Multistep centrifugal consolidation method for characterization of filterability of aggregated concentrated suspensions

Loginov, Maksym; Zierau, Anne; Kavianpour, Davood; Lerche, D.; Vorobiev, Eugène; Gésan-Guiziou, Geneviève; Mahnič-Kalamiza, Samo; Sobisch, Titus

doi:10.1016/j.seppur.2017.03.067

Cited by 11 publications

(5 citation statements)

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“…What could be the next steps in cuvette-based analytical photocentrifugation? It would be beneficial to increase the rotor speed to reduce the measurement time and to increase the pressure load for consolidation and filtration experiments (Loginov et al, 2017b). Higher flexibility with respect to the multi-wavelength approach would broaden application fields and allow 2-dimensional particle characterization.…”

Section: Discussionmentioning

confidence: 99%

“…The instability or separation index (Detloff et al, 2014), integral transmission and front tracking (Lerche et al 2001), (Sobisch et al, 2010) or first derivative (Wiese, 2010) allow application-specific comparison and ranking of differently designed formulations and products and identification of destabilization phenomena as well as prediction of shelf life (Badolato et al, 2008), , (Takeda, 2012), (Staudinger et al, 2014), , (Cai et al, 2018). Nowadays, the use of AC-L gains also large interest in characterizing highly concentrated dispersions, especially in the field of centrifugal filtration and sediment consolidation (Usher et al, 2013), (Cao et al, 2014), (Loginov et al, 2017a(Loginov et al, , 2017b as well as mechanical properties (rigidity, deformability) of e.g. hydrocolloid beads (Schuldt et al, 2018).…”

Section: Figmentioning

confidence: 99%

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Comprehensive Characterization of Nano- and Microparticles by In-Situ Visualization of Particle Movement Using Advanced Sedimentation Techniques

Lerche¹

2019

KONA

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The state of a suspension is crucial with regard to processing pathways, functionality and performance of the end product. In the past decade, substantial progress has been made in designing highly specialized and functionalized particles. In current particle technology, besides classic particle properties such as particle size distribution, shape and density, surface properties play an essential role for processing, product specification and use. For example, in medical therapy, analytical diagnostic applications, as well as in separation processing and harvesting of high-valued materials, magnetic micro-and nanoparticles play an increasing role. In addition to traditional parameters such as size, the particle magnetization has to be quantified here. Sedimentation techniques have been used for hundreds of years to determine the geometrical characteristics of dispersed particles. Numerous national and international standards regarding these techniques have been published. Mainly due to the fast growing market share of laser scattering techniques over the past two decades, most customers these days are not aware of some advantageous features of particle characterization via a firstprinciple fractionating approach such as sedimentation. This is unfortunate as sedimentation techniques have made huge technological leaps forward regarding electronics, sensors and computing abilities. This paper aims to give a short review about different cumulative and incremental sedimentation approaches to measure the particle size distribution. It focuses mainly on the in-situ visualization (STEP-Technology ® ) of particle migration in gravitational and centrifugal fields. It describes the basics of the new multi-sample measuring approaches to quantify the separation kinetics by spatial and time-resolved particle concentration over the entire sample height. Based on these data, the sedimentation velocity and particle size distribution are elucidated and estimates of accuracy, precision and experimental uncertainties are discussed. Multi-wavelength approaches, correction of higher concentration, and the influence of rheological behavior of continuous phase will also be discussed. Applications beyond the traditional scope of sedimentation analysis are presented. This concerns the in-situ determination of hydrodynamic particle density and of magnetophoretic velocity distributions for magnetic particulate objects.

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Section: Discussionmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Comprehensive Characterization of Nano- and Microparticles by In-Situ Visualization of Particle Movement Using Advanced Sedimentation Techniques

Lerche¹

2019

KONA

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“…For solid particles, the particle volume fraction Φ can be calculated considering the solid weight fraction of the suspension c s , the particle density ρ p and liquid density ρ l [15]. Living cells, however, contain intracellular water, which has to be considered.…”

Section: Methodsmentioning

confidence: 99%

“…This approach is a complex procedure with one sample at a time, and an alternative screening method would be beneficial. We therefore developed a method able to assess compressibility as well as reversibility of compression of biological particulate matter mixed with biogenic macromolecules, based on the previously described multistep centrifugal consolidation method proposed by Loginov, et al [15]. As a model particle, yeast cells were used, an established model foulant also used in other microfiltration studies [16][17][18][19], which are also of economic relevance in biotechnological applications, such as production of biopharmaceuticals and biorefinery.…”

Section: Introductionmentioning

confidence: 99%

Determination of Compressibility and Relaxation Behavior of Yeast Cell Sediments by Analytical Centrifugation and Comparison with Deposit Formation on Membrane Surfaces

Weinberger

Kulozik

2022

Membranes

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Separation of cells from produced biomolecules is a challenging task in many biotechnological downstream operations due to deposit formation of the retained cells, affecting permeation of the target product. Compression and relaxation behavior of cell deposits formed during filtration are important factors affecting operational performance. The determination of these factors by flux or pressure stepping experiments is time- and labor-intensive. In this work, we propose a screening method by analytical centrifugation, which is capable of detecting small differences in compression and relaxation behavior induced by milieu changes, using a model system comprised of washed and unwashed yeast cells in the presence or absence of bovine serum albumin as a model target protein. The main effects observed were firstly the impact of pH value, affecting interaction of bovine serum albumin and yeast cells especially close to the isoelectric point, and secondly the effect of washing the yeast cells prior to analysis, where the presence of extracellular polymeric substances led to higher compressibility of the deposited cells. By comparing and validating the obtained results with dead-end filtration trials, the stabilizing role of bovine serum albumin in deposits formed at low pH values due to interactions with the yeast cells was underlined.

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“…The cumulative velocity distributions were always determined in a region of the transmission profiles where effects of the formed sediment on the settling velocity could be excluded. A detailed description of the applied analytic method is given in literature , . This analysis was performed at constant centrifugal force up to 2300 g .…”

Section: Methodsmentioning

confidence: 99%

Flocculant Screening Method at Lab Scale for Application in Disc Stack Centrifuges with Hermetic Design

2018

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Chemicals produced using biotechnological methods like fermentation processes are obtained as complex diluted aqueous mixture, which still contains the production organism. Centrifugation is a commonly used technology for biomass separation. By flocculation, the settling velocity of microorganisms can be increased. Here, a laboratory flocculant screening method tailored for the separation of flocculated biomass in a fully hermetic disc stack centrifuge is described. The specific requirements of this process, namely, floc formation, floc stability, sliding behavior in the disc stack, and flowability of the sludge, were transferred to lab scale and validated in pilot tests. The qualitative results of the laboratory screening were in agreement with the processes at industrial scale.

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Multistep centrifugal consolidation method for characterization of filterability of aggregated concentrated suspensions

Cited by 11 publications

References 40 publications

Comprehensive Characterization of Nano- and Microparticles by In-Situ Visualization of Particle Movement Using Advanced Sedimentation Techniques

Comprehensive Characterization of Nano- and Microparticles by In-Situ Visualization of Particle Movement Using Advanced Sedimentation Techniques

Determination of Compressibility and Relaxation Behavior of Yeast Cell Sediments by Analytical Centrifugation and Comparison with Deposit Formation on Membrane Surfaces

Flocculant Screening Method at Lab Scale for Application in Disc Stack Centrifuges with Hermetic Design

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