Multiplicação in vitro de bromélias Aechmea aquilega e Bromelia balansae

Dias, Gabriel Junio da Silva; Silva, Letícia Ferreira da; Carneiro, Leonardo Alves; Sousa, Cleiton Mateus

doi:10.34117/bjdv6n4-062

Cited by 4 publications

(4 citation statements)

References 9 publications

(9 reference statements)

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“…The mean survival of initial explants was higher than 95% in all treatments, denoting that the plant aerial part is a promising initial explant for multiplication of V. seubertiana, as it provides high sprouting rate and can be used for new subcultures, which probable explains its use in other studies evaluating Vellozia species (FREITAS NETO, 2009;BORGES, 2015). Similarly, whole-plant explants from seed germination have been used for rosette species, such as bromeliads (DIAS et al, 2020).…”

Section: Resultsmentioning

confidence: 88%

Micropropagation of Vellozia seubertiana (Velloziaceae)

Pinto

Lima-Brito

2023

Rev. Caatinga

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Velloziaceae is one of the main families in the floristic composition of the Campos Rupestres Montane Savanna ecoregion of the Chapada Diamantina Highland, Bahia, Brazil, and has species with significant ornamental potential and resistance to climate changes. The species Vellozia seubertiana stands out for its beautiful flowers and stems covered by leaf sheaths that ensure protection when in contact with fire. However, there is no information on its propagation, conservation, and physiology, which justifies this study. The objective of this work was to establish a micropropagation protocol for V. seubertiana. Seeds were disinfected and inoculated in Murashige and Skoog culture medium (MS) with half salt concentration (MS½) for in vitro establishment. Plantlets established in vitro were used to induce sprouting in MS½ supplemented with 6-benzylaminopurine (BAP; 0.00, 4.44, 8.88, and 17.76 µM) and 1-naphthaleneacetic acid (NAA; 0.00 and 2.22 µM). The shoots obtained were inoculated in MS½ containing activated charcoal (0.0 and 1.0 g L−¹) and indole-3-butyric acid (IBA; 0.00 and 2.22 µM) for rooting. The rooted plantlets were acclimated under greenhouse conditions. A mean of 5.7 shoots were generated through organogenesis in medium containing 8.75 µM of BAP and 2.22 µM of NAA. The use of activated charcoal resulted in the highest means for aerial part and root lengths in the in vivo rooting phase. Acclimated plants reached 75% survival at 60 days after transplanting to ex vitro conditions. The results indicate that micropropagation is a promising technique for the production of V. seubertiana seedlings.

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Section: Resultsmentioning

confidence: 88%

Micropropagation of Vellozia seubertiana (Velloziaceae)

Pinto

Lima-Brito

2023

Rev. Caatinga

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“…Some bromeliads grown in vitro in a medium without PGRs had higher percentages of shoot-producing plants than N. minutum. For example, Bromelia balansae had 25% after 249 days (Dias et al, 2020), and Sincoraea mucugensis had 77% after 60 days (Lima, Brito, & Santana, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…PGRs are involved in cell differentiation during new shoot formation (Faria et al, 2018) and are considered, in many cases, essential for micropropagation. Cytokinins and auxins, such as 6-benzylaminopurine (BAP) and 1-naphthylacetic acid (NAA), respectively, are the PGRs most widely used for producing Bromeliaceae sprouts in vitro (Silva et al, 2012;Faria et al, 2018;Dias, Silva, Carneiro, & Sousa, 2020).…”

Section: Introductionmentioning

confidence: 99%

Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)

Teixeira,

Nievola

2023

Acta Sci. Biol. Sci.

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Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This bromeliad takes a long time to sprout in vitro, and 10% of the plants produce shoots in a culture medium without plant growth regulators (PGRs). When subcultured in a PGR-free medium, these individualized shoots can sprout like the mother plant. The Murashige and Skoog basal medium (MS) containing 1.0 mg L-1 of 6-benzylaminopurine (BAP) promoted the induction of adventitious shoots in greater than 90% of the plants after 240 days of culture with an average of more than eight shoots per plant. Approximately 100% of the in vitro-produced shoots survived after acclimatization, reaching the flowering stage. Therefore, our results showed that in vitro regeneration of N. minutum depends on the cultivation period and that plants with a higher sprouting capacity can be selected and used as micropropagation matrices, contributing to the production of this endangered bromeliad.

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“…Another determining factor is that, while temperate climate flowers have an average pot life from 5 to 10 days, tropical flowers last from 20 to 30 days (Ferreira and Anacleto, 2019). Among the tropical flowers that have been gaining prominence in the trade, the bromeliads stand out, as they have characteristics favorable to commercialization (Nasser et al, 2019;Dias et al, 2020) such as beauty, exotic flowers and inflorescences (Tamaki et al, 2020), in addition to their intense colors (Anacleto and Negrelle, 2019).…”

Section: Introductionmentioning

confidence: 99%

GERMINATION AND SURVIVAL OF Nidularium innocentii Lem. (BROMELIACEAE), ORNAMENTAL BROMELIAD ON LOW-COST SUBSTRATES FOR CULTIVATION: AN ALTERNATIVE TO EXTRATIVISM

Anacleto,

Jesus

2023

Rev. Inst. Florest.

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The extractivism of bromeliads in Paraná for ornamental purposes has been occurring since the 70's, due to the easy access in the forests, the high demand of consumers, and the small number of producers in the region. Among the species of this group stands out Nidularium innocentii Lem. as the second most extracted species with commercial purpose. The reversal of this situation may be associated with the implantation of agronomic crops, but there are few and sparse studies on the germination of this species. Thus, in this context a study was carried out in order to evaluate seed germination and survival on different substrates. Six alternative types of substrates that were easily accessible to the rural producer were tested: peat, coconut husk fiber, plantmax®, earthworm humus, humid pine bark and sieved agricultural soil. The experiment was carried out in a completely randomized design, with four replicates and 25 seeds per experimental unit, at room temperature, in a greenhouse, with sprinkler irrigation for a period of 90 days. Nidularium inocentii showed germination on average at 7 days and maximum germination point was reached at 15.2 days, with an average of 65.8% of germination. Among the substrates tested, coconut husk fiber, peat and humid pine bark obtained the best results on seedling survival after seed germination. The study confirms the feasibility of sexual reproduction of the species for agronomic crops using low cost and locally abundant substrates.

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Multiplicação in vitro de bromélias Aechmea aquilega e Bromelia balansae

Abstract: Multiplicação in vitro de bromélias Aechmea aquilega e Bromelia balansae In vitro multiplication of bromeliads Aechmea aquilega and Bromelia balansae

Cited by 4 publications

References 9 publications

Micropropagation of Vellozia seubertiana (Velloziaceae)

Micropropagation of Vellozia seubertiana (Velloziaceae)

Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)

GERMINATION AND SURVIVAL OF Nidularium innocentii Lem. (BROMELIACEAE), ORNAMENTAL BROMELIAD ON LOW-COST SUBSTRATES FOR CULTIVATION: AN ALTERNATIVE TO EXTRATIVISM

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