Multitarget FISH and LOH Analyses at Chromosome 3p in Non-Small Cell Lung Cancer and Adjacent Bronchial Epithelium

Abstract

Multitarget fluorescence in situ hybridization (FISH; LAVysion, Vysis, Downers Grove, IL) targeting chromosomes 6p11-q11, 7p12, 8q24, and 5p15.2 was compared with results of microsatellite studies at chromosome 3p to identify molecular changes associated with tobacco use and tumor development in non-small cell lung cancer (NSCLC). Analyses were performed on 26 NSCLCs and matched benign bronchial epithelium; samples from 10 patients without NSCLC served as control samples. Significant molecular differences between tumor tissue and corresponding benign bronchi were found using FISH (P = .001) and loss of heterozygosity (LOH) analysis (P = .031). Bronchial epithelium from patients with NSCLC was genetically different from epithelium from patients without NSCLC in FISH analysis (P = .025). Receiver operating characteristic curve analysis revealed an optimal cutoff value of 5% atypical cells for bronchial epithelium. There was no statistical correlation with the patient's smoking history, and LOH analysis of bronchi did not differentiate between patients with and without NSCLC. Multicolor FISH analysis is able to detect a tumor-associated molecular field effect in bronchi adjacent to NSCLC.

Comparison of allelic losses in chondroblastoma and primary chondrosarcoma of bone and correlation with fluorescence in situ hybridization analysis

“…This finding was validated by correlation with FISH results. Although chromosome loss can be difficult to identify with FISH on paraffin sections as a result of nuclear truncation and overlapping nuclei, a good, but not perfect, correlation has been observed in prior studies [35,36]. Given the loss of 17p13 in the recurrent but not in the primary CSs of the present and previous prior studies [28][29][30]33,34], it is possible that p53 mutations may represent a late event in the progression of CSs.…”

Expression of Telomere-Associated Genes as Prognostic Markers for Overall Survival in Patients with Non–Small Cell Lung Cancer

“…However, some studies have raised the question that whether normal adjacent tissues can truly represent normal tissues in these type of studies due to a ''field'' effect (35,36). They suggested that, in some cases, tissues adjacent to cancer, although appearing morphologically normal, may contain genetic changes associated with cancer (35,36), which might even influence gene expression (35). Therefore, difference in baseline or normal tissue collection might also contribute to controversial reports on telomere-associated protein expression in cancer.…”

Refined mapping of loss of heterozygosity on 1q31.1-32.1 in sporadic colorectal carcinoma

“…LOH is common to all human solid tumors and allows expressivity of recessive loss of function mutations of tumor suppressor genes. Therefore, detection of recurrent LOH in a chromosome region is now considered critical evidence of localization of tumor suppressor genes [3,[25][26][27][28] . In the previous study, initial LOH scanning was carried out in 83 sporadic colorectal carcinoma samples with 21 highly polymorphic markers on chromosome 1.…”