Multisignal control of expression of the LHCX protein family in the marine diatom<i>Phaeodactylum tricornutum</i>

Taddei, Lucilla; Stella, Giulio Rocco; Rogato, Alessandra; Bailleul, Benjamin; Fortunato, Antonio Emidio; Annunziata, Rossella; Sanges, Remo; Thaler, Michael; Lepetit, Bernard; Lavaud, Johann; Jaubert, Marianne; Finazzi, Guido; Bouly, Jean-Pierre; Falciatore, Angela

doi:10.1093/jxb/erw198

Cited by 97 publications

(128 citation statements)

References 89 publications

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“…Interestingly, when the light intervals become even shorter than the dark intervals in a fluctuating light regime, P. tricornutum is able to generate a very high NPQ (Lavaud et al ., ; Ruban et al ., ), which may not be triggered by light, but may be elicited by darkness instead. As only transcription of the Lhcx4 isoform is stimulated in the dark (Lepetit et al ., ; Nymark et al ., ) and the respective protein can induce NPQ (Taddei et al ., ), Lhcx4, together with the increased Dd + Dt content (Lavaud et al ., ), may be responsible for this particular increased NPQ capacity.…”

Section: Discussionmentioning

confidence: 99%

“…Different P. tricornutum ecotypes have different and variable NPQ capacities (Lavaud & Lepetit, ) that depend largely on the amount of Lhcx1 (Bailleul et al ., ). Additionally, there is growing evidence that two other light‐regulated isoforms, Lhcx2 and Lhcx3, may also participate in NPQ under prolonged light stress (Lepetit et al ., ; Taddei et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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The diatom Phaeodactylum tricornutum adjusts nonphotochemical fluorescence quenching capacity in response to dynamic light via fine‐tuned Lhcx and xanthophyll cycle pigment synthesis

et al. 2016

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Diatoms contain a highly flexible capacity to dissipate excessively absorbed light by nonphotochemical fluorescence quenching (NPQ) based on the light-induced conversion of diadinoxanthin (Dd) into diatoxanthin (Dt) and the presence of Lhcx proteins. Their NPQ fine regulation on the molecular level upon a shift to dynamic light conditions is unknown. We investigated the regulation of Dd + Dt amount, Lhcx gene and protein synthesis and NPQ capacity in the diatom Phaeodactylum tricornutum after a change from continuous low light to 3 d of sine (SL) or fluctuating (FL) light conditions. Four P. tricornutum strains with different NPQ capacities due to different expression of Lhcx1 were included. All strains responded to dynamic light comparably, independently of initial NPQ capacity. During SL, NPQ capacity was strongly enhanced due to a gradual increase of Lhcx2 and Dd + Dt amount. During FL, cells enhanced their NPQ capacity on the first day due to increased Dd + Dt, Lhcx2 and Lhcx3; already by the second day light acclimation was accomplished. While quenching efficiency of Dt was strongly lowered during SL conditions, it remained high throughout the whole FL exposure. Our results highlight a more balanced and cost-effective photoacclimation strategy of P. tricornutum under FL than under SL conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The diatom Phaeodactylum tricornutum adjusts nonphotochemical fluorescence quenching capacity in response to dynamic light via fine‐tuned Lhcx and xanthophyll cycle pigment synthesis

et al. 2016

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“…The light‐harvesting antenna in diatoms is large and, although it shares a high homology with the light‐harvesting complexes of higher plants and green algae, it differs with respect to pigment composition and excitation absorption properties (Wilhelm et al ., ; Ishihara et al ., ; Taddei et al ., ; Giovagnetti & Ruban, ). It is comprised largely of the pigments chlorophyll a (Chl a ) Chl c , and fucoxanthin, which are bound to fucoxanthin Chl a/c ‐binding proteins (FCPs).…”

Section: Introductionmentioning

confidence: 98%

Dark metabolism: a molecular insight into how the Antarctic sea‐ice diatom Fragilariopsis cylindrus survives long‐term darkness

et al. 2019

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Summary Light underneath Antarctic sea‐ice is below detectable limits for up to 4 months of the year. The ability of Antarctic sea‐ice diatoms to survive this prolonged darkness relies on their metabolic capability. This study is the first to examine the proteome of a prominent sea‐ice diatom in response to extended darkness, focusing on the protein‐level mechanisms of dark survival. The Antarctic diatom Fragilariopsis cylindrus was grown under continuous light or darkness for 120 d. The whole cell proteome was quantitatively analysed by nano‐ LC−MS / MS to investigate metabolic changes that occur during sustained darkness and during recovery under illumination. Enzymes of metabolic pathways, particularly those involved in respiratory processes, tricarboxylic acid cycle, glycolysis, the Entner−Doudoroff pathway, the urea cycle and the mitochondrial electron transport chain became more abundant in the dark. Within the plastid, carbon fixation halted while the upper sections of the glycolysis, gluconeogenesis and pentose phosphate pathways became less active. We have discovered how F. cylindrus utilises an ancient alternative metabolic mechanism that enables its capacity for long‐term dark survival. By sustaining essential metabolic processes in the dark, F. cylindrus retains the functionality of the photosynthetic apparatus, ensuring rapid recovery upon re‐illumination.

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“…The low amount of LHCX1 protein, for instance, explains limited NPQ capacity in a high latitude Phaeodactylum tricornutum ecotype isolated from a supralitoral rockpool (P.t.4) which experiences lower average light intensity, and less drastically fluctuating light conditions, compared to other ecotypes (Bailleul et al ). Whereas the LHCX1 gene is already maximally expressed in low light conditions, several other LHCX family members of both centric and pennate diatoms are highly and rapidly upregulated when exposed to high light (Nymark et al ; Park et al ; Zhu and Green ; Lepetit et al , in press) and other stressful environmental conditions that impair photosynthetic capacity (Taddei et al ). These proteins might either confer higher NPQ capacity by binding newly synthetized Dtx molecules and/or be involved in NPQs after prolonged high light exposure (Zhu and Green ; Lepetit et al , in press).…”

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confidence: 99%

Contrasting NPQ dynamics and xanthophyll cycling in a motile and a non‐motile intertidal benthic diatom

Blommaert

Huysman

Vyverman

et al. 2017

Limnology & Oceanography

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Diatoms living in intertidal sediments have to be able to rapidly adjust photosynthesis in response to often pronounced changes in light intensity during tidal cycles and changes in weather conditions. Strategies to deal with oversaturating light conditions, however, differ between growth forms. Motile epipelic diatoms can migrate to more optimal light conditions. In contrast, non-motile epipsammic diatoms appear to mainly rely on higher Non-Photochemical Quenching (NPQ) of chlorophyll a fluorescence to dissipate excess light energy, and this has been related to a larger pool of xanthophyll cycle (XC) pigments. We studied the effect of 1 h high Photosynthetically Available Radiation (PAR) (2000 lmol photons m 22 s 21 ) on the kinetics of the xanthophyll cycle and NPQ in both a motile diatom (Seminavis robusta) and a non-motile diatom (Opephora guenter-grassii) in an experimental set-up which did not allow for vertical migration. O. guenter-grassii could rapidly switch NPQ on and off by relying on fast XC kinetics. This species also demonstrated high de novo synthesis of xanthophylls within a relatively short period of time (1 h), including significant amounts of zeaxanthin, a feature not observed before in other diatoms. In contrast, S. robusta showed slower NPQ and associated XC kinetics, partly relying on NPQ conferred by de novo synthetized diatoxanthin molecules and synthesis of Light-Harvesting Complex X (LHCX) isoforms. Part of this observed NPQ increase, however, is sustained quenching (NPQs). Our data illustrate the high and diverse adaptive capacity of microalgal growth forms to maximize photosynthesis in dynamic light environments.

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Multisignal control of expression of the LHCX protein family in the marine diatomPhaeodactylum tricornutum

Abstract: HighlightMultiple stress signalling pathways regulate LHCX family gene expression in the diatom Phaeodactylum tricornutum to attune acclimation responses efficiently in highly variable ocean environments.

Cited by 97 publications

References 89 publications

The diatom Phaeodactylum tricornutum adjusts nonphotochemical fluorescence quenching capacity in response to dynamic light via fine‐tuned Lhcx and xanthophyll cycle pigment synthesis

The diatom Phaeodactylum tricornutum adjusts nonphotochemical fluorescence quenching capacity in response to dynamic light via fine‐tuned Lhcx and xanthophyll cycle pigment synthesis

Dark metabolism: a molecular insight into how the Antarctic sea‐ice diatom Fragilariopsis cylindrus survives long‐term darkness

Contrasting NPQ dynamics and xanthophyll cycling in a motile and a non‐motile intertidal benthic diatom

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