Multiprimer PCR System Diagnosis of Pulmonary Tuberculosis in Cochabamba, Bolivia

Parrado, Rudy; Lozano, Daniel; García, Lineth; Torrico, Mary Cruz; Delgado, R; Torrico, Faustino; Laserna, Monica; Reithinger, Richard

doi:10.1128/jcm.01575-07

Cited by 1 publication

(3 citation statements)

References 7 publications

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“…Several molecular-based methods used for the diagnosis of tuberculosis in respiratory specimens have been evaluated for their applicability to the diagnosis of TBM (23). Many of these methods have failed with CSF samples, mainly due to the fact that the number of bacilli typically present in TBM is low and that amplification inhibitors are present in the CSF.…”

Section: Discussionmentioning

confidence: 99%

“…The PCR system also has been used as an epidemiological tool in strain classification (23,24). The most widely used target sequence for the diagnosis of tuberculosis has been the IS6110 insertion element, present in a different number of copies in the genome of species of the M. tuberculosis complex (12,35) and PCR techniques based on this sequence have shown to be useful for diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“…The analysis of the genomic sequence of M. tuberculosis (19) and studies on comparative genomics (20,21) have identified major deletions that specifically characterize different species of the M. tuberculosis complex, and are useful in differentiating these from non-tuberculous mycobacteria (22)(23)(24)(25). Some studies have reported the use of PCR and quantitative PCR to identify M. tuberculosis in the CSF of patients with TBM, with varying sensitivity and specificity (25,26).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Mycobacterium tuberculosis in the cerebrospinal fluid of patients with meningitis using nested PCR

Rios-Sarabia

Hernández-González

González-y-Merchand

et al. 2016

International Journal of Molecular Medicine

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Abstract. Tuberculous meningitis (TBM) is the most severe form of tuberculosis. It is caused by Mycobacterium tubercu losis (M. tuberculosis; MT) and it is very difficult to diagnose. The symptoms are similar to other infectious neurological diseases, such as neurocysticercosis, neuroborreliosis, or herpes viral infection. The aim of this study was to identify tuberculosis (TB) in cases of meningitis with clinical and laboratory evidence suggestive of TBM, and to confirm our findings with molecular tests for TB infection. We recruited patients with neurological symptoms who were examined at the neurology services of Hospitals of Instituto Mexicano del Seguro Social (IMSS) in Mexico City. A total of 144 consecutive patients with suggestive infectious meningitis were initially included; 94 cases of meningitis with clinical and laboratory evidence suggestive of TBM were included, but only 50 of these cases fulfilled the criteria for probable TBM. As the controls, we included 50 cases of meningitis with clinical and laboratory evidence suggestive of non-TBM. Cerebrospinal fluid (CSF) was collected from all 100 patients (cases and controls) and tested for TB by multiplex and nested PCR analyses. Nested PCR detected 0.1 fg of M. tuberculosis DNA. TB infection was confirmed with molecular tests in 49 patients from the 50 cases suggestive of TBM and in 1 of the 50 non-TBM cases. The analysis exhibited a sensitivity of 98.0%, a specificity of 92.0%, a positive predictive value of 88.0% and a negative predictive value of 98.0%. The use CSF for the analyses proved to be effective for the rapid diagnosis of TBM using a developed system of multiplex and nested PCR analyses in patients presenting neurological symptoms.

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Section: Discussionmentioning

confidence: 99%