2023
DOI: 10.7554/elife.85348
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Multiplexed microfluidic screening of bacterial chemotaxis

Abstract: Microorganism sensing of and responding to ambient chemical gradients regulates a myriad of microbial processes that are fundamental to ecosystem function and human health and disease. The development of efficient, high-throughput screening tools for microbial chemotaxis is essential to disentangling the roles of diverse chemical compounds and concentrations that control cell nutrient uptake, chemorepulsion from toxins, and microbial pathogenesis. Here, we present a novel microfluidic multiplexed chemotaxis de… Show more

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Cited by 4 publications
(10 citation statements)
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“…2a) was chosen due to its well-documented motility and chemotaxis [2123], and its prevalence in marine microbial communities [24]. The chemotactic response of V. alginolyticus to both infected and uninfected exudates was assayed at six time points throughout the infection cycle using an established microfluidic device [20, 25] (Fig. 2b).…”
Section: Resultsmentioning
confidence: 99%
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“…2a) was chosen due to its well-documented motility and chemotaxis [2123], and its prevalence in marine microbial communities [24]. The chemotactic response of V. alginolyticus to both infected and uninfected exudates was assayed at six time points throughout the infection cycle using an established microfluidic device [20, 25] (Fig. 2b).…”
Section: Resultsmentioning
confidence: 99%
“…2d). The cell population response is compactly characterised by the accumulation index [13, 20] (Fig. 2e), β ( t ) = ( n I ( t ) − n U ( t )) / ( N I + N U ), where n I,U ( t ) are the instantaneous number of cells at time t within 200 μ m of the upper (infected) and lower (uninfected) boundaries (Fig.…”
Section: Resultsmentioning
confidence: 99%
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