Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry

Hussain, Muhammad Hammad; Mohsin, Muhammad; Zaman, Waqas Qamar; Yu, Junxiong; Zhao, Xuechao; Wei, Yanlong; Zhuang, Yingping; Mohsin, Ali; Guo, Meijin

doi:10.1016/j.synbio.2021.12.012

Cited by 13 publications

(7 citation statements)

References 179 publications

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“…(d) HPP2 glycosides are likely to exert the best inhibitory activity against elastase compared with the positive control oleanolic acid, thus clearly demonstrating these HPP2 glycosides with enhanced anti-melanogenic and antioxidant activities as prospective anti-wrinkle dermatological preparations. Further studies on both the GT kinetics of StSPGT on other related substrates and its introduction into the microbial cell factory for the in vivo production of desirable SP glycosides might be carried out [ 35 ], and the resulting biosynthetic new glycosides obtained from their biosynthetic platforms might also contribute to the glyco-expansibility of SPs with updated industrial potentials.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Biosynthesis of Simple Phenolic Glycosides as Potential Anti-Melanogenic Antioxidants

Jung

Kwon

et al. 2022

Antioxidants

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Simple phenolics (SPs) and their glycosides have recently gained much attention as functional skin-care resources for their anti-melanogenic and antioxidant activities. Enzymatic glycosylation of SP aglycone make it feasible to create SP glycosides with updated bioactive potentials. Herein, a glycosyltransferase (GT)-encoding gene was cloned from the fosmid libraries of Streptomyces tenjimariensis ATCC 31603 using GT-specific degenerate PCR followed by in silico analyses. The recombinant StSPGT was able to flexibly catalyze the transfer of two glycosyl moieties towards two SP acceptors, (hydroxyphenyl-2-propanol [HPP2] and hydroxyphenyl-3-propanol [HPP3]), generating stereospecific α-anomeric glycosides as follows: HPP2-O-α-glucoside, HPP2-O-α-2″-deoxyglucoside, HPP3-O-α-glucoside and HPP3-O-α-2″-deoxyglucoside. This enzyme seems not only to prefer UDP-glucose and HPP2 as a favorable glycosyl donor and acceptor, respectively but also differentiates the positional difference of the hydroxyl function as acceptor catalytic sites. Paired in vitro and in vivo antioxidant assays represented SPs and their corresponding glycosides as convincing antioxidants in a time- and concentration-dependent manner by scavenging DPPH radicals and intracellular ROS. Even compared to the conventional agents, HPP2 and glycoside analogs displayed improved tyrosinase inhibitory activity in vitro and still suppressed in vivo melanogenesis. Both HPP2 glycosides are further likely to exert the best inhibitory activity against elastase, eventually highlighting these glycosides with enhanced anti-melanogenic and antioxidant activities as promising anti-wrinkle hits.

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Section: Discussionmentioning

confidence: 99%

Enzymatic Biosynthesis of Simple Phenolic Glycosides as Potential Anti-Melanogenic Antioxidants

Jung

Kwon

et al. 2022

Antioxidants

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“…[120,121] Nonetheless, biocatalysis allows for fewer steps, and reactions in benign solvents such as water, low temperatures, and commonly available reactants. [122][123][124]…”

Section: Indigoidinementioning

confidence: 99%

Biosourced Monomers: Toward Sustainable Conjugated Polymers for Organic Electronics

Boivin

Dupont

Gendron

et al. 2022

Macro Chemistry & Physics

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Organic electronic materials are starting to appear in various applications, but there are still hurdles to overcome before the average consumer can get them in their hands. Right now, most polymers in the field of organic electronics are made from petroleum derivatives. However, new sources of platform chemicals and monomers are being explored. This perspective article highlights ways that have been explored to avoid the use of petrochemicals and demonstrates new pathways that can be taken to increase the sustainability of conjugated polymers in organic electronics.

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“…2,22 Motivated by the great demand, the past decades have seen substantial advances in developing microbial cell factory (MCF) to produce SdNPs (Table 1), which are believed to be promising alternatives to the traditional methods. 23 Moreover, with the fast enrichment of synthetic biology toolboxes, more and more nonconventional microorganisms are being harnessed as producing chassis, 24−26 and delicate genetic circuits that can achieve accurate and dynamic regulation are being developed. 27−29 As the second most abundant cofactor in nature, SAM not only mediates the biosynthesis of SdNPs but also is involved in the methylation of native proteins, nucleic acids, polysaccharides, hormones, phospholipids, and fatty acids in microorganisms.…”

Section: Introductionmentioning

confidence: 99%

“…The SAM-dependent natural products (SdNPs) have been widely used in pharmaceutical (such as pterostilbene, (2 S )-sakuranetin, eugenol, nocamycin, , gentamicin C, and ferulic acid), nutritional (such as vitamin B12, O -methyl anthocyanin, betaine, and biotin), cosmetic (such as methyl cinnamate and ergothioneine), flavor (such as vanillin and N -methylanthranilates), and energy (such as methyl esters) fields. The demand for SdNPs has increased dramatically in recent years. , Traditional approaches to SdNPs include chemical synthesis and crude extraction, however, suffer from low chiral selectivity and yield. , Motivated by the great demand, the past decades have seen substantial advances in developing microbial cell factory (MCF) to produce SdNPs (Table ), which are believed to be promising alternatives to the traditional methods . Moreover, with the fast enrichment of synthetic biology toolboxes, more and more nonconventional microorganisms are being harnessed as producing chassis, − and delicate genetic circuits that can achieve accurate and dynamic regulation are being developed. − …”

Section: Introductionmentioning

confidence: 99%

Improving Microbial Cell Factory Performance by Engineering SAM Availability

Lv,

Chang,

Zhang

et al. 2024

J. Agric. Food Chem.

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Methylated natural products are widely spread in nature. S-Adenosyl-L-methionine (SAM) is the secondary abundant cofactor and the primary methyl donor, which confer natural products with structural and functional diversification. The increasing demand for SAM-dependent natural products (SdNPs) has motivated the development of microbial cell factories (MCFs) for sustainable and efficient SdNP production. Insufficient and unsustainable SAM availability hinders the improvement of SdNP MCF performance. From the perspective of developing MCF, this review summarized recent understanding of de novo SAM biosynthesis and its regulatory mechanism. SAM is just the methyl mediator but not the original methyl source. Effective and sustainable methyl source supply is critical for efficient SdNP production. We compared and discussed the innate and relatively less explored alternative methyl sources and identified the one involving cheap one-carbon compound as more promising. The SAM biosynthesis is synergistically regulated on multilevels and is tightly connected with ATP and NAD(P)H pools. We also covered the recent advancement of metabolic engineering in improving intracellular SAM availability and SdNP production. Dynamic regulation is a promising strategy to achieve accurate and dynamic fine-tuning of intracellular SAM pool size. Finally, we discussed the design and engineering constraints underlying construction of SAM-responsive genetic circuits and envisioned their future applications in developing SdNP MCFs.

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Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry

Cited by 13 publications

References 179 publications

Enzymatic Biosynthesis of Simple Phenolic Glycosides as Potential Anti-Melanogenic Antioxidants

Enzymatic Biosynthesis of Simple Phenolic Glycosides as Potential Anti-Melanogenic Antioxidants

Biosourced Monomers: Toward Sustainable Conjugated Polymers for Organic Electronics

Improving Microbial Cell Factory Performance by Engineering SAM Availability

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