Multiresistant serotype O 12Pseudomonas aeruginosa: evidence for a common strain in Europe

Abstract

SUMMARYA survey was made of serotype association and multiple antibiotic resistance in strains of Pseudomonas aeruginosa in Europe. Of 208 epidemiologically distinct strains from 16 laboratories in 10 countries, 48 were resistant to carbenicillin (MIC > 128 ,ug/ml) and gentamicin (MIC > 4 ,ag/ml), and 12 of these strains were of serotype 0 12. Representative 0 12 strains from different countries were compared with two multiresistant 0 12 strains isolated 5 years apart. from a British burns unit and the antibiotic sensitive serotype reference strain. All 0 12 strains were similar by phage and pyocin typing but lysogenic phage profiles indicated that two strains (the later burns isolate and the serotype strain) were distinct from the others. Electrophoretic characterization of outer membrane proteins, lipopolysaccharides and esterase enzymes corroborated the relationship of the strains and restriction fragment length polymorphism of DNA fragments hybridized with a cDNA probe copy of rRNA from P. aeruginosa provided further proof of their relatedness. We propose that the uniformity of characters of multiresistant 0 12 P. aeruginosa in Europe is suggestive of a common origin for the strains.

Molecular Epidemiology of Pseudomonas aeruginosa Colonization in a Burn Unit: Persistence of a Multidrug-Resistant Clone and a Silver Sulfadiazine-Resistant Clone

“…The MDR AFLP35 strain belonged to serogroup O12. Epidemic strains are frequently of serotypes O11 and O12 (13,14,15,19,43,47,55). Detection of the simultaneous presence of three distinct P. aeruginosa O12 strains, one of which was an environmental strain from the BWC, by AFLP analysis illustrates the advantage of genotyping over serotyping.…”

Use of ribotyping in epidemiological surveillance of nosocomial outbreaks

“…These include Serratia marcescens (26) (55,121), for example, then neither a phenotypic nor a genotypic technique is likely to be useful and the investigation will have to rely on classical epidemiological studies. This is even more true when, among the various clones of a species, only one is virulent, e.g., H. influenzae biogroup aegyptus for Brazilian purpuric fever (70) or the E. coli strain responsible for hemolytic uremic syndrome (92).…”

Complexity ofPseudomonas aeruginosainfection in cystic fibrosis: combined results from esterase electrophoresis and rDNA restriction fragment length polymorphism analysis

“…Each distinct combination of patterns was used to define a ribotype. Altogether, 16 ribotypes were identified (A-P) ( epidemiologic analysis of P. aeruginosa [10,[21][22][23]. In this study, we have combined esterase electrophoretic typing and ribotyping.…”

“…E. coli rRNA. Indeed, Pitt and colleagues [10] have used these two methods concomitantly for characterization of multiresistant serotype 0 12 P. aeruginosa strains. All the studied P. aeruginosa isolates could be precisely and unambiguously characterized by esterase electrophoresis and RFLP of rDNA regions.…”

“…genome fingerprinting by field inversion gel electrophoresis (FIGE) [2] and restriction fragment length polymorphism (RFLP) in the exotoxin A [3][4][5][6][7] or pilin gene regions [4,5,8] have been used as molecular approaches to P. aeruginosa epidemiology in CF. Esterase electrophoretic typing [9][10][11] and RFLP of rDNA regions [10] have been shown to provide discriminative data for epidemiological screening in non-CF patients' P. aeruginosa strains. The present study makes use of these two methods to differentiate isolates within and between 23 P. aeruginosa chronic lung infections in CF patients.…”