MULTIPLICATION OF POTATO LEAF ROLL VIRUS IN THE APHID MYZUS PERSICAE (SULZ.)

Abstract

Insect‐to‐insect transmission of potato leaf roll virus proved possible by injecting either juice or haemolymph from viruliferous Myzus persicae into virus‐free ones. In serial transmission experiments, in which injection of haemolymph was practised, the virus could be carried through as many as 15 successive aphid‐to‐aphid passages without access of inoculated insects to any plants that are susceptible to leaf roll. The theoretical dilution of original virus used for the inoculation in the 15th passage would have been approximately 10−21. It is concluded that such persistence of virus in the aphids could only be explained on the assumption that the insect itself sustains reproduction of the virus. Zusammenfassung 1. Das Blattrollvirus der Kartoffel konnte durch Injektion von Preßsaft oder Blut virusinfizierter Myzus persicae (Sulz.) auf virusfreie Insekten übertragen werden. 2. Bei Serien‐Übertragungen unter Verwendung von Blutinjektionen blieb das Virus in 15 aufeinander folgenden Generationen ursprünglich virusfreier Blattläuse erhalten. Hierbei wurde nach der Injektion als Zwischenwirt der blattrollunempfindliche chinesische Kohl benutzt. 3. Die theoretische Verdünnung der ursprünglichen Virusmenge pro Blattlaus betrug nach der 15. Passage ungefähr 10−21. Injektion des auf 1: 104 verdünnten Blutes virusinfizierter Läuse ergab dagegen in keinem Fall Infektionsfähigkeit (Tabelle III und IV). 4. Die Existenz einer Latenz‐Periode bei der Übertragung konnte nach Injektion kleiner Virusmengen bewiesen werden. Verdünnung des injizierten Blutes verlängerte diese Periode (Tabelle I und II). 5. Diese Befunde deuten auf Vermehrung des Virus innerhalb der Blattläsen. 6. Wenn virusinfizierte Blattläuse auf chinesischem Kohl gehalten werden, zeigt ihre Infektionsfähigkeit deutlich periodische Schwankungen (Fig. 1).

Hundred years of Koch's Postulates and the history of etiology in plant virus research

“…1928) and later applied to aphids by Rochow (1960), or by injecting them with the suspension, as first for leafhoppers by Storey (1932) and Black and Brakke (1952) and later for aphids by Stegwee and Ponsen (1958). In fact, the technique of membrane feeding was first developed by Storey (1932) for demonstrating the filterability of maize streak virus which is not mechanically transmissible from plant to plant.…”

The effect of potato leafroll virus on the biology of Myzus persicae

“…The experiments were carried out with the isolate of PLRV used by Stegwee and Ponsen (1958). The virus was maintained in P.floridana by repeated aphid transfers.…”

“…) (Stegwee and Ponsen, 1958), affects the development of this aphid. Janssen (1929) and Arenz (1951) reported a more rapid increase in number of M. persicae on leafroll-diseased potato plants than on healthy ones.…”

The biological transmission of potato leafroll virus by Myzus persicae

“…Since PLRV multiplies in its vector (Stegwee and Ponsen, 1958) this increase during the larval stage of M. persicae must be due to virus multiplication in the aphid. In the imaginal stage of the aphid no virus multiplication probably takes place, although the virus could be recovered from the haemolymph (Stegwee and Ponsen, 1958). According to Harrewijn and Noordink (in prep.…”

“…Moreover, Day (1955) found that the percentage of PLRV-diseased test plants increased with the time the aphids had stayed on the immune host Brassica chinensis. On the other hand, Stegwee and Ponsen (1958) demonstrated big variations in the infection ability of viruliferous aphids during their life.…”