Multispectral optoacoustic tomography (MSOT): Monitoring neurovascular changes in a mouse repetitive traumatic brain injury model

Penn, Courtney A.; Katnik, Chris; Cuevas, J.; Mohapatra, Shyam S.; Mohapatra, Subhra

doi:10.1016/j.jneumeth.2023.109876

Cited by 5 publications

(4 citation statements)

References 87 publications

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“…When short laser pulses (typically nanoseconds) are absorbed by molecules in tissue, the resulting rapid rise in temperature causes thermoelastic expansion and contraction and generates acoustic waves, which an ultrasound transducer can detect (Figure 3a). 41,42 Therefore, PAI combines the high contrast of optical absorption with the deep penetration of ultrasound detection because acoustic waves have 3 orders of magnitude weaker scattering than light. 43 The principle and theory of photoacoustic imaging has been extensively reviewed in previous publications.…”

Section: ■ Functional Near Infrared Spectroscopymentioning

confidence: 99%

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Quantitative Optical Imaging of Oxygen in Brain Vasculature

Rathbone,

2024

J. Phys. Chem. B

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The intimate relationship between neuronal activity and cerebral oxygenation underpins fundamental brain functions like cognition, sensation, and motor control. Optical imaging offers a noninvasive approach to assess brain oxygenation and often serves as an indirect proxy for neuronal activity. However, deciphering neurovascular coupling�the intricate interplay between neuronal activity, blood flow, and oxygen delivery�necessitates independent, high spatial resolution, and high temporal resolution measurements of both microvasculature oxygenation and neuronal activation. This Perspective examines the established optical techniques employed for brain oxygen imaging, specifically functional nearinfrared spectroscopy, photoacoustic imaging, optical coherence tomography, and two-photon phosphorescent lifetime microscopy, highlighting their fundamental principles, strengths, and limitations. Several other emerging optical techniques are also introduced. Finally, we discuss key technological challenges and future directions for quantitative optical oxygen imaging, paving the way for a deeper understanding of oxygen metabolism in the brain.

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Section: ■ Functional Near Infrared Spectroscopymentioning

confidence: 99%

Section: Functional Near Infrared Spectroscopymentioning

confidence: 99%

Quantitative Optical Imaging of Oxygen in Brain Vasculature

Rathbone,

2024

J. Phys. Chem. B

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“…[5] Using the differences in absorbance profiles of oxygenated and deoxygenated hemoglobin, it is possible to glean information regarding tumor size and location. [6,7] PAI applications extend beyond cancer detection, encompassing fields such as neuroscience, [8,9] cardiovascular imaging, [5,10,11] and functional imaging of organs, [12,13] further highlighting its emerging role in biomedical research and clinical diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Xanthene‐Based Dyes for Photoacoustic Imaging and their Use as Analyte‐Responsive Probes

Brøndsted,

Stains

2024

Chemistry A European J

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Developing imaging tools that can report on the presence of disease‐relevant analytes in multicellular organisms can provide insight into fundamental disease mechanisms as well as provide diagnostic tools for the clinic. Photoacoustic imaging (PAI) is a light‐in, sound‐out imaging technique that allows for high resolution, deep‐tissue imaging with applications in pre‐clinical and point‐of‐care settings. The continued development of near‐infrared (NIR) absorbing small‐molecule dyes promises to improve the capabilities of this emerging imaging modality. For example, new dye scaffolds bearing chemoselective functionalities are enabling the detection and quantification of disease‐relevant analytes through activity‐based sensing (ABS) approaches. Recently described strategies to engineer NIR absorbing xanthenes have enabled development of analyte‐responsive PAI probes using this classic dye scaffold. Herein, we present current strategies for red‐shifting the spectral properties of xanthenes via bridging heteroatom or auxochrome modifications. Additionally, we explore how these strategies, coupled with chemoselective spiroring‐opening approaches, have been employed to create ABS probes for in vivo detection of hypochlorous acid, nitric oxide, copper (II), human NAD(P)H: quinone oxidoreductase isozyme 1, and carbon monoxide. Given the versatility of the xanthene scaffold, we anticipate continued growth and development of analyte‐responsive PAI imaging probes based on this dye class.

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“…By using sets of different wavelengths, technics like multispectral optoacoustic tomography (MSOT) may provide information about various endogenous molecules such as deoxygenated and oxygenated hemoglobin, melanin, collagen, and lipids [1, 3, 4]. The high resolution and deep penetration of MSOT enables its clinical application to a broad disease spectrum, including inflammatory bowel disease [5–7], breast cancer [8], muscular degeneration [9–12], cardiovascular diseases [2, 6, 13], and functional liver assessment [14, 15].…”

Section: Introductionmentioning

confidence: 99%

Teachability of multispectral optoacoustic tomography

Li,

Gröhl,

Haney

et al. 2024

Journal of Biophotonics

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To date, the appropriate training required for the reproducible operation of multispectral optoacoustic tomography (MSOT) is poorly discussed. Therefore, the aim of this study was to assess the teachability of MSOT imaging. Five operators (two experienced and three inexperienced) performed repositioning imaging experiments. The inexperienced received the following introductions: personal supervision, video meeting, or printed introduction. The task was to image the exact same position on the calf muscle for seven times on five volunteers in two rounds of investigations. In the first session, operators used ultrasound guidance during measurements while using only photoacoustic data in the second session. The performance comparison was carried out with full‐reference image quality measures to quantitatively assess the difference between repeated scans. The study demonstrates that given a personal supervision and hybrid ultrasound real‐time imaging in MSOT measurements, inexperienced operators are able to achieve the same level as experienced operators in terms of repositioning accuracy.

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Multispectral optoacoustic tomography (MSOT): Monitoring neurovascular changes in a mouse repetitive traumatic brain injury model

Cited by 5 publications

References 87 publications

Quantitative Optical Imaging of Oxygen in Brain Vasculature

Quantitative Optical Imaging of Oxygen in Brain Vasculature

Xanthene‐Based Dyes for Photoacoustic Imaging and their Use as Analyte‐Responsive Probes

Teachability of multispectral optoacoustic tomography

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