Multiresistant Gram-Negative Pathogens

Köck, Robin; Herr, C; Kreienbrock, Lothar; Schwarz, Štefan; Tenhagen, Bernd‐Alois; Walther, Birgit

doi:10.3238/arztebl.m2021.0184

Cited by 15 publications

(12 citation statements)

References 132 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This plasmid has been identified in both human and veterinary Pasteurellaceae pathogens, including P. multocida, one of the few members of this family capable of infecting a range of animals, including humans. It should be noted that our results do not preclude the possibility of transfer of MGE-associated AMR determinants from members of the Pasteurellaceae to other co-resident bacterial species found within the same animal host, some of which may be zoonotic and therefore present a greater threat to human health (Köck et al, 2021).…”

Section: Discussionmentioning

confidence: 67%

Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species

et al. 2022

View full text Add to dashboard Cite

Mobile genetic elements (MGEs) and antimicrobial resistance (AMR) drive important ecological relationships in microbial communities and pathogen-host interaction. In this study, we investigated the resistome-associated mobilome in 345 publicly available Pasteurellaceae genomes, a large family of Gram-negative bacteria including major human and animal pathogens. We generated a comprehensive dataset of the mobilome integrated into genomes, including 10,820 insertion sequences, 2,939 prophages, and 43 integrative and conjugative elements. Also, we assessed plasmid sequences of Pasteurellaceae. Our findings greatly expand the diversity of MGEs for the family, including a description of novel elements. We discovered that MGEs are comparable and dispersed across species and that they also co-occur in genomes, contributing to the family’s ecology via gene transfer. In addition, we investigated the impact of these elements in the dissemination and shaping of AMR genes. A total of 55 different AMR genes were mapped to 721 locations in the dataset. MGEs are linked with 77.6% of AMR genes discovered, indicating their important involvement in the acquisition and transmission of such genes. This study provides an uncharted view of the Pasteurellaceae by demonstrating the global distribution of resistance genes linked with MGEs.

show abstract

Section: Discussionmentioning

confidence: 67%

Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In Deutschland werden mcr-Gene in 8 % der Schweine-und 25 % der Putenfleischproben nachgewiesen [16] bzw. bei am Schlachthof untersuchten Proben enthielten 9,1 %, 4,7 %, 2,5 %, 0,4 % der E. coli von Puten, Masthähnchen, -kälbern, -schweinen mcr [17]. Doch werden die Erreger von dort auf den Menschen übertragen?…”

Section: Colistinunclassified

“…Doch werden die Erreger von dort auf den Menschen übertragen? Hierzu fehlen Daten, da Colistin meist nicht Teil des Routinetestpanels in der humanmedizinischen Mikrobiologie ist; mcr-vermittelte Colistin-Resistenz scheint bei klinischen E.-coli-Isolaten jedoch selten zu sein [17].…”

Section: Colistinunclassified

“…Wie es aussieht, spielt die zoonotische Übertragung über Fleisch und direkten Tierkontakt im Moment eine geringe Rolle, zumindest in Deutschland. Allerdings sind immer mehr Fernreisende mit mcr-E. coli bei Reiserückkehr besiedelt: aus Indien 3 %, Mekka 9 %, diversen Zielen 5-11 % [17]. Das ist ein schlechtes Zeichen, denn über die Quellen in den Reiseländern kann nur spekuliert werden; Wasser oder Lebensmittel und Kontakt zur einheimischen Bevölkerung erscheinen jedoch nicht unwahrscheinlich.…”

Section: Colistinunclassified

See 1 more Smart Citation

Antibiotika – Einsatz und Resistenz in Human- und Veterinärmedizin

Köck¹

2021

Krankenhaushygiene up2date

Self Cite

View full text Add to dashboard Cite

“…Possible explanations for the current rise of MDR‐GNB carriage might be the progressive production and use of antibiotics in human and veterinary medicine, the growing number of asylum seekers from countries with high MDR‐GNB prevalence (Krüger et al, 2016), the ascending quantity of international travels and mobility increases (Meyer et al, 2012), the rising number of long‐term care facilities (Gruber et al, 2013), and a frequent contact with livestock (Ewers et al, 2012). Further aspects are foodborne transmission and human‐to‐human transmission in households as well as pet‐to‐human transmission in households, and transmission in healthcare facilities such as hospitals (Köck et al, 2021). Extended‐spectrum‐β‐lactamases (ESBL) are among the most common resistance mechanisms (Bradford, 2001).…”

Section: Introductionmentioning

confidence: 99%

Novel insights into pivotal risk factors for rectal carriage of extended-spectrum-β-lactamase-producing enterobacterales within the general population in Lower Saxony, Germany

Symanzik

Hillenbrand

Stasielowicz

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

To estimate the prevalence of extended-spectrum-βlactamase (ESBL)producing enterobacterales (ESBL-E) carriage in the general population of Lower Saxony, Germany, and to identify risk factors for being colonized. Methods and Results: Participants were recruited through local press and information events. Detection of ESBL-E by culture was conducted using ESBL-selective chromagar plates containing third-generation cephalosporins. Identification of pathogens was performed using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF)_technology on Vitek mass spectrometry. Antibiotic susceptibility testing was conducted by microdilution (Vitek II) and an ESBL confirmation assay was carried out using a combination disk test. Of 527 randomly collected stool samples from healthy volunteers, 5.5% were tested positive for ESBL-E. Post-stratification for age and gender yielded a similar population estimate (5.9%). People traveling abroad and taking antibiotics had the greatest rectal ESBL-E carriage. Conclusions: Potential risk factors (eg, working in healthcare facilities, recent inpatient stay) did not attribute to rectal ESBL-E carriage as other factors (eg, travelling, taking antibiotics). Rectal ESBL-E carriage within the general population seems to be high. Significance and Impact of the Study: The known risk factors for carriage with MDRO might not be fully applicable to ESBL-E and require further examination in order to develop effective strategies for the prevention of ESBL-E dissemination within the general population.

show abstract

Multiresistant Gram-Negative Pathogens

Cited by 15 publications

References 132 publications

Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species

Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species

Antibiotika – Einsatz und Resistenz in Human- und Veterinärmedizin

Novel insights into pivotal risk factors for rectal carriage of extended-spectrum-β-lactamase-producing enterobacterales within the general population in Lower Saxony, Germany

Contact Info

Product

Resources

About