Multitherapeutic Efficacy of Curly Kale Extract Fabricated Biogenic Silver Nanoparticles

Abstract

Purpose Due to the biomedical applications universally, the Ag nanoparticles are one of the most commonly investigated nanoparticles (NPs). Curly kale (BroL) leaves contain numerous beneficial nutrients and phytochemicals. The aim of the current study is the fabrication of the Ag nanoparticles using the extracts of curly kale and to investigate their biological potentials. Methods The characterization of the generated BroLAgNPs was done through UV-Vis spectro study, Fourier-transform infrared spectro study, scanning electron microscope analysis, energy-dispersive X-ray study, distribution of size and zeta potential investigation, and X-ray powder diffraction study, and their biological effects were evaluated by antidiabetic, antioxidant, antibacterial and cytotoxicity effect. Results BroL-Ag nanoparticle displayed surface plasmon resonance at 432 nm. The Zeta potential of BroL (−26.6) AgNPs displayed a highly negative charge. In antidiabetic assay, BroL-AgNPs was highly effective with IC 50 value 2.29 µg/mL at 1.0 µg/mL concentration. In cytotoxicity assay, BroL-AgNPs displayed strong activity at 10.0 µg/mL concentration. It showed inhibitory action against three food-borne pathogenic bacteria (9.29–11.44 mm inhibition zone) and displayed moderate antioxidant potential. Conclusion This study as a whole report an eco-friendly green synthesis of AgNPs using leafy vegetable aqueous extract and its multi-biological effects which could serve as a promising candidate in pharmacological and related industries.

Investigation of Antimicrobial Activity and Biocompatibility of Biogenic Silver Nanoparticles Synthesized using Syzigyum cymosum Extract

“…Hence, pH 8 solution is highly desirable in order to synthesize small-size nanoparticles. Additionally, our as-prepared bAgNPs demonstrated similar SPR (surface plasmon resonance) bands (ranging from 400 to 450 nm) with those of other AgNPs reported in the literature. , However, the particle size was different, which can be attributed to the presence of different plant secondary metabolites in S. cymosum extract .…”

“…Additionally, our as-prepared bAgNPs demonstrated similar SPR (surface plasmon resonance) bands (ranging from 400 to 450 nm) with those of other AgNPs reported in the literature. 37 , 38 However, the particle size was different, which can be attributed to the presence of different plant secondary metabolites in S. cymosum extract. 39 The average size of bAgNPs synthesized at pH 8 was smaller than most of the bAgNPs reported in the literature.…”

Synthesis of Biogenic Gold Nanoparticles by Using Sericin Protein from Bombyx mori Silk Cocoon and Investigation of Its Wound Healing, Antioxidant, and Antibacterial Potentials

“… 47 The high negative charge zeta potential of the SSP-AuNPs represents the long period stability of the synthesized NPs with better colloidal nature. 37 , 48 Further, it is said that the high positive or negative values of the zeta potential showed a tendency to repel each other among the nanoparticles and thus reduce the accumulation of the NPs among each other. 49 Here, the polydispersed nature of the SSP-AuNPs is mainly because of the high negative zeta potential which averts the aggregation of the NPs among each other, resulting in well dispersed and stable materials.…”

“…The ABTS radical scavenging activity of SSP-AuNPs was determined as per the standard procedure of Das et al 37 Before the experiment, stock solutions of 7.4 mM ABTS and 2.6 mM of potassium persulfate were prepared and mixed equally and kept in dark for 12 h. For the experiment, briefly, 30 μL of different concentrations of the SSP-AuNPs (25, 50, and 100 μg/mL) and the standard BHT were added to the vials containing 270 μL of the ABTS mixture solution and kept in dark for 2 h. After incubation, the absorbance of the mixture solution was measured at 734 nm, and the scavenging percentage of ABTS free radical was calculated by following equation 1.…”

Sustainable Utilization of Food Biowaste (Papaya Peel) Extract for Gold Nanoparticle Biosynthesis and Investigation of Its Multi-Functional Potentials

“…The ABTS scavenging effect of VPPE-AuNPs and the standard GA was estimated by a regular process [49]. Before starting the research investigation, 7.4 mM of the ABTS stock solutions and potassium persulfate 2.6 mM were prepared and mixed equally.…”