Multiprotein Immunoassay Arrays Fabricated by Microcontact Printing

Abstract

Microarrays containing up to three different proteins were fabricated by microcontact printing (µCP) techniques and tested as a detection system for specific antibodies. After fabrication, immunoassays were successfully performed using the patterned protein microarrays. The developed immunoassays were characterized by fluorescence microscopy and scanning probe microscopy. The characterization revealed the quality of the protein deposition and indicated a high degree of selectivity for the targeted antigenantibody interactions. The results of this study suggest that µCP is an inexpensive and effective way to fabricate biologically active substrates that can be of use for multiple reagentless immunosensor applications.

Site‐Specific Patterning of Biomolecules and Quantum Dots on Functionalized Surfaces Generated by Plasma‐Enhanced Chemical Vapor Deposition

“…[7,8] The latter two applications exemplify the increasing coordination between materials science and biology for future-generation advanced materials. Plasma-enhanced chemical vapor deposition (PECVD) shows great promise for strengthening this aforementioned materials science and biology intersection.…”

“…Spatial binding of biomolecules and quantum dots to PECVD-patterned substrates is demonstrated. Currently, surface patterning of biomolecules involves techniques such as dip-pen nanolithography, [10][11][12] electron- [13] and ion-beam lithography, [14,15] polydimethylsiloxane stamping, [8,16] ink-jet printing, [17] nanoparticle self-assembly, [18] chemical selectivity on patterned gold, [19] and atomic force microscopy (AFM) mediated nanografting. [20,21] These techniques encounter inherent limitations and drawbacks for biomolecular patterning.…”

Microfabrication of Patterned Co-cultures for Controllable Cell–Cell Interfaces

“…Sequential use of μCP with different protein types results in patterned multiprotein structures [ 56 ]. Although sequential μCP is very practical and still in use for co-culturing, using stamps one after another may not result in high-fi delity structures in terms of alignment [ 57 ].…”

Multicolor microcontact printing of proteins on nanoporous surface for patterned immunoassay

“…Since then, lCP has been extended to incorporate many applications, including the patterning of functional proteins for immunoassays and biosensors (Bernard et al 2000;Pattani et al 2008). Furthermore, to enhance the diagnostic power of immunoassays, multiple proteins can be patterned on the same substrate and has been demonstrated in a variety of ways (Inerowicz et al 2002;Crozatier et al 2006;Ghosh et al 2008). The ability to print multiple proteins on a single substrate and on such a small scale is important in creating an efficient point of care detection and analysis systems.…”