“…Due to its rapid deterioration, freshness has become one of the most important quality parameters of fish and fish products for the consumers, traders, and processors (Huang et al 2011). The conventional methods for fish freshness determination are usually based on principles of physical, chemical, microbiological, and sensory evaluation (Olafsdottir et al 2004), these techniques however, although reliable and accurate, are destructive, tedious, involve chemicals use, and above all require highly skilled personnel (Zaragozá et al 2012). In this regard, there is therefore the need to develop and evaluate a convenient, accurate, reliable, and environmentally friendly analytical method for fish freshness determination.…”