Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons

Amoh, Yasuyuki; Li, Lingna; Katsuoka, Kensei; Penman, Sheldon; Hoffman, Robert M.

doi:10.1073/pnas.0501263102

Cited by 394 publications

(376 citation statements)

References 16 publications

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“…However, this is a difficult problem, and although the task is urgent, it will be some time before it is possible to guarantee sufficient safety to enable stem cell use in clinical settings. Prior to any clinical trial of human CNS disorders using iPS cells, it will be essential to pre-evaluate each iPS cell clone carefully to be able to guarantee a safety level equal to other types of cells, such as hair follicle stem cells [45][46][47][48][49][50][51], multipotent skin-derived precursor cells [52,53], fetusderived neural stem/progenitor cells [54], and neural crest stem cells [55]. Moreover, experiments on primates will be essential, as preclinical studies before transplantation can be tried in SCI patients [56].…”

Section: Future Prospects and Tasksmentioning

confidence: 99%

Cell Therapy for Spinal Cord Injury by Neural Stem/Progenitor Cells Derived from iPS/ES Cells

et al. 2011

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Reports of functional recovery from spinal cord injury after the transplantation of rat fetus-derived neural stem cells and embryonic stem cells has raised great expectations for the successful clinical use of stem cell transplantation therapy. However, the ethical issues involved in destroying human embryos or fertilized oocytes to obtain stem cells have been a major obstacle to developing clinically useful stem cell sources, and the transplantation of stem cells isolated from other human embryonic tissues has not yet been developed for use in clinical applications. Recently, induced pluripotent stem cells, which can serve as a source of cells for autologous transplantation, have been attracting a great deal of attention as a clinically viable alternative to stem cells obtained directly from tissues. In this review, we outline the neural induction of mouse embryonic stem cells and induced pluripotent stem cells, their therapeutic efficacy in spinal cord injury, and their safety in vivo.

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Section: Future Prospects and Tasksmentioning

confidence: 99%

Cell Therapy for Spinal Cord Injury by Neural Stem/Progenitor Cells Derived from iPS/ES Cells

et al. 2011

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“…Apparently, further studies are necessary to clarify the in vivo mechanisms restricting the multipotential capability of Sox10þ NC cells. Also, in this context, multipotential stem cells established from dissociated skin (Fernandes et al, 2004;Amoh et al, 2005) could possibly be generated from a differentiated subpopulation of NC cells such as Sox10þ/Kitþ melanoblast precursors.…”

Section: Developmental Dynamicsmentioning

confidence: 99%

Neural crest cells retain their capability for multipotential differentiation even after lineage‐restricted stages

Motohashi

Yamanaka

Chiba

et al. 2011

Developmental Dynamics

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Multipotency of neural crest cells (NC cells) is thought to be a transient phase at the early stage of their generation; after NC cells emerge from the neural tube, they are specified into the lineage‐restricted precursors. We analyzed the differentiation of early‐stage NC‐like cells derived from Sox10‐IRES‐Venus ES cells, where the expression of Sox10 can be visualized with a fluorescent protein. Unexpectedly, both the Sox10+/Kit− cells and the Sox10+/Kit+ cells, which were restricted in vivo to the neuron (N)‐glial cell (G) lineage and melanocyte (M) lineage, respectively, generated N, G, and M, showing that they retain multipotency. We generated mice from the Sox10‐IRES‐Venus ES cells and analyzed the differentiation of their NC cells. Both the Sox10+/Kit− cells and Sox10+/Kit+ cells isolated from these mice formed colonies containing N, G, and M, showing that they are also multipotent. These findings suggest that NC cells retain multipotency even after the initial lineage‐restricted stages. Developmental Dynamics 240:1681–1693, 2011. © 2011 Wiley‐Liss, Inc.

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“…4 Our laboratory discovered hair-follicle-associated pluripotent (HAP) stem cells, located in the bulge area, 5 HAP stem cells from mouse expressed nestin and could differentiate to neurons, glia, keratinocytes, smooth-muscle cells, and melanocytes in vitro. 6,7 HAP stem cells from mouse could effect nerve repair 8,9 and spinal cord regeneration 10 in mouse models. We then demonstrated that mouse HAP stem cells differentiate to beating cardiac-muscle cells.…”

Section: Introductionmentioning

confidence: 99%

Human hair-follicle associated pluripotent (hHAP) stem cells differentiate to cardiac-muscle cells

et al. 2016

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We have previously demonstrated that nestin-expressing hair follicle-associated-pluripotent (HAP) stem cells are located in the bulge area. HAP stem cells have been previously shown to differentiate to neurons, glial cells, keratinocytes, smooth-muscle cells, melanocytes and cardiac-muscle cells in vitro. Subsequently, we demonstrated that HAP stem cells could effect nerve and spinal cord regeneration in mouse models, differentiating to Schwann cells and neurons. In previous studies, we established an efficient protocol for the differentiation of cardiac-muscle cells from mouse HAP stem cells. In the present study, we isolated the upper part of human hair follicles containing human HAP (hHAP) stem cells. The upper parts of human hair follicles were suspended in DMEM containing 10% FBS where they differentiated to cardiac-muscle cells as well as neurons, glial cells, keratinocytes and smooth-muscle cells. This method is appropriate for future use with human hair follicles to produce hHAP stem cells in sufficient quantities for future heart, nerve and spinal cord regeneration in the clinic.

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Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons

Cited by 394 publications

References 16 publications

Cell Therapy for Spinal Cord Injury by Neural Stem/Progenitor Cells Derived from iPS/ES Cells

Cell Therapy for Spinal Cord Injury by Neural Stem/Progenitor Cells Derived from iPS/ES Cells

Neural crest cells retain their capability for multipotential differentiation even after lineage‐restricted stages

Human hair-follicle associated pluripotent (hHAP) stem cells differentiate to cardiac-muscle cells

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