Multispectral optoacoustic and MRI coregistration for molecular imaging of orthotopic model of human glioblastoma

Attia, Amalina Binte Ebrahim; Ho, Chris Jun Hui; Chandrasekharan, Prashant; Balasundaram, Ghayathri; Tay, Hui Chien; Burton, Neal C.; Chuang, Kai‐Hsiang; Ntziachristos, Vasilis; Olivo, Malini

doi:10.1002/jbio.201500321

Cited by 38 publications

(32 citation statements)

References 36 publications

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“…Evans blue and Methylene blue have been used for PAI but are limited by their relatively blue-shifted absorption peak [ 5 ]. Other NIR dyes, not approved for human use, like IRDye800CW [ 29 ], AlexaFluor750 [ 30 ], ATTO740 [ 31 ], IR780 [ 32 ] and others [ 33 , 34 ] have been recently investigated as photoacoustic probes, either as is or chemically bound to biomolecules to generate targeted contrast agents. All these examples show the feasibility of using small NIR dyes for PAI and for tumor detection.…”

Section: Discussionmentioning

confidence: 99%

Photoacoustic imaging of integrin-overexpressing tumors using a novel ICG-based contrast agent in mice

Capozza

Blasi²,

Valbusa³

et al. 2018

Photoacoustics

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Section: Discussionmentioning

confidence: 99%

Photoacoustic imaging of integrin-overexpressing tumors using a novel ICG-based contrast agent in mice

Capozza

Blasi²,

Valbusa³

et al. 2018

Photoacoustics

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“…Recently, a wearable system has been developed that is capable of providing images of cerebral blood vessels noninvasively [ 199 ]. Moreover, PAI is a new strategy to visualize and study several brain disorders [ 200 ] including stroke [ 201 , 202 , 203 ], brain tumors [ 204 , 205 , 206 ]; cerebral edema [ 207 ], epilepsy [ 208 ]; traumatic brain injury [ 209 ] and inflammation [ 210 ].…”

Section: Photoacoustic Imaging (Pai)mentioning

confidence: 99%

Brain Vascular Imaging Techniques

Laviña

2016

IJMS

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Recent major improvements in a number of imaging techniques now allow for the study of the brain in ways that could not be considered previously. Researchers today have well-developed tools to specifically examine the dynamic nature of the blood vessels in the brain during development and adulthood; as well as to observe the vascular responses in disease situations in vivo. This review offers a concise summary and brief historical reference of different imaging techniques and how these tools can be applied to study the brain vasculature and the blood-brain barrier integrity in both healthy and disease states. Moreover, it offers an overview on available transgenic animal models to study vascular biology and a description of useful online brain atlases.

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“…Previously, we have demonstrated the utility of Multispectral Optoacoustic Tomography (MSOT) to investigate orthotopic brain tumors using exogenous contrast agent [20] . Burton et al [2] and Ni et al [21] have investigated the utility MSOT for identifying hypoxic areas in mouse brain.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive Anatomical and Functional Imaging of Orthotopic Glioblastoma Development and Therapy using Multispectral Optoacoustic Tomography

Balasundaram

Ding

et al. 2018

Translational Oncology

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PURPOSE: Here we demonstrate the potential of multispectral optoacoustic tomography (MSOT), a new non-invasive structural and functional imaging modality, to track the growth and changes in blood oxygen saturation (sO2) in orthotopic glioblastoma (GBMs) and the surrounding brain tissues upon administration of a vascular disruptive agent (VDA). METHODS: Nude mice injected with U87MG tumor cells were longitudinally monitored for the development of orthotopic GBMs up to 15 days and observed for changes in sO2 upon administration of combretastatin A4 phosphate (CA4P, 30 mg/kg), an FDA approved VDA for treating solid tumors. We employed a newly-developed non-negative constrained approach for combined MSOT image reconstruction and unmixing in order to quantitatively map sO2 in whole mouse brains. RESULTS: Upon longitudinal monitoring, tumors could be detected in mouse brains using single-wavelength data as early as 6 days post tumor cell inoculation. Fifteen days post-inoculation, tumors had higher sO2 of 63 ± 11% (n = 5, P < .05) against 48 ± 7% in the corresponding contralateral brain, indicating their hyperoxic status. In a different set of animals, 42 days post-inoculation, tumors had lower sO2 of 42 ± 5% against 49 ± 4% (n = 3, P < .05) in the contralateral side, indicating their hypoxic status. Upon CA4P administration, sO2 in 15 days post-inoculation tumors dropped from 61 ± 9% to 36 ± 1% (n = 4, P < .01) within one hour, then reverted to pre CA4P treatment values (63 ± 6%) and remained constant until the last observation time point of 6 hours. CONCLUSION: With the help of advanced post processing algorithms, MSOT was capable of monitoring the tumor growth and assessing hemodynamic changes upon administration of VDAs in orthotopic GBMs.

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Multispectral optoacoustic and MRI coregistration for molecular imaging of orthotopic model of human glioblastoma

Cited by 38 publications

References 36 publications

Photoacoustic imaging of integrin-overexpressing tumors using a novel ICG-based contrast agent in mice

Photoacoustic imaging of integrin-overexpressing tumors using a novel ICG-based contrast agent in mice

Brain Vascular Imaging Techniques

Noninvasive Anatomical and Functional Imaging of Orthotopic Glioblastoma Development and Therapy using Multispectral Optoacoustic Tomography

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