A technique to rapidly scan an optical focus inside a turbid medium is attractive for various biomedical applications. Time-reversed ultrasonically encoded (TRUE) optical focusing has previously demonstrated light focusing into a turbid medium, using both analog and digital devices. Although the digital implementation can generate a focus with high energy, it has been time consuming to scan the TRUE focus inside a sample. Here, by sweeping the frequencies of both ultrasound and light, we demonstrate a multiplex recording of ultrasonically encoded wavefronts, accelerating the generation of multiple TRUE foci. Using this technique, we obtained a 2-D image of a fluorescent target centered inside a turbid sample having a thickness of 2.4 transport mean free paths. Fluorescence imaging is widely used to obtain biological images by scanning an optical focus. 1 However, due to scattering, optical focusing using an ordinary lens is limited to shallow depths of one transport mean free path, or 1 l t 0 ($1 mm in human skin), 2 beyond which scattering both reduces the amount of light arriving at the target and blurs the resulting image.One way to overcome this limitation is to use timereversed ultrasonically encoded (TRUE) focusing. 3,4 In TRUE focusing, a focused ultrasonic (US) pulse, applied inside a turbid sample, frequency modulates (or encodes) light within the acoustic volume. A phase-conjugated version of the encoded wavefront is then generated, using either analog or digital phase conjugate mirrors (PCMs). 3-6 Digital PCMs, consisting of a camera and a spatial light modulator (SLM), are attractive for higher energy focusing. [4][5][6] To record the encoded wavefront, a reference beam interferes with the encoded light on the camera, causing the intensity of the interferogram to beat at their difference frequency. 6 The encoded wavefront is extracted from the beat, and then its phase-conjugated version is reproduced using the SLM. Upon back-propagation to the sample, the phase-conjugated beam forms an optical focus at the original location of the ultrasound volume.It has been previously shown that TRUE focusing improves the resolution, thereby allowing deep fluorescence imaging beyond 1 l t 0 inside a scattering medium. 4-6 However, one of the challenges of applying digital TRUE focusing for imaging was the long time (several seconds) taken to generate a single optical focus. 5,6 The low signal-to-noise ratio (SNR) of the encoded-light detection typically mandates that multiple frames of interferograms be recorded and averaged to obtain a single encoded wavefront.Here, we propose a method called frequency-swept TRUE focusing, which takes advantage of the multiple recorded frames used for encoded-light detection to accelerate TRUE focal scanning. By sweeping the frequency of both the ultrasound and the light at the same time, we achieve simultaneous recording of multiple wavefronts, corresponding to different positions along the acoustic axis, without sacrificing SNR and using the same number of camera frames. A simi...