Multispectral Opto-acoustic Tomography (MSOT) of the Brain and Glioblastoma Characterization

Burton, Neal C.; Patel, Manishkumar; Morscher, Stefan; Driessen, Wouter H. P.; Claussen, Jing; Bézière, Nicolas; Jetzfellner, Thomas; Taruttis, Adrian; Razansky, Daniel; Bednář, Bohumil; Ntziachristos, Vasilis

doi:10.1016/j.neuroimage.2012.09.053

Cited by 132 publications

(117 citation statements)

References 32 publications

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“…This imaging method is versatile, being able to provide multifaceted information: anatomical imaging based on endogenous tissue absorption, functional imaging of blood oxygenation, as well as molecular imaging based on absorption of exogenous contrast agents. Thus, it has been widely used in various applications, including imaging of the brain, [4][5][6] cancer, 7,8 cardiovasculature, 9,10 as well as the evaluation of gene delivery efficiency.…”

Section: Introductionmentioning

confidence: 99%

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Balasundaram¹,

Ho²,

Li³

et al. 2015

IJN

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Conjugated polymers (CPs) are upcoming optical contrast agents in view of their unique optical properties and versatile synthetic chemistry. Biofunctionalization of these polymer-based nanoparticles enables molecular imaging of biological processes. In this work, we propose the concept of using a biofunctionalized CP for noninvasive photoacoustic (PA) molecular imaging of breast cancer. In particular, after verifying the PA activity of a CP nanoparticle (CP dots) in phantoms and the targeting efficacy of a folate-functionalized version of the same (folate-CP dots) in vitro, we systemically administered the probe into a folate receptor-positive (FR+ve) MCF-7 breast cancer xenograft model to demonstrate the possible application of folate-CP dots for imaging FR+ve breast cancers in comparison to CP dots with no folate moieties. We observed a strong PA signal at the tumor site of folate-CP dots-administered mice as early as 1 hour after administration as a result of the active targeting of the folate-CP dots to the FR+ve tumor cells but a weak PA signal at the tumor site of CP-dots-administered mice as a result of the passive accumulation of the probe by enhanced permeability and retention effect. We also observed that folate-CP dots produced ~4-fold enhancement in the PA signal in the tumor, when compared to CP dots. These observations demonstrate the great potential of this active-targeting CP to be used as a contrast agent for molecular PA diagnostic imaging in various biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Balasundaram¹,

Ho²,

Li³

et al. 2015

IJN

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“…Their brains were excised and frozen after the experiments. To validate the lesion area, coronal brain slices were taken every 1 mm using a cryostat with two 10 μm sections being mounted on a metal slide [25]. No staining was applied here to get the frozen sections of a mouse brain.…”

Section: Histological Validationmentioning

confidence: 99%

“…Unlike horizontal plane PAT, cross-sectional PAT (csPAT) is often used to acquire images in coronal plane in both mouse and rat models [16,22]. It can be readily integrated into a multi-modal imaging system with ultrasound (US) and/or diffuse optical tomography (DOT) [19][20][21], offering more informed diagnosis of diseases.…”

Section: Introductionmentioning

confidence: 99%

Intracerebral haemorrhage-induced injury progression assessed by cross-sectional photoacoustic tomography

Yang

Zhang

et al. 2017

Biomed. Opt. Express

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Abstract:In this study, we in vivo examined injury progression after intracerebral haemorrhage (ICH) induced by collagenase in mice using cross-sectional photoacoustic tomography (csPAT). csPAT displayed high resolution with high sensitivity for ICH detection. The PAT images obtained showed high correlation with conventional histologic images. Quantitative analysis of the hematoma areas detected by csPAT showed high consistency with the neurologic deficit score (NDS). By utilizing the dual-wavelength method, the development of the hemoglobin area was monitored. Our results indicated that noninvasive csPAT can be used to track the dynamic progression of post-ICH, and to evaluate therapeutic interventions in preclinical ICH models. Wagner, "European Stroke Organisation (ESO) guidelines for the management of spontaneous intracerebral hemorrhage," Int. J. Stroke 9(7), 840-855 (2014). 2. C. J. van Asch, M. J. Luitse, G. J. Rinkel, I. van der Tweel, A. Algra, and C. J. Klijn, "Incidence, case fatality, and functional outcome of intracerebral haemorrhage over time, according to age, sex, and ethnic origin: a systematic review and meta-analysis," Lancet Neurol. 9(2), 167-176 (2010). 3. J. Yang, Q. Li, Z. Wang, C. Qi, X. Han, X. Lan, J. Wan, W. Wang, X. Zhao, Z. Hou, C. Gao, J. R. Carhuapoma, S. Mori, J. Zhang, and J. Wang, "Multimodality MRI assessment of grey and white matter injury and blood-brain barrier disruption after intracerebral haemorrhage in mice," Sci.

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“…Image Acquisition and Postprocessing MSOT imaging.-The two-dimensional (2D) static small-animal MSOT setup (inVision 256-TF; iThera Medical) used in this study has been previously described elsewhere (21). In brief, an illumination beam is generated by a wavelength-tunable (680-980 nm) opti cal parametric oscillator with pulse duration of less than 10 nsec (frame rate, 10 Hz).…”

Section: Molecular Imaging: In Vivo Detection Of Melanoma Lymph Node mentioning

confidence: 99%

Lymph Node Micrometastases and In-Transit Metastases from Melanoma: In Vivo Detection with Multispectral Optoacoustic Imaging in a Mouse Model

Neuschmelting¹,

Lockau

Ntziachristos

et al. 2016

Radiology

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Multispectral Opto-acoustic Tomography (MSOT) of the Brain and Glioblastoma Characterization

Cited by 132 publications

References 32 publications

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Intracerebral haemorrhage-induced injury progression assessed by cross-sectional photoacoustic tomography

Lymph Node Micrometastases and In-Transit Metastases from Melanoma: In Vivo Detection with Multispectral Optoacoustic Imaging in a Mouse Model

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