Multiscale design and synthesis of biomimetic gradient protein/biosilica composites for interfacial tissue engineering

Guo, Jian Ting; Li, Chunmei; Ling, Shengjie; Huang, Wenwen; Chen, Ying; Kaplan, David L.

doi:10.1016/j.biomaterials.2017.08.025

Cited by 55 publications

(44 citation statements)

References 85 publications

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“…Some tissues in the body present pronounced gradients in ECM composition and/or cell population, particularly those at interfaces such as the osteochondral interface, the ligament‐to‐bone interface, and the tendon‐to‐bone insertion site . Conventional methods rely on the use of anisotropic scaffolds containing distinctive or continual sections with properties matching those in the native tissues to be engineered .…”

Section: Emerging Evolutions In Bioprintingmentioning

confidence: 99%

3D Bioprinting: from Benches to Translational Applications

Heinrich

Liu

Jimenez

et al. 2019

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Over the last decades, the fabrication of 3D tissues has become commonplace in tissue engineering and regenerative medicine. However, conventional 3D biofabrication techniques such as scaffolding, microengineering, and fiber and cell sheet engineering are limited in their capacity to fabricate complex tissue constructs with the required precision and controllability that is needed to replicate biologically relevant tissues. To this end, 3D bioprinting offers great versatility to fabricate biomimetic, volumetric tissues that are structurally and functionally relevant. It enables precise control of the composition, spatial distribution, and architecture of resulting constructs facilitating the recapitulation of the delicate shapes and structures of targeted organs and tissues. This Review systematically covers the history of bioprinting and the most recent advances in instrumentation and methods. It then focuses on the requirements for bioinks and cells to achieve optimal fabrication of biomimetic constructs. Next, emerging evolutions and future directions of bioprinting are discussed, such as freeform, high-resolution, multimaterial, and 4D bioprinting. Finally, the translational potential of bioprinting and bioprinted tissues of various categories are presented and the Review is concluded by exemplifying commercially available bioprinting platforms. BioprintingThe ORCID identification number(s) for the author(s) of this article can be found under https://doi.

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Section: Emerging Evolutions In Bioprintingmentioning

confidence: 99%

3D Bioprinting: from Benches to Translational Applications

Heinrich

Liu

Jimenez

et al. 2019

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“…The whole free‐standing protein system can be removed from the container and used for further post‐processing (Figure d). Our previous studies revealed that the positively charged amino acids dominated the silicification process, while the silk is negative charged . As a result, the silica nanoparticles grew selectively on the R5 peptide patterned surface, represented by the schematic drawing and confirmed by optical imaging and scanning electron microscopy (SEM) (Figure e).…”

Section: Resultsmentioning

confidence: 61%

“…The biosilica selective peptide‐R5, a bioinspired analog derived from the silaffin peptides that are used for silicification in Cylindrotheca fusiformis , was selected for the peptide ink since it can directly serve as a trapping agent to trigger the site‐specific in vitro silicification (Figure c). Our previous studies indicated that enzymatic cross‐linking between the silk and R5 molecular chains allowed for tight bonding between the substrate and printed peptide patterns . A freshly printed 1.2 cm wide peptide logo with micrometer resolution was visually discernable from the transparent underlying silk hydrogel ( Figure a).…”

Section: Resultsmentioning

confidence: 90%

“…These results revealed that the micropatterns had a lower content of β‐sheet. Our recent studies on the secondary structure of the R5 peptide before and after silicification characterized the R5 peptide as a random coil structure . Thus, amide I absorption in the micropatterns was mainly contributed by the R5 peptide instead of the silk amide I band at the blue regions.…”

Section: Resultsmentioning

confidence: 93%

“…By contrast, site‐specific biomineralization (in situ biosilicification) is able to form well‐organized silica structures on the biosilica selective peptide‐R5 (a bioinspired analog derived from the silaffin peptides for silica synthesis in Cylindrotheca fusiformis ) micropatterns while avoiding the use of harsh chemicals or complex processing. Furthermore, the silk fibroin (hereinafter referred to as silk) protein substrates offer biocompatibility, degradability and tunable mechanical properties to meet different requirements related to biomaterials, tissue engineering, and regenerative medicine depending on the specific goal of a project . Through these synergistic approaches, we obtained macroscopic silica patterns with micrometer resolution.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Coding Cell Micropatterns Through Peptide Inkjet Printing for Arbitrary Biomineralized Architectures

Ling

Chen

et al. 2018

Adv Funct Materials

Self Cite

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Well-designed micropatterns present in native tissues and organs involve changes in extracellular matrix compositions, cell types and mechanical properties to reflect complex biological functions. However, the design and fabrication of these micropatterns in vitro to meet task-specific biomedical applications remains a challenge. A de novo design strategy to code and synthesize functional micropatterns is presented to engineer cell alignment through the integration of aqueous-peptide inkjet printing and site-specific biomineralization. The inkjet printing provides direct writing of macroscopic biosilica selective peptide-R5 patterns with micrometer-scale resolution on the surface of a biopolymer (silk) hydrogel. This is combined with in situ biomineralization of the R5 peptide for site-specific growth of silica nanoparticles on the micropatterns, avoiding the use of harsh chemicals or complex processing. The functional micropatterned systems are used to align human mesenchymal stem cells and bovine serum albumin. This combination of peptide printing and site-specific biomineralization provides a new route for developing cost-effective micropatterns, with implications for broader materials designs.

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Composites of Proteins and 2D Nanomaterials

Demirel

Vural

Terrones

2017

Adv Funct Materials

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Recent advances in the nanotechnology of 2D materials, combined with parallel improvements in biotechnology and synthetic biology, have demonstrated that novel and more complex composite materials with desired engineered properties and optimized performance can be achieved. The expanding family of 2D crystals fosters research efforts on nonequilibrium materials such as nanostructured composites and complex heterostructures. In particular, controlled assembly of 2D crystals with biomolecules to form composites attracts special interest since it enables precise control over the physical properties of the resulting material. To facilitate the fabrication of these novel bio-inorganic composite materials by design, it is crucial to understand and control molecular interactions between 2D crystals and the complementary bio-system. In particular, protein-based materials with the ability to initiate multiple physical or chemical interactions with 2D crystals prove to be a suitable match for constructing functional bio-inorganic composites with programmable properties using molecular biology tools. In this review, a detailed survey on emerging nanostructured composites of 2D materials and proteins is presented, and a comprehensive analysis regarding their interactions is provided. Recent and potential applications along with future directions of research regarding the merge of synthetic biology with 2D systems are also discussed.

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Multiscale design and synthesis of biomimetic gradient protein/biosilica composites for interfacial tissue engineering

Cited by 55 publications

References 85 publications

3D Bioprinting: from Benches to Translational Applications

3D Bioprinting: from Benches to Translational Applications

Coding Cell Micropatterns Through Peptide Inkjet Printing for Arbitrary Biomineralized Architectures

Composites of Proteins and 2D Nanomaterials

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