Multiscale stiffness characterisation of both healthy and osteoporotic bone tissue using subject-specific data

Prada, Daniel M.; Galvis, Andres F.; Miller, Johnathan; Foster, Jamie M.; Zavaglia, Cecília Amélia de Carvalho

doi:10.1016/j.jmbbm.2022.105431

Cited by 5 publications

(3 citation statements)

References 64 publications

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“…The EFMs were subjected to tensile loads and their mechanical responses were analyzed. Our results show that at the molecular level high mineral content renders stiffer bones, which is well-known. − , We found that the mineral tends to accumulate higher stress values, supporting most of the loads. Furthermore, an analysis of the stress distribution showed that the EFV plays a crucial role in the mechanical response of the EFMs.…”

Section: Discussionsupporting

confidence: 75%

“…These are, to our knowledge, the only all-atom molecular models to date that resemble simplified fibers in bones, as experimentally determined. ,− For further details on the inspiration for our models, see Schwarcz et al Figure 4 (reproduced in Figure 1 by Pang) and McNally et al Figure 8. Mineralization of the EFV has been considered before only in models with continuous media. − Consideration of the EFV is of fundamental importance to understand the mechanical properties of bones at the nanoscale, and even to be able to create advanced biocompatible scaffolds . Indeed, experiments have shown that most of the mineral content in bone, about 70% to 80%, is found in the EFV. ,,,,, The remaining part is found in the IFV, distributed among the gap and overlap zones.…”

Section: Introductionmentioning

confidence: 99%

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The Role of the Extrafibrillar Volume on the Mechanical Properties of Molecular Models of Mineralized Bone Microfibrils

Alcântara

Felix

Galvão

et al. 2022

ACS Biomater. Sci. Eng.

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Bones are responsible for body support, structure, motion, and several other functions that enable and facilitate life for many different animal species. They exhibit a complex network of distinct physical structures and mechanical properties, which ultimately depend on the fraction of their primary constituents at the molecular scale. However, the relationship between structure and mechanical properties in bones are still not fully understood. Here, we investigate structural and mechanical properties of all-atom bone molecular models composed of type-I collagen, hydroxyapatite (HA), and water by means of fully atomistic molecular dynamics simulations. Our models encompass an extrafibrillar volume (EFV) and consider mineral content in both the EFV and intrafibrillar volume (IFV), consistent with experimental observations. We investigate solvation structures and elastic properties of bone microfibril models with different degrees of mineralization, ranging from highly mineralized to weakly mineralized and nonmineralized models. We find that the local tetrahedral order of water is lost in similar ways in the EFV and IFV regions for all HA containing models, as calcium and phosphate ions are strongly coordinated with water molecules. We also subject our models to tensile loads and analyze the spatial stress distribution over the nanostructure of the material. Our results show that both mineral and water contents accumulate significantly higher stress levels, most notably in the EFV, thus revealing that this region, which has been only recently incorporated in all-atom molecular models, is fundamental for studying the mechanical properties of bones at the nanoscale. Furthermore, our results corroborate the well-established finding that high mineral content makes bone stiffer.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

The Role of the Extrafibrillar Volume on the Mechanical Properties of Molecular Models of Mineralized Bone Microfibrils

Alcântara

Felix

Galvão

et al. 2022

ACS Biomater. Sci. Eng.

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“…The relationship between microarchitecture features and macroscopic mechanical properties of trabecular bone has been extensively studied [25][26][27]. However, to date and to the best of our knowledge, the relationship between the scaffold microarchitecture and the microscopic mechanical environment remains unknown.…”

Section: Introductionmentioning

confidence: 99%

A New Microarchitecture-Based Parameter to Predict the Micromechanical Properties of Bone Allografts

et al. 2023

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Scaffolds are an essential component of bone tissue engineering. They provide support and create a physiological environment for cells to proliferate and differentiate. Bone allografts extracted from human donors are promising scaffolds due to their mechanical and structural characteristics. Bone microarchitecture is well known to be an important determinant of macroscopic mechanical properties, but its role at the microscopic, i.e., the trabeculae level is still poorly understood. The present study investigated linear correlations between microarchitectural parameters obtained from X-ray computed tomography (micro-CT) images of bone allografts, such as bone volume fraction (BV/TV), degree of anisotropy (DA), or ellipsoid factor (EF), and micromechanical parameters derived from micro-finite element calculations, such as mean axial strain (εz) and strain energy density (We). DAEF, a new parameter based on a linear combination of the two microarchitectural parameters DA and EF, showed a strong linear correlation with the bone mechanical characteristics at the microscopic scale. Our results concluded that the spatial distribution and the plate-and-rod structure of trabecular bone are the main determinants of the mechanical properties of bone at the microscopic level. The DAEF parameter could, therefore, be used as a tool to predict the level of mechanical stimulation at the local scale, a key parameter to better understand and optimize the mechanism of osteogenesis in bone tissue engineering.

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3D printing customised stiffness-matched meta-biomaterial with near-zero auxeticity for load-bearing tissue repair

et al. 2023

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Multiscale stiffness characterisation of both healthy and osteoporotic bone tissue using subject-specific data

Cited by 5 publications

References 64 publications

The Role of the Extrafibrillar Volume on the Mechanical Properties of Molecular Models of Mineralized Bone Microfibrils

The Role of the Extrafibrillar Volume on the Mechanical Properties of Molecular Models of Mineralized Bone Microfibrils

A New Microarchitecture-Based Parameter to Predict the Micromechanical Properties of Bone Allografts

3D printing customised stiffness-matched meta-biomaterial with near-zero auxeticity for load-bearing tissue repair

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