Multiplexed Optogenetic Stimulation of Neurons with Spectrum‐Selective Upconversion Nanoparticles

Lin, Xudong; Wang, Ying; Chen, Xian; Yang, Runhuai; Wang, Zixun; Feng, Jingyu; Wang, Haitao; Lai, King Wai Chiu; He, Jufang; Wang, Feng; Shi, Peng

doi:10.1002/adhm.201700446

Cited by 69 publications

(62 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After recovering to its original shape, the velocity of the robot restores to the original level very quickly. Also, as reported by former works, NIR irradiation of a similar power level (less than 25 mw cm −2 ) has no significant effects on the biological samples. According to our experimental observations, the robot could be sustained for a good number of circles (up to 60 in our experiments or even more) as long as the cells are within the 3 weeks and capable of self‐sustaining without the need for external power supplies, emphasizing the unique advantages of the biohybrid system.…”

Section: Resultssupporting

confidence: 82%

A Remotely Controlled Transformable Soft Robot Based on Engineered Cardiac Tissue Construct

Han

et al. 2019

Small

Self Cite

View full text Add to dashboard Cite

Many living organisms undergo conspicuous or abrupt changes in body structure, which is often accompanied by a behavioral change. Inspired by the natural metamorphosis, robotic systems can be designed as reconfigurable to be multifunctional. Here, a tissue‐engineered transformable robot is developed, which can be remotely controlled to assume different mechanical structures for switching locomotive function. The soft robot is actuated by a muscular tail fin that emulates the swimming of whales and works as a cellular engine powered by the synchronized contraction of striated cardiac microtissue constructs. For a transition of locomotive behavior, the robot can be optically triggered to transform from a spread to a retracted form, which effectively changes the bending stiffness of the tail fins, thus minimizing the propulsion output from the “tail fin” and effectively switching off the engine. With the unprecedented controllability and responsiveness, the transformable robot is implemented to work as a cargo carrier for programmed delivery of chemotherapeutic agents to selectively eradicate cancer cells. It is believed that the realization of the transformable concept paves a pathway for potential development of intelligent biohybrid robotic systems.

show abstract

Section: Resultssupporting

confidence: 82%

A Remotely Controlled Transformable Soft Robot Based on Engineered Cardiac Tissue Construct

Han

et al. 2019

Small

Self Cite

View full text Add to dashboard Cite

show abstract

“…Optogenetics relies on the expression of exogenous light activated ion channels, including the blue light-activated channelrhodopsin-2 (ChR2), that causes depolarization, or the orange-light activated halorhodopsin, which causes hyperpolarization, of the membrane potential of brain cells of interest. Despite these great advances, improvements to the method are constantly being developed (Rein and Deussing, 2012; Lim et al, 2013; Lin et al, 2017; Chen et al, 2018). The use of optogenetics in vivo requires delivery of light into the brain, which is most commonly done through implantation of fiber optic waveguides (fibers) or light emitting diodes (LEDs).…”

Section: Introductionmentioning

confidence: 99%

LSO:Ce Inorganic Scintillators are Biocompatible with Neuronal and Circuit Function

Bartley

Abiraman

Stewart

et al. 2019

Preprint

View full text Add to dashboard Cite

2Optogenetics is widely used in neuroscience to control neural circuits. However, non-invasive methods for light 3 delivery in brain are needed to avoid physical damage caused by current methods. One potential strategy could 4 employ x-ray activation of radioluminescent particles (RPLs), enabling localized light generation within the brain. 5RPLs composed of inorganic scintillators can emit light at various wavelengths depending upon composition. 6Cerium doped lutetium oxyorthosilicate (LSO:Ce), an inorganic scintillator that emits blue light in response to x-7 ray or UV stimulation, could potentially be used to control neural circuits through activation of channelrhodopsin-8 2 (ChR2), a light-gated cation channel. Whether inorganic scintillators themselves negatively impact neuronal 9 processes and synaptic function is unknown, and was investigated here using cellular, molecular, and 10 electrophysiological approaches. As proof of principle, we applied UV stimulation to 4 µm LSO:Ce particles 11 during whole-cell recording of CA1 pyramidal cells in acutely prepared hippocampal slices from mice that 12 expressed ChR2 in glutamatergic neurons. We observed an increase in frequency and amplitude of spontaneous 13 excitatory postsynaptic currents (EPSCs), indicating UV activation of ChR2 and excitation of neurons.14 Importantly, we found that LSO:Ce particles have no effect on survival of primary mouse cortical neurons, even 15 after 24 hours of exposure. In extracellular dendritic field potential recordings, we observed no change in strength 16 of basal glutamatergic transmission up to 3 hours of exposure to LSO:Ce microparticles. However, there was a 17 slight decrease in the frequency of spontaneous EPSCs in whole-cell voltage-clamp recordings from CA1 18 pyramidal cells, with no change in current amplitudes. No changes in the amplitude or frequency of spontaneous 19 inhibitory postsynaptic currents (IPSCs) were observed. Finally, long term potentiation (LTP), a synaptic 20 modification believed to underlie learning and memory and a robust measure of synaptic integrity, was 21 successfully induced, although the magnitude was slightly reduced. Together, these results show LSO:Ce particles 22 are biocompatible even though there are modest effects on baseline synaptic function and long-term synaptic 23 plasticity. Importantly, we show that light emitted from LSO:Ce particles is able to activate ChR2 and modify 24 synaptic function. Therefore, LSO:Ce inorganic scintillators are potentially viable for use as a new light delivery 25 system for optogenetics.

show abstract

“…Moreover, this study provided an innovative tetherless upconversion optogenetic strategy for brain stimulation in behaving rats. Notably, another work by Shi et al applied the similar upconversion based strategy for multiplexed optogenetic neural stimulation both in vitro and in vivo by fabricating spectrum‐selective UCNPs . Such unique technique would significantly benefit both fundamental physiological regulation and translational neuroscience research.…”

Section: Advances Of Upconversion Optogenetics In Nir Membrane Ion Chmentioning

confidence: 99%

Section: Advances Of Upconversion Optogenetics In Nir Membrane Ion Chmentioning

confidence: 99%

Near‐Infrared Manipulation of Membrane Ion Channels via Upconversion Optogenetics

Wang

et al. 2018

Adv. Biosys.

View full text Add to dashboard Cite

Membrane ion channels are ultimately responsible for the propagation and integration of electrical signals in the nervous, muscular, and other systems. Their activation or malfunctioning plays a significant role in physiological and pathophysiological processes. Using optogenetics to dynamically and spatiotemporally control ion channels has recently attracted considerable attention. However, most of the established optogenetic tools (e.g., channelrhodopsins, ChRs) for optical manipulations, are mainly stimulated by UV or visible light, which raises the concerns of potential photodamage, limited tissue penetration, and high‐invasive implantation of optical fiber devices. Near‐infrared (NIR) upconversion nanoparticle (UCNP)‐mediated optogenetic systems provide great opportunities for overcoming the problems encountered in the manipulation of ion channels in deep tissues. Hence, this review focuses on the recent advances in NIR regulation of membrane ion channels via upconversion optogenetics in biomedical research. The engineering and applications of upconversion optogenetic systems by the incorporation multiple emissive UCNPs into various light‐gated ChRs/ligands are first elaborated, followed by a detailed discussion of the technical improvements for more precise and efficient control of membrane channels. Finally, the future perspectives for refining and advancing NIR‐mediated upconversion optogenetics into in vivo even in clinical applications are proposed.

show abstract

Multiplexed Optogenetic Stimulation of Neurons with Spectrum‐Selective Upconversion Nanoparticles

Cited by 69 publications

References 41 publications

A Remotely Controlled Transformable Soft Robot Based on Engineered Cardiac Tissue Construct

A Remotely Controlled Transformable Soft Robot Based on Engineered Cardiac Tissue Construct

LSO:Ce Inorganic Scintillators are Biocompatible with Neuronal and Circuit Function

Near‐Infrared Manipulation of Membrane Ion Channels via Upconversion Optogenetics

Contact Info

Product

Resources

About