Multiplexed mRNA Sensing and Combinatorial-Targeted Drug Delivery Using DNA-Gold Nanoparticle Dimers

Kyriazi, Maria Eleni; Giust, Davide; El‐Sagheer, Afaf H.; Lackie, Peter M.; Muskens, Otto L.; Brown, Tom; Kanaras, Antonios G.

doi:10.1021/acsnano.7b08620

Cited by 118 publications

(83 citation statements)

References 49 publications

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“…DNA-Gold Nanoparticles for mRNA Detection DNA-Gold Nanoparticles for mRNA Detection were manufactured as previously detailed 13 . The sequences used are detailed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids

Martín¹,

Kyriazi

Lanham³

et al. 2019

Preprint

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There is a wealth of data indicating human bone marrow derived stromal cells (HBMSCs) contain the skeletal stem cell (SSC) with the potential to differentiate along the osteogenic, adipogenic and chondrogenic lineages leading to significant interest as potential clinical therapies. However, despite these advances, current methods to isolate skeletal stem cells (SSCs) from human tissues are difficult as no single specific marker has been identified. Hence, limited understanding of SSC fate, immuno-phenotype and simple selection criteria proved to be limiting factors in the widespread clinical application of these cells. While a range of cell surface markers can enrich for SSCs, none of the proposed markers, alone, isolate single cells with the ability to form bone, cartilage, and adipose tissue in humans. In this work, we have developed a methodology to use oligonucleotide-coated gold nanoparticles to identify cells in human bone marrow displaying specific mRNA signatures to isolate and enrich for the SSCs.

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“…DNA-Gold Nanoparticles for mRNA Detection DNA-Gold Nanoparticles for mRNA Detection were manufactured as previously detailed 13 . The sequences used are detailed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids

Martín¹,

Kyriazi

Lanham³

et al. 2019

Preprint

Self Cite

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“…Recently, many researchers have sought to develop an in situ fluorescence analysis strategy for sensing diverse biological samples such as DNA, RNA, and proteins using MNPs [57][58][59][60][61]. Representatively, Au nanoparticles have frequently been used for biomolecular detection by the fluorescence resonance energy transfer (FRET) effect [62].…”

Section: Metallic Nanoparticle-based Fluorescence Sensing Strategies mentioning

confidence: 99%

Metallic Nanoparticle-Based Optical Cell Chip for Nondestructive Monitoring of Intra/Extracellular Signals

et al. 2020

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The biosensing platform is noteworthy for high sensitivity and precise detection of target analytes, which are related to the status of cells or specific diseases. The modification of the transducers with metallic nanoparticles (MNPs) has attracted attention owing to excellent features such as improved sensitivity and selectivity. Moreover, the incorporation of MNPs into biosensing systems may increase the speed and the capability of the biosensors. In this review, we introduce the current progress of the developed cell-based biosensors, cell chip, based on the unique physiochemical features of MNPs. Mainly, we focus on optical intra/extracellular biosensing methods, including fluorescence, localized surface plasmon resonance (LSPR), and surface-enhanced Raman spectroscopy (SERS) based on the coupling of MNPs. We believe that the topics discussed here are useful and able to provide a guideline in the development of new MNP-based cell chip platforms for pharmaceutical applications such as drug screening and toxicological tests in the near future.

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“…9 Similar observations were made by us 10 and by others. [11][12][13] Endosomal entrapment is a problem for two reasons. First, only particles that have escaped from the endosome can detect cytosolic mRNAs.…”

Section: 8mentioning

confidence: 99%

Re-Evaluating the Spherical-Nucleic-Acid Technology

Czarnek¹,

Mason²,

Haimovich³

et al. 2018

Preprint

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Multiplexed mRNA Sensing and Combinatorial-Targeted Drug Delivery Using DNA-Gold Nanoparticle Dimers

Cited by 118 publications

References 49 publications

Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids

Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids

Metallic Nanoparticle-Based Optical Cell Chip for Nondestructive Monitoring of Intra/Extracellular Signals

Re-Evaluating the Spherical-Nucleic-Acid Technology

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