Multipotency of equine mesenchymal stem cells derived from synovial fluid

Murata, Daiki; Miyakoshi, Daiske; Hatazoe, Takashi; Miura, Naoki; Tokunaga, Shigehiko; Fujiki, Makoto; Nakayama, Koichi; Misumi, Kazuhiro

doi:10.1016/j.tvjl.2014.07.029

Cited by 41 publications

(66 citation statements)

References 15 publications

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“…Surprisingly, donor age had no signficiant effect on initial CFU concentrations or cell proliferation. The cell populations expanded in monolayer culture expressed osteogenic, adipogenic and chondrogenic phenotypes under standard in vitro culture conditions, consistent with findings in similar studies [4,5,10,12], and reflecting the multi-lineage potential requirement for MSCs.…”

Section: Discussionsupporting

confidence: 87%

“…The presence of MSCs in synovial fluids was first reported in 2004 [8], and subsequent studies have corroborated this finding in several model species, including the horse [9][10][11][12]. The proliferative and differentiation capacities of synovial fluidderived progenitor cells have varied between studies [9][10][11][12][13], but in all cases, these cells were capable of chondrogenesis.…”

Section: Introductionmentioning

confidence: 82%

“…The proliferative and differentiation capacities of synovial fluidderived progenitor cells have varied between studies [9][10][11][12][13], but in all cases, these cells were capable of chondrogenesis. However, the specific chondrogenic lineage of synovial fluidderived chondroprogenitors (SF-CP) has not been defined.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Chen¹,

Bianchessi²,

Pondenis³

et al. 2016

Stem Cell Biol Res

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Background: Progenitor cells exist in most tissues and body fluids. Synovial fluid chondroprogenitor cells have been described in several species; however, the specific phenotypic characteristics of these cells have not been defined. This study addressed the impacts of joint location and donor age variation on synovial fluid chondroprogenitor numbers, and determined whether synovial fluid chondroprogenitors express hypertrophic characteristics or a non-endochondral phenotype in chondrogenic culture. Methods: Synovial fluid was aspirated from the joints of healthy adult horses, and cells in the aspirates were expanded through two monolayer passages. The number of colony-forming units (CFU) in each aspirate was assessed after 14 days. Population doublings (PD) and PD times were calculated during the subsequent two passages. Passage 3 cells were used to determine osteogenic and adipogenic capacities, or were transferred to pellet cultures in chondrogenic medium containing TGF-β1 or BMP-2. Bone marrowderived MSCs and primary articular chondrocytes were cultured under similar conditions to provide reference values for chondrogenesis. Chondrogenesis was assessed by measuring collagen type II protein and sulfated glycosaminoglycan secretion, expression of chondrogenic mRNAs, and induction of ALP activity. Results: CFU counts varied considerably, but the majority of aspirates contained <50 CFU/ml. PDs were consistently between 2.0 and 3.0 during both passages. PD times varied from 0.2-0.4 days. Proliferation was not significantly influenced by anatomical location or donor age. Equine synovial fluid progenitors expressed osteogenic, adipogenic and chondrogenic phenotypes under appropriate conditions. Under chondrogenic conditions, synovial fluid cells increased collagen and aggrecan mRNA expression to levels comparable to bone marrow MSCs, although collagen type II protein secretion was less than half that of articular chondrocytes. In contrast to bone marrow MSCs, synovial fluid chondroprogenitors did not express collagen type X or increase ALP activity in response to TGF-β1 or BMP-2. Consistent with these observations, Runx2 and Mef2C expression in synovial fluid chondroprogenitors was 20-40 fold lower than in bone marrow MSCs. Conclusions: Synovial fluid chondroprogenitors are capable of robust non-hypertrophic chondrogenesis, whether stimulated by TGF-β1 or by BMP-2. These results indicate that synovial fluid cells are phenotypically suitable for articular cartilage repair/regeneration, although strategies to accelerate cell proliferation and synthesize a functional cartilage matrix in vivo will be required for clinically feasible cellbased therapies.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 82%

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Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Chen¹,

Bianchessi²,

Pondenis³

et al. 2016

Stem Cell Biol Res

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“…LS-CTMs podem ser coletadas com métodos minimamente invasivos através de artrocentese e requer menos processamento do que a MS, desta forma, reduz o risco de contaminação celular quando em cultura MURATA et al, 2014).…”

Section: Scutt; Stolzing 2006)unclassified

“…Atualmente, surgiram alguns trabalhos utilizando células humanas ARUFE et al, 2009;KRAWETZ et al, 2012;SEKIYA et al, 2012) e somente um utilizando células de equinos (MURATA et al, 2014). Este último comparou as populações celulares derivadas de articulações hígidas com as obtidas de articulações com fratura intraarticular ou com osteocondrite dissecante, com o intuito de investigar a capacidade de proliferação, características fenotípicas e de diferenciação celular.…”

Section: Introductionunclassified

<b>Estudo <i>in vitro</i> do potencial de diferenciação condrogênico e osteogênico de células mesenquimais obtidas de líquido e membrana sinovial de equinos</b>

Fülber¹

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Electrospun poly(d,l‐lactide)/gelatin/glass‐ceramics tricomponent nanofibrous scaffold for bone tissue engineering

Bochicchio

Barbaro

Bonis

et al. 2020

J Biomedical Materials Res

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Electrospun scaffolds are emerging as extracellular matrix (ECM)mimicking structures for tissue engineering thanks to their nanofibrous architecture. For the development of suitable electrospun scaffolds for bone tissue engineering, the addition of inorganic components has been implemented with the aim to confer important bioactivity like osteoinduction, osteointegration, and cell adhesion to the scaffolds. In this context, we propose a tricomponent electrospun scaffold composed of poly(d,l‐lactide), gelatin and RKKP glass‐ceramics. The bioactive RKKP glass‐ceramic system has attracted interest, due to the presence of ions such as La3+ and Ta5+, which turned out to be valuable as growth supporting agents for bones. In this work, RKKP glass‐ceramics were embedded inside the microfibers of electrospun scaffolds and the structural and biological properties were investigated. Our results showed that the glass‐ceramic microparticles were uniformly distributed in the fibrous structure of the scaffold. Furthermore, the glass‐ceramics promoted biomineralization of the scaffolds and improved cell viability and osteogenic differentiation. The mineralized layer formed on RKKP‐containing scaffolds after incubation in simulated body fluid medium has been shown to be hydroxyapatite by Raman spectroscopy and X‐ray diffraction. The results on differentiation studies of canine adipose‐derived mesenchymal stem cells grown on the electrospun scaffolds suggest that on varying the content of RKKP in the scaffold, it is possible to drive the differentiation toward chondrogenic or osteogenic commitment. The presence of ions, like La3+ and Ta5+, in the RKKP embedded polymeric composite scaffolds could play a role in supporting cell growth and promoting differentiation.

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Multipotency of equine mesenchymal stem cells derived from synovial fluid

Cited by 41 publications

References 15 publications

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

<b>Estudo <i>in vitro</i> do potencial de diferenciação condrogênico e osteogênico de células mesenquimais obtidas de líquido e membrana sinovial de equinos</b>

Electrospun poly(d,l‐lactide)/gelatin/glass‐ceramics tricomponent nanofibrous scaffold for bone tissue engineering

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