Multiscale Poly-(ϵ-caprolactone) Scaffold Mimicking Non-linearity in Tendon Tissue Mechanics

Banik, Brittany L.; Lewis, Gregory S.; Brown, Justin L.

doi:10.1007/s40883-016-0008-5

Cited by 26 publications

(26 citation statements)

References 37 publications

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“…Qualitative immunofluorescence images were obtained of representative MSCs at 24hrs on each of the four surfaces through first fixation of the samples in 3.7% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) followed by blocking and permeabilization in 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) with 2% bovine serum albumin (VWR, Radnor, PA, USA) and probing with CF568 phalloidin (Biotium, Freemont, CA, USA) or Atto490LS phalloidin for PEEK specifically (Sigma-Aldrich, St. Louis, MO, USA), DAPI (ThermoFisher, Waltham, MA, USA), and a primary antibody to the protein vinculin (Sigma-Aldrich, St. Louis, MO, USA) followed by a secondary antibody conjugated to Dylight 488 (ThermoFisher, Waltham, MA, USA) or Q dot 800 for PEEK specifically (ThermoFisher, Waltham, MA, USA) [16,20,27,28]. Z-projected 3D deconvolved slices of the immunostained MSCs were overlaid with a reflected differential interference contrast (DIC) image in grayscale of the surface obtained through a z-projected 3D inverse filtered stack to produce the resulting images of the MSCs on each surface.…”

Section: Methodsmentioning

confidence: 99%

Human mesenchymal stem cell morphology, migration, and differentiation on micro and nano-textured titanium

Long

Buluk

Gallagher³

et al. 2019

Bioactive Materials

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Orthopedic implants rely on facilitating a robust interaction between the implant material surface and the surrounding bone tissue. Ideally, the interface will encourage osseointegration with the host bone, resulting in strong fixation and implant stability. However, implant failure can occur due to the lack of integration with bone tissue or bacterial infection. The chosen material and surface topography of orthopedic implants are key factors that influence the early events following implantation and may ultimately define the success of a device. Early attachment, rapid migration and improved differentiation of stem cells to osteoblasts are necessary to populate the surface of biomedical implants, potentially preventing biofilm formation and implant-associated infection. This article explores these early stem cell specific events by seeding human mesenchymal stem cells (MSCs) on four clinically relevant materials: polyether ether ketone (PEEK), Ti6Al4V (smooth Ti), macro-micro rough Ti6Al4V (Endoskeleton®), and macro-micro-nano rough Ti6Al4V (nanoLOCK®). The results demonstrate the incorporation of a hierarchical macro-micro-nano roughness on titanium produces a stellate morphology typical of mature osteoblasts/osteocytes, rapid and random migration, and improved osteogenic differentiation in seeded MSCs. Literature suggests rapid coverage of a surface by stem cells coupled with stimulation of bone differentiation minimizes the opportunity for biofilm formation while increasing the rate of device integration with the surrounding bone tissue.

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Section: Methodsmentioning

confidence: 99%

Human mesenchymal stem cell morphology, migration, and differentiation on micro and nano-textured titanium

Long

Buluk

Gallagher³

et al. 2019

Bioactive Materials

Self Cite

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“…They found lower properties compared to the tendon tissue (average Young’s modulus = 35.8 MPa; failure stress < 12 MPa). They also seeded hMSCs on the scaffolds, finding increased values of proliferation and no cytotoxic effects [ 107 ]. Lin et al tried to reproduce the bone–ligament–bone complex, electrospinning PCL nanofibers on a motorized air gap conic collector setup ( Figure 13 b).…”

Section: Applications For Tendon and Ligament Regeneration And Repmentioning

confidence: 99%

“… Schematic workflow to produce multiscale hierarchically structured scaffolds for tendon and ligament tissue engineering: ( a ) Modified gap collector setup to produce a scaffold for tendon tissue regeneration (adapted from Banik et al [ 107 ], reproduced with permission. Copyright 2016, Springer Nature): (I) schematic representation of the electrospinning setup; (II) image of the motorized setup of the collector; and (III) image of the final scaffold.…”

Section: Figurementioning

confidence: 99%

Biofabrication of Electrospun Scaffolds for the Regeneration of Tendons and Ligaments

2018

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Tendon and ligament tissue regeneration and replacement are complex since scaffolds need to guarantee an adequate hierarchical structured morphology, and non-linear mechanical properties. Moreover, to guide the cells’ proliferation and tissue re-growth, scaffolds must provide a fibrous texture mimicking the typical of the arrangement of the collagen in the extracellular matrix of these tissues. Among the different techniques to produce scaffolds, electrospinning is one of the most promising, thanks to its ability to produce fibers of nanometric size. This manuscript aims to provide an overview to researchers approaching the field of repair and regeneration of tendons and ligaments. To clarify the general requirements of electrospun scaffolds, the first part of this manuscript presents a general overview concerning tendons’ and ligaments’ structure and mechanical properties. The different types of polymers, blends and particles most frequently used for tendon and ligament tissue engineering are summarized. Furthermore, the focus of the review is on describing the different possible electrospinning setups and processes to obtain different nanofibrous structures, such as mats, bundles, yarns and more complex hierarchical assemblies. Finally, an overview concerning how these technologies are exploited to produce electrospun scaffolds for tendon and ligament tissue applications is reported together with the main findings and outcomes.

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“…Also, MSCs cultured on these structures showed contact guidance and growth along the topography of the pattern. Cell differentiation toward tenogenic lineage was also observed with increase in expression of genes related to tenogenesis . Another study shows a robust method for continuous collection of polydioxanone fibers on a thin conductive wire collector.…”

Section: Electrospinning To Mimic Hierarchical Structure Of Tissuesmentioning

confidence: 90%

Electrospun Fibers for Recruitment and Differentiation of Stem Cells in Regenerative Medicine

Sankar

Sharma

Rath

et al. 2017

Biotechnology Journal

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Electrospinning is a popular technique used to mimic the natural sub-micron features of the native tissue. The ultra-fine fibers provide a favorable extracellular matrix-like environment for regulation of cellular functions. This article summarizes and reviews the current advances in electrospun fiber application and focuses on the novel strategies applied for tissue regeneration and repair. It explores the different factors affecting the attachment and proliferation of mesenchymal stem cells (MSCs) on the electrospun substrates. The influence of different features of electrospun fibers in the differentiation of MSCs into specific lineages (bone, cartilage, tendon/ligament, and nerves) has been elaborated. In addition, the different techniques to mimic the hierarchical features of tissues and its effect on cellular functions are reviewed. Additionally, the new developments like three-dimensional (3D) electrospinning, 3D spheroid double strategy and the comparative analysis of dynamic and static culture on electrospun scaffolds are discussed. With the intricate understanding of the interaction between the cells and the electrospun fiber matrix we can aim to combine the newer strategies to overcome the existing challenges and improve the potential application of electrospun fibers in the field of tissue regeneration and repair.

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Multiscale Poly-(ϵ-caprolactone) Scaffold Mimicking Non-linearity in Tendon Tissue Mechanics

Cited by 26 publications

References 37 publications

Human mesenchymal stem cell morphology, migration, and differentiation on micro and nano-textured titanium

Human mesenchymal stem cell morphology, migration, and differentiation on micro and nano-textured titanium

Biofabrication of Electrospun Scaffolds for the Regeneration of Tendons and Ligaments

Electrospun Fibers for Recruitment and Differentiation of Stem Cells in Regenerative Medicine

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