Phosphorus (P) Index assessment requires independent estimates of long-term average annual P loss from fields, representing multiple climatic scenarios, management practices, and landscape positions. Because currently available measured data are insufficient to evaluate P Index performance, calibrated and validated process-based models have been proposed as tools to generate the required data. The objectives of this research were to develop a regional parameterization for the Agricultural Policy Environmental eXtender (APEX) model to estimate edgeof-field runoff, sediment, and P losses in restricted-layer soils of Missouri and Kansas and to assess the performance of this parameterization using monitoring data from multiple sites in this region. Five site-specific calibrated models (SSCM) from within the region were used to develop a regionally calibrated model (RCM), which was further calibrated and validated with measured data. Performance of the RCM was similar to that of the SSCMs for runoff simulation and had Nash-Sutcliffe efficiency (NSE) > 0.72 and absolute percent bias (|PBIAS|) < 18% for both calibration and validation. The RCM could not simulate sediment loss (NSE < 0, |PBIAS| > 90%) and was particularly ineffective at simulating sediment loss from locations with small sediment loads. The RCM had acceptable performance for simulation of total P loss (NSE > 0.74, |PBIAS| < 30%) but underperformed the SSCMs. Total P-loss estimates should be used with caution due to poor simulation of sediment loss. Although we did not attain our goal of a robust regional parameterization of APEX for estimating sediment and total P losses, runoff estimates with the RCM were acceptable for P Index evaluation. T he Phosphorus (P) Index was developed as a tool to assess the risk of P loss from agricultural fields. Although this tool has been used to encourage the adoption of conservation practices and develop nutrient management plans, excess P losses from agricultural fields and associated water quality degradation persist ( Jarvie et al., 2013;Sharpley et al., 2015;USEPA, 2016). Due to the lack of water quality improvement and the disparity among state P Indices (Osmond et al., 2006), it has been proposed that the P Indices undergo evaluation to ensure accuracy in P-loss risk assessment (Sharpley et al., 2012).A wide variety of methods have been used to assess P Indices (Nelson and Shober, 2012); however, the ideal assessment would include comparison of P Index results to independently obtained quantitative estimates of long-term average annual P loss across a wide range of soils, topography, and management practices . This type of quantitative independent assessment requires long-term average annual estimates of P loss because the P Index is a generalized assessment of the average risk of P loss across an extended period, as opposed to an assessment of P loss for a specific year or weather sequence. Because measured edge-of-field P-loss data are highly dependent on the weather patterns during the years of data col...