Multispectral raster-scanning optoacoustic mesoscopy differentiate lesional from non-lesional atopic dermatitis skin using structural and functional imaging markers

Li, Xiuting; Moothanchery, Mohesh; Kwa, Cheng Yi; Tan, Wan Ling; Yew, Yik Weng; Thng, Steven Tien Guan; Dinish, U. S.; Attia, Amalina Binte Ebrahim; Olivo, Malini

doi:10.1016/j.pacs.2022.100399

Cited by 11 publications

(6 citation statements)

References 40 publications

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“…[282][283][284] In recent years, there has been a growing interest among various companies in the development and commercialization of PAI systems for medical applications. 145,285 Among them are iThera Medical GmbH, 105,130,180,[286][287][288][289][290][291][292][293][294][295][296][297] FUJIFILM VisualSonics, 135,171,187,298 ENDRA Life Sciences Inc., [299][300][301][302][303] TomoWave Laboratories, [304][305][306][307] and Seno Medical Instruments. 308,309 Although there exist commercial systems for skin imaging, there has not been any customized clinical system for melanoma imaging applications.…”

Section: Discussionmentioning

confidence: 99%

Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review

Fakhoury,

Lara,

Manwar

et al. 2024

J. Biomed. Opt.

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Significance: Cutaneous melanoma (CM) has a high morbidity and mortality rate, but it can be cured if the primary lesion is detected and treated at an early stage. Imaging techniques such as photoacoustic (PA) imaging (PAI) have been studied and implemented to aid in the detection and diagnosis of CM.Aim: Provide an overview of different PAI systems and applications for the study of CM, including the determination of tumor depth/thickness, cancer-related angiogenesis, metastases to lymph nodes, circulating tumor cells (CTCs), virtual histology, and studies using exogenous contrast agents.Approach: A systematic review and classification of different PAI configurations was conducted based on their specific applications for melanoma detection. This review encompasses animal and preclinical studies, offering insights into the future potential of PAI in melanoma diagnosis in the clinic.Results: PAI holds great clinical potential as a noninvasive technique for melanoma detection and disease management. PA microscopy has predominantly been used to image and study angiogenesis surrounding tumors and provide information on tumor characteristics. Additionally, PA tomography, with its increased penetration depth, has demonstrated its ability to assess melanoma thickness. Both modalities have shown promise in detecting metastases to lymph nodes and CTCs, and an alloptical implementation has been developed to perform virtual histology analyses. Animal and human studies have successfully shown the capability of PAI to detect, visualize, classify, and stage CM.Conclusions: PAI is a promising technique for assessing the status of the skin without a surgical procedure. The capability of the modality to image microvasculature, visualize tumor boundaries, detect metastases in lymph nodes, perform fast and label-free histology, and identify CTCs could aid in the early diagnosis and classification of CM, including determination of metastatic status. In addition, it could be useful for monitoring treatment efficacy noninvasively.

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Section: Discussionmentioning

confidence: 99%

Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review

Fakhoury,

Lara,

Manwar

et al. 2024

J. Biomed. Opt.

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“…The laser energy measured on the skin surface was 10 mJ/cm2, which was less than the maximum permissible skin exposure (20 mJ/cm2) specified by the American National Standards Institute. In addition, the biosafety of our experimental procedure involving laser irradiation after topical steroid treatment has been validated by clinical studies of patients applying topical steroid treatment using clinical PA imaging systems 52 , 53 …”

Section: Methodsmentioning

confidence: 99%

“…In addition, the biosafety of our experimental procedure involving laser irradiation after topical steroid treatment has been validated by clinical studies of patients applying topical steroid treatment using clinical PA imaging systems. 52,53 3 Results and Discussion…”

Section: Experimental Procedures For In Vivo Pa Monitoringmentioning

confidence: 99%

In vivo quantitative photoacoustic monitoring of corticosteroid-induced vasoconstriction

et al. 2023

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Significance: Corticosteroids-commonly prescribed medications for skin diseases-inhibit the secretion of vasodilators, such as prostaglandin, thereby exerting anti-inflammatory action by constricting capillaries in the dermis. The effectiveness of corticosteroids is determined by the degree of vasoconstriction followed by skin whitening, namely, the blanching effect. However, the current method of observing the blanching effect indirectly evaluates the effects of corticosteroids.Aim: In this study, we employed optical-resolution photoacoustic (PA) microscopy (OR-PAM) to directly visualize the blood vessels and quantitatively evaluate vasoconstriction.Approach: Using OR-PAM, the vascular density in mice skin was monitored for 60 min after performing each experimental procedure for four groups, and the vasoconstriction was quantified. Volumetric PA data were segmented into the papillary dermis, reticular dermis, and hypodermis based on the vascular characteristics obtained through OR-PAM. The vasoconstrictive effect of each skin layer was quantified according to the dermatological treatment method.Results: In the case of corticosteroid topical application, vasoconstriction was observed in the papillary (56.4 AE 10.9%) and reticular (45.1 AE 4.71%) dermis. For corticosteroid subcutaneous injection, constriction was observed solely in the reticular (49.5 AE 9.35%) dermis. In contrast, no vasoconstrictions were observed with nonsteroidal topical application. Conclusions:Our results indicate that OR-PAM can quantitatively monitor the vasoconstriction induced by corticosteroids, thereby validating OR-PAMs potential as a practical evaluation tool for predicting the effectiveness of corticosteroids in dermatology.

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“…Using wavelength-tunable high repetition rate laser, PA images of a layer of tissue acquired at series of distinct wavelengths can be spectrally unmixed according to its chromophores, such as melanin, oxy-hemoglobin, and deoxy-hemoglobin, and the composition ratio between two hemoglobin distribution may spatially encode oxygen saturation map. In a clinical application, Li et al used multispectral RSOM to track morphological and oxygenation changes in severe atopic dermatitis [ 147 ]. The clinical applicability of RSOM was demonstrated by comparative analysis of changes in the SO 2 and epidermal thickness of skin microvessels before and after treatment.…”

Section: Advances In Functional Photoacoustic Imaging At Different Sc...mentioning

confidence: 99%

Functional photoacoustic imaging: from nano- and micro- to macro-scale

2023

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Functional photoacoustic imaging is a promising biological imaging technique that offers such unique benefits as scalable resolution and imaging depth, as well as the ability to provide functional information. At nanoscale, photoacoustic imaging has provided super-resolution images of the surface light absorption characteristics of materials and of single organelles in cells. At the microscopic and macroscopic scales. photoacoustic imaging techniques have precisely measured and quantified various physiological parameters, such as oxygen saturation, vessel morphology, blood flow, and the metabolic rate of oxygen, in both human and animal subjects. This comprehensive review provides an overview of functional photoacoustic imaging across multiple scales, from nano to macro, and highlights recent advances in technology developments and applications. Finally, the review surveys the future prospects of functional photoacoustic imaging in the biomedical field.

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Multispectral raster-scanning optoacoustic mesoscopy differentiate lesional from non-lesional atopic dermatitis skin using structural and functional imaging markers

Cited by 11 publications

References 40 publications

Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review

Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review

In vivo quantitative photoacoustic monitoring of corticosteroid-induced vasoconstriction

Functional photoacoustic imaging: from nano- and micro- to macro-scale

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