Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation

Semenov, Oleg V.; Koestenbauer, Sonja; Riegel, Mariluce; Zech, Nikolas; Zimmermann, Roland; Zisch, Andreas H.; Malek, Antoine

doi:10.1016/j.ajog.2009.10.869

Cited by 103 publications

(71 citation statements)

References 41 publications

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“…Recently, multipotent MSCs have also been isolated in placental tissues, including the amnion and chorion layers of the amniotic membrane,21, 22, 23, 24 and these amniotic membrane derived stem cells have demonstrated the ability to regulate immune cells though secretion of various immunomodulatory cytokines 25, 26, 27, 28…”

Section: Introductionmentioning

confidence: 99%

Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

Massee

Chinn

Lei³

et al. 2015

J Biomed Mater Res

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Human‐derived placental tissues have been shown in randomized clinical trials to be effective for healing chronic wounds, and have also demonstrated the ability to recruit stem cells to the wound site in vitro and in vivo. In this study, PURION® Processed dehydrated human amnion/chorion membrane allografts (dHACM, EpiFix®, MiMedx Group, Marietta, GA) were evaluated for their ability to alter stem cell activity in vitro. Human bone marrow mesenchymal stem cells (BM‐MSCs), adipose derived stem cells (ADSCs), and hematopoietic stem cells (HSCs) were treated with soluble extracts of dHACM tissue, and were evaluated for cellular proliferation, migration, and cytokine secretion. Stem cells were analyzed for cell number by DNA assay after 24 h, closure of an acellular zone using microscopy over 3 days, and soluble cytokine production in the medium of treated stem cells was analyzed after 3 days using a multiplex ELISA array. Treatment with soluble extracts of dHACM tissue stimulated BM‐MSCs, ADSCs, and HSCs to proliferate with a significant increase in cell number after 24 h. dHACM treatment accelerated closure of an acellular zone by ADSCs and BM‐MSCs after 3 days, compared to basal medium. BM‐MSCs, ADSCs, and HSCs also modulated endogenous production of a number of various soluble signals, including regulators of inflammation, mitogenesis, and wound healing. dHACM treatment promoted increased proliferation and migration of ADSCs, BM‐MSCs, and HSCs, along with modulation of secreted proteins from those cells. Therefore, dHACM may impact wound healing by amplifying host stem cell populations and modulating their responses in treated wound tissues. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1495–1503, 2016.

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Section: Introductionmentioning

confidence: 99%

Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

Massee

Chinn

Lei³

et al. 2015

J Biomed Mater Res

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“…MSCs previously isolated from human placenta 35 were cultured in DMEM with Glutamax supplemented with 1 mM sodium pyruvate, HEPES at pH 7.5, 5% penicillin/streptomycin and 10% fetal calf serum. Cells were used between passages 7 and 16.…”

Section: Fxiiia-mediated Biomolecule Tethering To Hydrogels In Two DImentioning

confidence: 99%

In situ cell manipulation through enzymatic hydrogel photopatterning

et al. 2013

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The physicochemical properties of hydrogels can be manipulated in both space and time through the controlled application of a light beam. However, methods for hydrogel photopatterning either fail to maintain the bioactivity of fragile proteins and are thus limited to short peptides, or have been used in hydrogels that often do not support three-dimensional (3D) cell growth. Here, we show that the 3D invasion of primary human mesenchymal stem cells can be spatiotemporally controlled by micropatterning the hydrogel with desired extracellular matrix (ECM) proteins and growth factors. A peptide substrate of activated transglutaminase factor XIII (FXIIIa)-a key ECM crosslinking enzyme-is rendered photosensitive by masking its active site with a photolabile cage group. Covalent incorporation of the caged FXIIIa substrate into poly(ethylene glycol) hydrogels and subsequent laser-scanning lithography affords highly localized biomolecule tethering. This approach for the 3D manipulation of cells within gels should open up avenues for the study and manipulation of cell signalling.

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“…MSCs have been isolated from many different tissues, including bone marrow (Oyajobi et al. 1999;Majumdar et al, 2000;Prockop et al, 2001;Smith et al, 2004;Titorencu et al, 2007;Wolfe et al, 2008;Gronthos and Zannettino et al, 2008;Phadnis et al, 2011;Bao et al, 2011), adipose tissue (Katz et al, 2005;Baptista et al, 2009;Caviggioli et al, 2009;Baer et al, 2010;Bruyn et al, 2010;Estes et al, 2010;Tucker, Bunnell, 2011), peripheral blood (Kassis et al, 2006), umbilical cord blood (Erices et al, 2000;Rosada et al, 2003;Hutson et al, 2005;Reinisch et al, 2007;Bieback and Klüter et al, 2007;Perdikogianni et al, 2008;Zhang et al, 2011), banked umbilical cord blood (Phuc et al, 2011), umbilical cords (Cutler et al, 2010Farias et al, 2011), umbilical cord membranes (Deuse et al, 2010;Kita et al, 2010), umbilical cord veins (Santos et al, 2010), Wharton's jelly from the umbilical cord (Zeddou et al, 2010;Peng et al, 2011), placenta (Miao et al, 2006;Battula et al, 2007;Huang et al, 2009;Semenov et al, 2010;Pilz et al, 2011), decidua basalis (Macias et al, 2010;Lu et al, 2011), the ligamentum flavum , amniotic fluid (Feng et al, 2009;…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Stem Cell Therapy for Islet Regeneration

Pham¹

2011

Stem Cells in Clinic and Research

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Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation

Cited by 103 publications

References 41 publications

Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

In situ cell manipulation through enzymatic hydrogel photopatterning

Stem Cell Therapy for Islet Regeneration

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