Multivariate analysis of ‘bacuri’ reproductive and vegetative morphology

Souza, Isis Gomes de Brito; Souza, V. A. B. de; Damasceno‐Silva, Kaesel Jackson; Lima, P. S. da C.

doi:10.14295/cs.v7i2.779

Cited by 6 publications

(7 citation statements)

References 7 publications

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“…Each one is represented mainly by accessions collected in these states or nearby regions. Some efforts to characterize the conserved germplasm have been carried out by Carvalho et al (2002Carvalho et al ( , 2003Carvalho et al ( , 2004 and Souza et al (2016). The germplasm bank accessions of Piauí were molecularly characterized with inter simple sequence repeat (ISSR) markers, identifying genetic differentiation among sampling localities in the states of Maranhão and Piauí (Souza et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of progenies of bacurizeiro ( Platonia insignis ) from Marajó Island, northeastern Amazon

Pontes

Moura

et al. 2017

Acta Amaz.

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The bacurizeiro (Platonia insignis Mart.) is a tree native to the Amazon whose fruit is much used in the gastronomy in the North and Northeast regions of Brazil. Due to its great economic potential for these regions, the species is being conserved in germplasm banks to support genetic breeding programs. The aim of this work was the molecular characterization of P. insignis accessions belonging to the germplasm bank of the Embrapa Eastern Amazon research unit using ISSR (Inter Simple Sequence Repeat) markers. Seventy-eight accessions of P. insignis belonging to 16 progenies were sampled in two different localities on Marajó Island, state of Pará, Brazil. Among the 16 progenies, seven were collected in Soure and nine in Salvaterra. The 78 accessions were genotyped with 23 pre-selected primers. We obtained 121 amplified products, of which 54 were polymorphic. The most polymorphic primers were UBC 834, UBC 899 and UBC 900. Primers UBC810 and UBC884 did not amplify polymorphic bands. Forty-nine markers out of 54 were selected for genetic analyses. AMOVA within and among progenies showed low genetic differentiation (Φ PT = 0.064, P<0.001) with higher diversity within progenies (96%), low genetic differentiation among sampling localities (Φ PT = 0.025, P<0.013), and higher diversity within (98%) than among localities. Clustering by UPGMA based on Jaccard similarities among pairs of accessions did not separate genotypes according to progeny or sampling localitiy. We recommend that new germplasm surveys consider a greater sampling effort within sampling localitites. KEYWORDS: genetic diversity, germplasm bank, Clusiaceae Caracterização molecular de progênies de bacurizeiro (Platonia insignis) da Ilha de Marajó, nordeste da Amazônia RESUMO O bacurizeiro (Platonia insignis Mart.) é uma espécie frutífera nativa da Amazônia muito utilizada na cultura alimentar nas regiões Norte e Nordeste do Brasil. Devido a seu grande potencial econômico regional, a espécie vem sendo conservada em bancos ativos de germoplasma (BAG) para apoiar programas de melhoramento genético. Dessa forma, o objetivo deste trabalho foi caracterizar molecularmente acessos de P. insignis pertencentes ao BAG da Embrapa Amazônia Oriental por meio de marcadores ISSR (Inter Simple Sequence Repeat). Foram coletados 78 acessos de P. insignis pertencentes a 16 progênies coletadas em dois locais diferentes na Ilha de Marajó, PA. Das 16 progênies, sete foram coletadas em Soure e nove em Salvaterra. Os 78 acessos foram genotipados com 23 primers ISSR pré-selecionados. Obteve-se 121 produtos amplificados, dos quais 54 foram polimórficos. Os primers mais polimórficos foram UBC 834, UBC 899 e UBC 900. Já os primers UBC810 e UBC884 não apresentaram bandas polimórficas. Das 54 marcas, 49 foram selecionadas para as análises genéticas. A AMOVA entre e dentro de progênies identificou baixa diferenciação genética (Φ PT = 0,064, P<0,001) com maior diversidade dentro de progênies (96%), bem como baixa diferenciação genética entre os locais de coleta (Φ PT = 0,025, P<0,0...

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of progenies of bacurizeiro ( Platonia insignis ) from Marajó Island, northeastern Amazon

Pontes

Moura

et al. 2017

Acta Amaz.

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“…Caatinga vegetation includes several endemic species, such as Spondias tuberosa Arruda (Anacardiaceae), popularly known as umbu tree (Figs. 1a and 1b) (Souza 2000; Lins Neto et al 2010Neto et al , 2013Mitchell and Daly 2015). Its popular name is derived from the Tupi-Guarani indigenous word "ymb-u", which means "the tree that gives water" (Epstein 1998).…”

Section: Introductionmentioning

confidence: 99%

“…tuberosa is an andromonoecious species with gametophytic self-incompatibility (Leite and Machado 2010), pollinated mainly by bees and wasps (Nadia et al 2007; Almeida et al 2011). It is a predominantly allogamous species, with an estimated outcrossing rate of 74% (Souza 2000), and variation between 80.4% (multi-locus) and 84.1% (single locus) (Santos and Gama 2013). However, fruit production is low, considering the high number of owers produced (Fig.…”

Section: Introductionmentioning

confidence: 99%

SNP-based analysis reveals high genetic structure and diversity in umbu tree (Spondias tuberosa Arruda), a native and endemic species of the Caatinga biome

do Nascimento,

Costa,

Alves-Pereira

et al. 2024

Genet Resour Crop Evol

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Umbu (Spondias tuberosa Arruda) is an endemic fruit tree restricted to the Brazilian seasonally dry tropical forest called Caatinga. This study aimed to evaluate the structure and genomic diversity of umbu trees from seven locations in the Caatinga biome, distributed among four Brazilian states. Using genotyping-by-sequencing (GBS), a total of 5,336 SNPs were obtained, of which 250 showed outlier behavior. Therefore, 5,086 neutral SNPs were used for population structure and genetic diversity analyses. Both discriminant analysis of principal components (DAPC) and neighbor-joining cluster analyses classi ed the accessions into four groups, with a genetic structure observed among groups, disagreeing with our initial hypothesis of low genetic structure between locations. Isolation by distance (r 2 = 0.974; p = 0.0015) was detected. Moderate to high levels of genetic diversity were found, with the average observed heterozygosity (H O = 0.221) higher than the expected heterozygosity (H E = 0.199) and with negative inbreeding coe cient (F IS ) values. Most genetic variation was found within locations, although high diversity between locations (22.1%) was observed. The results obtained are important for understanding the levels and distribution of genetic variation, suggesting that most locations are priorities for conservation actions, contributing with different alleles to the species' gene pool in Brazil.

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“…Exploratory multivariate statistical techniques are usually applied in studies with genotype assessment, such as in cowpea (Santos et al, 2016) or in fruit-bearing species, as is the case of bacurizeiro (Souza et al, 2016). However, this technique has potential for seed quality assessment, a fact proven for soybean (Barbosa et al, 2013) and Brachiaria brizantha cv.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of forage pea seed lots by means of multivariate techniques

Machado¹,

Martins²,

Silva³

et al. 2019

Científica

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Multivariate techniques allow to understand the structural dependence contained in the variables, as well as to characterize groups of seed lots according to specific standards. Thus, this study analyzes the efficiency of multivariate exploratory techniques in discriminating forage pea seed lots as a function of the physiological potential of seeds. We evaluated ten seed lots of forage pea in a completely randomized design, considering the following variables: thousand seed weight, germination, first germination count, electrical conductivity, and accelerated aging. Moreover, seedling emergence, first count of seedlings in the field, and seedling emergence speed index in the field were added to randomized blocks with four replications per lot. Initially, the data obtained in each test were analyzed separately by means of analysis of variance, and the means of the treatments were compared by the Scott Knott test at 5% probability. Exploratory multivariate statistical techniques were applied by means of Cluster Analysis and Principal Components Analysis to discriminate seed lots with better physiological quality and to characterize the variables responsible for the differentiation between them. Multivariate analysis of principal components is efficient in discriminating vigor and seed germination tests in Pisum sativum subsp. Arvense, which help in identifying lots of superior performance in the field.

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Multivariate analysis of ‘bacuri’ reproductive and vegetative morphology

Cited by 6 publications

References 7 publications

Molecular characterization of progenies of bacurizeiro ( Platonia insignis ) from Marajó Island, northeastern Amazon

Molecular characterization of progenies of bacurizeiro ( Platonia insignis ) from Marajó Island, northeastern Amazon

SNP-based analysis reveals high genetic structure and diversity in umbu tree (Spondias tuberosa Arruda), a native and endemic species of the Caatinga biome

Discrimination of forage pea seed lots by means of multivariate techniques

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