Multivariate and integrative approach to analyze multiple biomarkers in ecotoxicology: A field study in Neotropical region

Ghisi, Nédia de Castilhos; Oliveira, Elton Celton de; Guiloski, Izonete Cristina; Lima, Sonia Barbosa de; Assis, Helena C. Silva de; Longhi, Solon Jonas; Prioli, Alberto José

doi:10.1016/j.scitotenv.2017.07.266

Cited by 27 publications

(7 citation statements)

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“…Geophagus brasiliensis , in a Brazilian River, had an increase of DNA damage associated with Fe, Mn, Cd, Cu, and Pb ( Gomes et al., 2019 ). Ghisi et al. (2017) found a higher frequency of DNA damage in Astyanax aff.…”

Section: Resultsmentioning

confidence: 93%

“…Geophagus brasiliensis, in a Brazilian River, had an increase of DNA damage associated with Fe, Mn, Cd, Cu, and Pb (Gomes et al, 2019). Ghisi et al (2017) found a higher frequency of DNA damage in Astyanax aff. paranae located in a site downstream of the urban area, which received a mix of pollutants, including industrial and municipal wastewater.…”

Section: Genotoxicitymentioning

confidence: 89%

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Genotoxic effect of heavy metals on Astyanax lacustris in an urban stream

Silva

Benvindo‐Souza

Cotrim

et al. 2020

Heliyon

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Uncontrolled urbanization growth contributes to the pollution of aquatic environments. Heavy metals released by domestic and industrial effluents can negatively affect aquatic organisms. This study aimed to evaluate the effect of environmental pollutants, such as metals, on fish DNA damage, in stretches of an urban stream. Specimens of the Neotropical fish, Astyanax lacustris , were exposed in situ for 96 h along the Antas stream, a Brazilian aquatic system deteriorated by anthropogenic factors. Water and sediment samples were collected simultaneously for physicochemical and heavy metal analyses. The comet assay was performed as a biomarker of genotoxicity. Fish located downstream had a higher frequency of DNA damage than in the reference site. We found concentrations of Cr and Ni above acceptable levels in sediment samples. Generally, Ba, Mn, Mg, Zn, Cr, and Ni were the elements most associated with genotoxic damage. Water and sediment of the Antas stream showed genotoxic potential in A. lacustris according to the urbanization gradient, demonstrating the importance to prevent the release of environmental pollutants, especially heavy metals in urban areas.

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Section: Resultsmentioning

confidence: 93%

Section: Genotoxicitymentioning

confidence: 89%

Genotoxic effect of heavy metals on Astyanax lacustris in an urban stream

Silva

Benvindo‐Souza

Cotrim

et al. 2020

Heliyon

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“…Fish exposed to chlorpyrifos showed a significant increase in CAT activity in gills (Figure 3D), which generally indicates activation of the antioxidant defense system, preventing oxidative stress (Clemente et al 2010; Ghisi et al 2017). This recorded induction may be sufficient to cope with oxidative stress induced by the toxic substance because no significant increase was found in the levels of LPO, as has been reported in another study (Almeida and Oliveira 2010).…”

Section: Resultsmentioning

confidence: 93%

Early Toxic Effects in a Central American Native Fish (Parachromis dovii) Exposed to Chlorpyrifos and Difenoconazole

Jiménez

Solano

Scholz

et al. 2021

Enviro Toxic and Chemistry

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In Costa Rica, agriculture is one of the most important economic activities. Chlorpyrifos and difenoconazole have been identified as agrochemicals widely used in banana and pineapple crops in the Caribbean area of the country and are constantly recorded in aquatic ecosystems. The toxicity of these pesticides in Parachromis dovii was studied. Median lethal concentrations (LC50s) for each substance were obtained from 96-h acute tests. Then, fish were exposed to sublethal concentrations of both substances (10% of LC50), individually and in mixture, to evaluate biomarker responses. Ethoxyresorufin-O-deethylase (EROD), catalase, and glutathione S-transferase activities as well as lipid peroxidation were measured in liver and gill tissues as markers of biotransformation and oxidative stress processes. Cholinesterase activity in brain and muscle tissue was also quantified as a biomarker of toxicity. The LC50s were 55.34 μg/L (95% confidence interval [CI] 51.06-59.98) for chlorpyrifos and 3250 μg/L (95% CI 2770-3810) for difenoconazole. Regarding the biomarkers, a significant inhibition of brain and muscle cholinesterase activity was recorded in fish exposed to 5.50 μg/L of chlorpyrifos. This activity was not affected when fish were exposed to the mixture of chlorpyrifos with difenoconazole. Significant changes in lactate dehydrogenase activity were observed in fish exposed to 325 μg/L of difenoconazole, whereas fish exposed to the mixture showed a significant increase in EROD activity in the liver. These results suggest harmful effects of chlorpyrifos insecticide at environmentally relevant concentrations. There is also evidence for an interaction of the 2 substances that affects the biotransformation metabolism at sublethal levels of exposure.

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“…Passive monitoring refers to organisms sampled in local populations whereas active implies the exposure of organisms in sites to be monitored for a period of time. Nevertheless, most of the biomonitoring surveys developed until now have been focused on the passive monitoring methodology, with the use of vertebrates and invertebrates (Ghisi et al, 2017;Ronci et al, 2016;Ruchter and Sures, 2015), as well as some aquatic plants species (Bonanno et al, 2017;Demirezen and Aksoy, 2004;Gonzalez et al, 2013;Harguinteguy et al, 2016;P erez et al, 2017) as bioindicators. Passive biomonitoring has been widely used for its sampling simplicity, long-term measurements and existing guidelines.…”

Section: Introductionmentioning

confidence: 99%