Multistate outbreak of <i>Listeria monocytogenes</i> infections linked to whole apples used in commercially produced, prepackaged caramel apples: United States, 2014–2015

Angelo, Kristina M.; Conrad, Amanda; Saupe, Amy; Dragoo, H.; West, Nicole; Sorenson, Alida; Barnes, Amber N.; Doyle, Matthew; Beal, Jennifer; Jackson, Kelly A.; Stroika, Steven; Tarr, Cheryl L.; Kučerová, Zuzana; Lance, Susan; Gould, L. Hannah; Wise, Matthew E.; Jackson, Brendan R

doi:10.1017/s0950268816003083

Cited by 164 publications

(100 citation statements)

References 23 publications

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“…Typically, pregnant women are advised to avoid consumption of foods at high risk of Listeria contamination. However, a series of deadly Listeria outbreaks in fresh produce, fruit, and other foods traditionally at low risk of Listeria contamination, highlight the need for alternative and novel approaches to safeguarding the food supply (32)(33)(34).…”

Section: Therapeutic Potential Of Listeria-specific V H Hmentioning

confidence: 99%

Structural basis of VHH-mediated neutralization of the food-borne pathogen Listeria monocytogenes

King

Huh

Shenai

et al. 2018

Journal of Biological Chemistry

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causes listeriosis, a potentially fatal food-borne disease. The condition is especially harmful to pregnant women. outbreaks can originate from diverse foods, highlighting the need for novel strategies to improve food safety. The first step in invasion is internalization of the bacteria, which is mediated by the interaction of the internalin family of virulence factors with host cell receptors. A crucial interaction for invasion of the placenta, and thus a target for therapeutic intervention, is between internalin B (InlB) and the receptor c-Met. Single-domain antibodies (VH, also called nanobodies, or sdAbs) from camel heavy-chain antibodies are a novel solution for preventing infections. The VH R303, R330, and R326 all bind InlB with high affinity; however, the molecular mechanism behind their mode of action was unknown. We demonstrate that despite a high degree of sequence and structural diversity, the VH bind a single epitope on InlB. A combination of gentamicin protection assays and florescent microscopy establish that InlB-specific VH inhibit invasion of HeLa cells. A high-resolution X-ray structure of VH R303 in complex with InlB showed that the VH binds at the c-Met interaction site on InlB, thereby acting as a competitive inhibitor preventing bacterial invasion. These results point to the potential of VH as a novel class of therapeutics for the prevention of listeriosis.

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Section: Therapeutic Potential Of Listeria-specific V H Hmentioning

confidence: 99%

Structural basis of VHH-mediated neutralization of the food-borne pathogen Listeria monocytogenes

King

Huh

Shenai

et al. 2018

Journal of Biological Chemistry

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“…The persistence of certain subtypes of L. monocytogenes in food processing facilities or on equipment has been reported for up to 10 years and linked to food contamination from farm to fork [5]. The persistence of such strains in the food system has also been observed to play a significant role in listeriosis outbreaks during the last decades [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Dynamics of mobile genetic elements of Listeria monocytogenes persisting in ready-to-eat seafood processing plants in France

et al. 2020

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Background: Listeria monocytogenes Clonal Complexes (CCs) have been epidemiologically associated with foods, especially ready-to-eat (RTE) products for which the most likely source of contamination depends on the occurrence of persisting clones in food-processing environments (FPEs). As the ability of L. monocytogenes to adapt to environmental stressors met in the food chain challenges the efforts to its eradication from FPEs, the threat of persistent strains to the food industry and public health authorities continues to rise. In this study, 94 food and FPEs L. monocytogenes isolates, representing persistent subtypes contaminating three French seafood facilities over 2-6 years, were whole-genome sequenced to characterize their genetic diversity and determine the biomarkers associated with long-term survival in FPEs. Results: Food and FPEs isolates belonged to five CCs, comprising long-term intra-and inter-plant persisting clones. Mobile genetic elements (MGEs) such as plasmids, prophages and transposons were highly conserved within CCs, some of which harboured genes for resistance to chemical compounds and biocides used in the processing plants. Some of these genes were found in a 90.8 kbp plasmid, predicted to be" mobilizable", identical in isolates from CC204 and CC155, and highly similar to an 81.6 kbp plasmid from isolates belonging to CC7. These similarities suggest horizontal transfer between isolates, accompanied by deletion and homologous recombination in isolates from CC7. Prophage profiles characterized persistent clonal strains and several prophage-loci were plant-associated. Notably, a persistent clone from CC101 harboured a novel 31.5 kbp genomic island that we named Listeria genomic island 3 (LGI3), composed by plant-associated loci and chromosomally integrating cadmium-resistance determinants cadA1C. Conclusions: Genome-wide analysis indicated that inter-and intra-plant persisting clones harbour conserved MGEs, likely acquired in FPEs and maintained by selective pressures. The presence of closely related plasmids in L. monocytogenes CCs supports the hypothesis of horizontal gene transfer conferring enhanced survival to FPEassociated stressors, especially in hard-to-clean harbourage sites. Investigating the MGEs evolutionary and transmission dynamics provides additional resolution to trace-back potentially persistent clones. The biomarkers herein discovered provide new tools for better designing effective strategies for the removal or reduction of resident L. monocytogenes in FPEs to prevent contamination of RTE seafood.

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“…An outbreak lasting from October 2014 to February 2015 in the United States was caused by L. monocytogenes contaminated whole apples. 71 This outbreak caused 35 illnesses across 12 states; 34 of the affected were hospitalized and seven died. A major outbreak occurred due to the consumption of contaminated cantaloupes originating from Jensen Farms in Colorado, in 2011 in the United States was the cause of 147 illnesses, 33 deaths, and one miscarriage.…”

Section: Listeria Monocytogenesmentioning

confidence: 99%

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2019

MOJFPT

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This article provides a comprehensive literature review of the growing significance that foodborne pathogens have on the produce supply and the steps that are taken to reduce them. The numbers of illnesses that are caused by contaminated produce are rising steadily. Three of the most common pathogens that have been seen to cause significant illness in humans through produce are Escherichia coli, Salmonella spp. and Listeria monocytogenes. The antimicrobial interventions, practices and antimicrobials that are currently the most commonly used by the food industry are examined. The literature shows that these antimicrobials currently in use have significant drawbacks when it comes to their role in the produce industry. Sensitivities to temperature and organic load and the production of harmful by products are some of these drawbacks. There is a need for a new antimicrobial that can be used in the produce processing industry. One that maintains its efficacy through the temperature, pH, and organic load changes that are experienced during produce processing. Sodium acid sulfate is a potential contender for this role. It has not previously been used or extensively studied as an antimicrobial, but is already deemed generally recognized as safe (GRAS) product by the Food and Drug Administration (FDA). The use of an antimicrobial that can withstand the varying conditions that the produce industry constantly has is imperative to reducing the number of foodborne illness caused every year by contaminated produce.

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Multistate outbreak of Listeria monocytogenes infections linked to whole apples used in commercially produced, prepackaged caramel apples: United States, 2014–2015

Cited by 164 publications

References 23 publications

Structural basis of VHH-mediated neutralization of the food-borne pathogen Listeria monocytogenes

Structural basis of VHH-mediated neutralization of the food-borne pathogen Listeria monocytogenes

Dynamics of mobile genetic elements of Listeria monocytogenes persisting in ready-to-eat seafood processing plants in France

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