus resistance already deployed in a number of high-yielding sugar beet cultivars, the simple Pb1 / Pb2 two-gene system represents a valuable additional target for plant breeders.

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“…6.8 Synthesis of three-way hybrid variety including tests of combining ability for O-types and pollinators polymorphisms (AFLP); (iv) simple sequence repeats (SSR); (v) single nucleotide polymorphisms (SNP), as well as morphological and isozyme markers (Barzen et al, 1992;Pillen et al, 1992Pillen et al, , 1993Boudry et al, 1994;Barzen et al, 1995;Uphoff and Wricke, 1995;Halldén et al, 1996;Schondelmaier et al, 1996;Nilsson et al, 1997;Schumacher et al, 1997;Hansen et al, 1999;Weber et al, 1999;Rae et al, 2000;Schneider et al, 2001;Möhring et al, 2004;Grimmer et al, 2007a;Schneider et al, 2007). The specific numbering of individual chromosomes, defined in genetic linkage maps, has been standardized.…”

Section: Genetic Mapsmentioning

confidence: 99%

Root and Tuber Crops

Bradshaw¹

2010

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Multiplexed, linkage group-specific SNP marker sets for rapid genetic mapping and fingerprinting of sugar beet (Beta vulgaris L.)

Cited by 30 publications

References 29 publications

Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgarisL.)

Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgarisL.)

Selection and characterization of resistance to Polymyxa betae, vector of Beet necrotic yellow vein virus, derived from wild sea beet

Root and Tuber Crops

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