Multivalent alteration of quorum sensing in Staphylococcus aureus

Yerushalmi, Sarit; Buck, Maren E.; Lynn, David M.; Lemcoff, N. Gabriel; Meijler, Michaël M.

doi:10.1039/c3cc41645c

Cited by 16 publications

(13 citation statements)

References 23 publications

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“…While only few studies have investigated AIP IV as a potential inhibitor of Agr, an interesting study by Yerushalmi et al showed that Agr group IV inhibition can be enhanced in the presence of an AIP IV macromolecule [81]. In that report, the authors conjugated up to 40 AIP IV molecules onto a polymer backbone scaffold using AIP IV monomers modified with a norleucine residue in place of the carboxyl methionine residue.…”

Section: Targeting S Aureus Agr Quorum-sensingmentioning

confidence: 99%

Investigational therapies targeting quorum-sensing for the treatment ofStaphylococcus aureusinfections

Khan

Yeh

Cheung

et al. 2015

Expert Opinion on Investigational Drugs

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Introduction Antibiotic resistance is a serious global health concern for developed and developing nations. Methicillin-resistant Staphylococcus aureus (MRSA) represents a particularly severe public health threat that is associated with high morbidity and mortality. The lack of novel antibiotics has led scientists to explore therapies targeting bacterial virulence mechanisms and virulence regulators, including those controlling cell-cell communication. Areas covered We discuss the role of quorum-sensing in S. aureus infections and components of the system that are being targeted using novel investigational drugs. In particular, the role of the accessory gene regulator (Agr) system in virulence regulation of S. aureus pathogenesis is examined. Finally, we present and compare natural and synthetic compounds that have been found to interfere with Agr functionality. Expert opinion There is a great need to develop new therapeutic methods to combat S. aureus infections. These include anti-virulence therapies that target key global regulators involved with the establishment and propagation of infection. Several molecules have been found to interfere with S. aureus virulence regulation, especially those targeting the Agr quorum-sensing signaling molecule. These preliminary findings warrant further investigation and validation, with the goal of refining a compound that has broad-spectrum inhibitory effects on most S. aureus strains and Agr sub-types.

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Section: Targeting S Aureus Agr Quorum-sensingmentioning

confidence: 99%

Investigational therapies targeting quorum-sensing for the treatment ofStaphylococcus aureusinfections

Khan

Yeh

Cheung

et al. 2015

Expert Opinion on Investigational Drugs

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“…Yet for certain b-lactamases there appears to be a permissive correlation with particular bacteria, implying a fitness cost underlying the control of their expression (Fernández et al 2012). The biochemical basis for the much more complex transition from planktonic bacteria to the biofilm, as regulated by quorum-sensing pathways and correlated to virulence (Beceiro et al 2013), has revealed strategies -chemical (Morkunas et al 2012;Stacy et al 2012Stacy et al , 2013Imperi et al 2013b;Melamed Yerushalmi et al 2013;Saroj andRather 2013), biochemical (Chatterjee et al 2013), and microbiological ) -to interfere with this transition Hirakawa and Tomita 2013;Zhu and Kaufmann 2013). While the determination of the optimal structure and target for intervention is still uncertain (and is likely different for each bacterium), whole-genome analysis of the bacterial resistome (McArthur et al 2013) is a credible strategy to identify lineage in outbreaks Otto 2013b;Reuter et al 2013;Holt et al 2013); to identify resistance-evolution pathways (Abranches et al 2013;Kamen Ek and Gur-Bertok 2013;Méhi et al 2013); to correlate biochemical adaptation to resistance (as exemplified for daptomycin (Kelesidis et al 2013;Peleg et al 2012;Song et al 2013;Tran et al 2013); and to validate targets .…”

Section: Will An Understanding Of How Bacteria Recognize the Presencementioning

confidence: 97%

Strategies for Circumventing Bacterial Resistance Mechanisms

Fisher

Johnson

Mobashery

2014

Handbook of Antimicrobial Resistance

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The future practices for the control of bacterial infections are uncertain. The intransigent infection is no longer found just among the immune compromised but is now found both in and out the boundaries of the hospital. Preserving the efficacy of the antibacterials we have, in order to secure the time needed to discover and develop new antibacterials, will require abrupt change: in the way antibacterials are dispensed and disposed, in the criteria used to measure clinical safety and efficacy, in the financial incentives for antibacterial development, and in the understanding of the molecular mechanisms governing the relationship between the antibacterial and the bacterium. This review examines this relationship from the particular perspective of the eventual need to circumvent resistance mechanisms in order to reclaim the lifesaving value of the antibacterial chemical.

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“…Antibacterial activity of the PDMS-based polymer particles with four concentrations of Ag-rGO (0, 0.5, 1, and 2 mL) was investigated against the bacterium Staphylococcus aureus (ATCC19095). All materials tested had dimensions of 0.5 cm × 1.0 cm and were sterilized with UV light [26]. All bacteria were stored at −80 ∘ C until required.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay

Carreño

Barbosa

Duarte

et al. 2017

Journal of Nanomaterials

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Silver-functionalized reduced graphene oxide (Ag-rGO) nanosheets were prepared by single chemical and thermal processes, with very low concentration of silver. The resulting carbon framework consists of reduced graphene oxide (rGO) sheets or 3D networks, decorated with anchored silver nanoparticles. The Ag-rGO nanosheets were dispersed into a polymer matrix and the composites evaluated for use as biological scaffolds. The rGO material in poly(dimethylsiloxane) (PDMS) has been tested for antimicrobial activity against Gram-positiveStaphylococcus aureus(S. Aureus) bacteria, after exposure times of 24 and 120 hours, as well as in the determination of cell viability on cultures of fibroblast cells (NIH/3T3). Using 1 mL of Ag-rGO in PDMS the antibacterial effectiveness againstStaphylococcus aureuswas limited, showing an increased amount of Colony Forming Units (CFU), after 24 hours of contact. In the cell viability assay, after 48 hours of contact, the group of 1 mL of Ag-rGO with PDMS was the only group that increased cell viability when compared to the control group. In this context, it is believed these behaviors are due to the increase in cell adhesion capacity promoted by the rGO. Thus, the Ag-rGO/PDMS hybrid nanocomposite films can be used as scaffolds for tissue engineering, as they limit antimicrobial activity.

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Multivalent alteration of quorum sensing in Staphylococcus aureus

Cited by 16 publications

References 23 publications

Investigational therapies targeting quorum-sensing for the treatment ofStaphylococcus aureusinfections

Investigational therapies targeting quorum-sensing for the treatment ofStaphylococcus aureusinfections

Strategies for Circumventing Bacterial Resistance Mechanisms

Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay

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