Multiplexed optical pathogen detection with lab‐on‐a‐chip devices

Schulze, Holger; Giraud, G.; Crain, Jason; Bachmann, Till T.

doi:10.1002/jbio.200910009

Cited by 29 publications

(20 citation statements)

References 92 publications

(117 reference statements)

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“…[3][4][5] The integration of such techniques in modern bioanalytical devices such as micro total analysis systems a͒ Author to whom correspondence should be addressed. Electronic mail: gerard.giraud@ed.ac.uk b͒ Electronic mail: till.bachmann@ed.ac.uk BIOMICROFLUIDICS 5, 024116 ͑2011͒ ͑TAS͒ and point of care testing 6 ͑POCT͒ requires advances in minimally invasive technology to enable efficient concentration, trapping and separation of RNA molecules.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic manipulation of ribosomal RNA

et al. 2011

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The manipulation of ribosomal RNA ͑rRNA͒ extracted from E. coli cells by dielectrophoresis ͑DEP͒ has been demonstrated over the range of 3 kHz-50 MHz using interdigitated microelectrodes. Quantitative measurement using total internal reflection fluorescence microscopy of the time dependent collection indicated a positive DEP response characterized by a plateau between 3 kHz and 1 MHz followed by a decrease in response at higher frequencies. Negative DEP was observed above 9 MHz. The positive DEP response below 1 MHz is described by the ClausiusMossotti model and corresponds to an induced dipole moment of 3300 D with a polarizability of 7.8ϫ 10 −32 F m 2 . The negative DEP response above 9 MHz indicates that the rRNA molecules exhibit a net moment of Ϫ250 D, to give an effective permittivity value of 78.5 0 , close to that of the aqueous suspending medium, and a relatively small surface conductance value of ϳ0.1 nS. This suggests that our rRNA samples have a fairly open structure accessible to the surrounding water molecules, with counterions strongly bound to the charged phosphate groups in the rRNA backbone. These results are the first demonstration of DEP for fast capture and release of rRNA units, opening new opportunities for rRNA-based biosensing devices.

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Section: Introductionmentioning

confidence: 99%

Dielectrophoretic manipulation of ribosomal RNA

et al. 2011

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“…Although many existing ‘lab-on-a-chip’ platforms have small dimensions, they still required various ancillary equipment to be operational, effectively functioning as a ‘chip-in-a-lab’ [66–68]. To enable automated sample preparation at POC, microfluidics technology, or fluidic manipulation at the micron scale, is the core technology that will integrate the reagent transfer, target isolation, and sample mixing steps in a multi-layered cartridge containing channels, valves, and reagent reservoirs [67, 69–72].…”

Section: Sample Preparation Is a Technology Bottleneckmentioning

confidence: 99%

Biosensor diagnosis of urinary tract infections: a path to better treatment?

Mach

Wong

Liao

2011

Trends in Pharmacological Sciences

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Urinary tract infection (UTI) is among the most common bacterial infections and poses a significant healthcare burden. The standard culture-based diagnosis of UTI has a typical delay of two to three days. In the absence of definitive microbiological diagnosis at the point of care, physicians frequently initiate empirical broad-spectrum antibiotic treatment, which has contributed to the emergence of resistant pathogens. Biosensors are emerging as a powerful diagnostic platform for infectious diseases. Similar to how blood glucose sensors revolutionized the management of diabetes and pregnancy tests are now conducted at home, biosensors are poised to significantly improve UTI diagnosis. Biosensors are amenable to integration with microfluidic technology for point-of-care applications. This review focuses on promising biosensor technology for UTI diagnosis, including pathogen identification and antimicrobial susceptibility testing and hurdles in the translation of biosensor technology from bench to bedside.

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“…In addition to the use of digital cameras for cell imaging, POC testing for infectious disease has already been demonstrated with LOCs that offer rapid diagnosis of HIV, malaria, tuberculosis, hepatitis, schistosomiasis, and Dengue (9 ). These analyses can be performed by LOC genomics, proteomics, and immunoassays, such as those used in the GreeneChip and the stand-alone, self-powered integrated microfluidic blood-analysis system (SIMBAS).…”

Section: Clinical Applications For Locmentioning

confidence: 99%