Multivalent, Saccharide‐Functionalized Gold Nanoparticles as Fully Synthetic Analogs of Type A <i>Neisseria meningitidis</i> Antigens

Manea, Flavio; Bindoli, Cristiano; Fallarini, Silvia; Lombardi, Grazia; Polito, Laura; Lay, Luigi; Bonomi, Renato; Mancin, Fabrizio; Scrimin, Paolo

doi:10.1002/adma.200800737

Cited by 52 publications

(31 citation statements)

References 34 publications

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“…Thiolate-protected AuNPs have since become a scaffold for a wide variety of applications, such as molecular electronics, 4 catalysis, 5 biosensing, 6 and vaccine development. 7 …”

Section: Introductionmentioning

confidence: 99%

Characterization of thiolate-protected gold nanoparticles by mass spectrometry

Harkness¹,

Cliffel²,

McLean³

2010

Analyst

115

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Thiolate-protected gold nanoparticles (AuNPs) are a highly versatile nanomaterial, with wide-ranging physical properties dependent upon the protecting thiolate ligands and gold core size. These nanoparticles serve as a scaffold for a diverse and rapidly increasing number of applications, extending from molecular electronics to vaccine development. Key to the development of such applications is the ability to quickly and precisely characterize synthesized AuNPs. While a unique set of challenges have inhibited the potential of mass spectrometry in this area, recent improvements have made mass spectrometry a dominant technique in the characterization of small AuNPs, specifically those with discrete sizes and structures referred to as monolayer-protected gold clusters (MPCs). The unique ability of mass spectrometry to analyze the protecting monolayer of the AuNP may cause it to become a major technique in the characterization of larger AuNPs. The development of mass spectrometry techniques for AuNP characterization has begun to reveal interesting new areas of research. This report is a discussion of the historical challenges in this field, the emerging techniques which aim to meet those challenges, and the future role of mass spectrometry in the growing field of thiolate-protected AuNPs.

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“…Thiolate-protected AuNPs have since become a scaffold for a wide variety of applications, such as molecular electronics, 4 catalysis, 5 biosensing, 6 and vaccine development. 7 …”

Section: Introductionmentioning

confidence: 99%

Characterization of thiolate-protected gold nanoparticles by mass spectrometry

Harkness¹,

Cliffel²,

McLean³

2010

Analyst

115

View full text Add to dashboard Cite

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“…Carbohydrate-functionalized nanomaterials could act as antiadhesion agents avoiding the adhesion of microbes to host cells and thus, becoming an alternative to antibiotic strategy. Manea et al 130 have assembled thiolated synthetic analogues of type A Neisseria meningitides antigens on the surface of gold nanoparticles to inhibit the binding of this bacterium to specific mouse polyclonal antibodies.…”

Section: Gold Gnps As Antiadhesion Agentsmentioning

confidence: 99%

Multivalent glycoconjugates in medicinal chemistry

Fernández-Bolaños¹,

Maya²,

Oliete³

2012

Carbohydrate Chemistry

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“…Peptidefunctionalized Au-NPs have shown the ability to enhance immune responses in cells, 25,26 and gold nanoparticles bearing tumor-associated glycopeptide antigens have recently been reported as promising potential cancer vaccines. 27 We have recently shown 28 that Au-NPs functionalized with oligosaccharides (Glyco-Au-NPs) designed to mimic the polysaccharide capsule that coats serogroup A of the Neisseria meningitidis bacterium 29 (MenA) bind very strongly to polyclonal human antibodies against MenA. At the same nominal concentration of saccharides the nanoparticles showed a more than three orders of magnitude higher binding affinity than their counterparts not bound to the gold cluster: a remarkable result, fully consistent with the multivalent nature of the system.…”

Section: Introductionmentioning

confidence: 98%

Factors affecting T cell responses induced by fully synthetic glyco-gold-nanoparticles

et al. 2013

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We have synthesized and characterized nearly monodisperse and highly pure gold nanoparticles (2 and 5 nm) coated with non-immunoactive mono-and disaccharides, modelled after the capsular polysaccharide of serogroup A of the Neisseria meningitidis bacterium. We have used them to test their ability to induce immune cell responses as a consequence of their multivalency. The results indicate that they are indeed immunoactive and that immunoactivity is strongly dependent on size, and larger, 5 nm nanoparticles perform far better than smaller, 2 nm ones. Immune response (activation of macrophages) initiates with the whole nanoparticle recognition by the surface of antigen-presenting cells, independent of the saccharide oligomerization (or charge) on the nanoparticle surface. The induction of T cell proliferation and the increase of IL-2 levels, a consequence of the expression of MHC II involved in antigen presentation, require the presence of a disaccharide on the nanoparticle, not just a monosaccharide. A possible explanation is that, at this stage, the saccharides are detached from the gold surface. These results may provide leads for designing new saccharide-based, nanoparticle-conjugate vaccines.

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Multivalent, Saccharide‐Functionalized Gold Nanoparticles as Fully Synthetic Analogs of Type A Neisseria meningitidis Antigens

Cited by 52 publications

References 34 publications

Characterization of thiolate-protected gold nanoparticles by mass spectrometry

Characterization of thiolate-protected gold nanoparticles by mass spectrometry

Multivalent glycoconjugates in medicinal chemistry

Factors affecting T cell responses induced by fully synthetic glyco-gold-nanoparticles

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