Multipotent Mesenchymal Stem Cells with Immunosuppressive Activity Can Be Easily Isolated from Dental Pulp

Pierdomenico, Laura; Bonsi, Laura; Calvitti, Mario; Rondelli, Damiano; Arpinati, Mario; Chirumbolo, Gabriella; Becchetti, Ennio; Marchionni, Cosetta; Alviano, Francesco; Fossati, Valentina; Staffolani, N; Franchina, Michele; Grossi, Alberto; Bagnara, Gian Paolo

doi:10.1097/01.tp.0000173794.72151.88

Cited by 482 publications

(424 citation statements)

References 27 publications

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“…Existing protocols for isolating pulp tissue from human tooth had been used various instruments such as pliers (bone forceps) 9 , extirpation needle 29 , Gracey curette 30 , dental fissure burs 4 , etc. Those methods are time-consuming, while considering low level of overheating of pulp by adding water continuously.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Karamzadeh

Eslaminejad

Aflatoonian

2012

JoVE

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Section: Discussionmentioning

confidence: 99%

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Karamzadeh

Eslaminejad

Aflatoonian

2012

JoVE

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“…This could be very relevant for cell therapy because stem cells from dental tissues are known to be easily accessible for extraction and well-tolerated upon grafting, due to their immunesuppressive properties (Pierdomenico et al, 2005). Furthermore, DPSCs are non-tumourigenic even after their immortalisation by telomerase overexpression (Wilson et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Notch/Wnt cross-signalling regulates stemness of dental pulp stem cells through expression of neural crest and core pluripotency factors

Uribe-Etxebarria¹,

Luzuriaga²,

García-Gallastegui³

et al. 2017

eCM

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Dental pulp stem cells (DPSCs) from adult teeth express neural crest (NC) markers together with core transcriptional factors associated with stem cell pluripotency, such as Oct4a, Sox2, Rex1, Stella/ Dppa3, Ssea1/Fut4, Lin28 and Nanog. The possibility to boost the natural stemness features of DPSCs by mild methods, that do not involve gene and/or chromatin modification or gene transfection, is highly desirable for cell therapy. Canonical Wnt and Notch are two highly conserved developmental signalling pathways that are involved in NC emergence and stem cell self-renewal. We determined that both pathways coordinate to regulate the expression of core pluripotency and NC factors in DPSCs. Pharmacological inhibition of the Notch pathway for 48 h, by the γ-secretase inhibitor N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT), abolished the expression of NC and core factors. In addition, it induced a silencing of the canonical Wnt signalling and a clear reduction in the stemness potential of DPSCs, as shown by a reduced ability to generate mature, fully differentiated osteoblasts and adipocytes. Conversely, pharmacological activation of the Wnt pathway for 48 h, by either the glycogen synthase kinase 3 beta (GSK3-β) inhibitor 6-bromoindirubin-3´-oxime (BIO) or the human recombinant protein Wnt-3a, not only largely increased the expression of NC and core factors, but also increased the efficiency of DPSCs to differentiate into mature osteoblasts and adipocytes. These results showed that a short preconditioning activation of Wnt/Notch signalling by small molecules and/or recombinant proteins enhanced the stemness and potency of DPSCs in culture, which could be useful for optimising the therapeutic use of these and other tissue-specific stem cells.

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“…Cells were equally positive for SH2, SH3, SH4, CD29 and CD166. These cells show osteogenic, chondrogenic and adipogenic lineages, but did not differentiate into chondrocytes [29]. Digested in collagenase/ dipase, single cell suspensions were obtained.…”

Section: Regeneration and Repair Of Pulp And/or Dental Structuresmentioning

confidence: 99%

Stem Cells: Tools for Dental Tissues Regeneration

Goldberg

2017

JDHODT

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Multipotent Mesenchymal Stem Cells with Immunosuppressive Activity Can Be Easily Isolated from Dental Pulp

Cited by 482 publications

References 27 publications

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Notch/Wnt cross-signalling regulates stemness of dental pulp stem cells through expression of neural crest and core pluripotency factors

Stem Cells: Tools for Dental Tissues Regeneration

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