Multivariate evaluation of inhibition studies on an enzyme electrodes system with pesticides and mixtures of mercury and pesticides

Danzer, Thomas; Schwedt, Georg

doi:10.1016/0003-2670(95)00598-6

Cited by 25 publications

(9 citation statements)

References 10 publications

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“…The different approaches have been reported that employ various enzymes for the detection of OP compounds and carbamates in mixtures in combination with chemometric data processing (Danzer and Schwedt, 1996). In other study, engineered variants of Drosophila melanogaster AChE were used as biological receptors in AChE -multisensors for simultaneous detection and discrimination of binary mixtures of cholinesterase-inhibiting compounds (Bachmann et al, 2000).…”

Section: Interference Studymentioning

confidence: 99%

Immobilization of acetylcholineesterase–choline oxidase on a gold–platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents

Upadhyay

Rao

Sharma

et al. 2009

Biosensors and Bioelectronics

159

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Section: Interference Studymentioning

confidence: 99%

Immobilization of acetylcholineesterase–choline oxidase on a gold–platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents

Upadhyay

Rao

Sharma

et al. 2009

Biosensors and Bioelectronics

159

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“…Since the originality and structure of cholinesterase itself can define its affinity for different OP compounds, disposable multielectrode biosensors have been developed for discrimination of binary mixtures of OPs and carbamates by combining native and recombinant variants of AChE with chemometric data processing. 25,27,28 The assay duration was 40 min and the limit of detection (LOD) for paraoxon and carbofuran in drinking water in separate analyses was 0.5 mg mL À1 . For inhibition analysis of binary mixtures, the sensor displayed a resolution error of 0.4 mg mL À1 for paraoxon and 0.5 mg mL À1 for carbofuran, respectively.…”

Section: Introductionmentioning

confidence: 99%

Detection of mixed organophosphorus pesticides in real samples using quantum dots/bi-enzyme assembly multilayers

Zheng

Dai

et al. 2011

J. Mater. Chem.

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“…These developments fall into two branches: enzyme biosensors and bioreactors. The carbamate and phosphoric acid ester insecticide can be detected by a pH electrode modified with an acetylcholinesterase (AChE) layer 3–5. A biosensor based on the quartz crystal microbalance with immobilized AChE has been developed for the determination of organophosphorous and carbamate pesticides 6…”

Section: Introductionmentioning

confidence: 99%

Flow analysis for determination of paraoxon with use of immobilized acetylcholinesterase reactor and new type of chemiluminescent reaction

Danet

Badea²,

Marty

et al. 2000

Biopolymers

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A highly sensitive flow analysis method for determination of acetylcholinesterase (AChE) inhibitors like organophosphorous pesticides using a new chemiluminescent reaction was developed and optimized. This method is fast, sensitive, and cheap, because it requires only one enzyme and its substrate. The system incorporates a reactor with immobilized AChE on controlled pore glass (CPG) and a chemiluminometric detector. Variations in enzyme activity due to inhibition are measured from the changes of concentrations of thiocholine produced when the substrate (acetylthiocholine chloride) is pumped before and after the passage of the solution containing the pesticide through the immobilized AChE reactor. Thiocholine is determined by a new chemiluminescent reaction with luminol in the presence of potassium ferricyanide. The percentage inhibition of enzyme activity is correlated to the pesticide concentration. The inhibited enzyme is reactivated by 10 mM pyridine‐2‐aldoxime methiodide (2‐PAM). The experimental conditions were first optimized for activity determination of the effect of pH, flow rates, and Tris concentrations. For the measurement of AChE inhibition, the appropriate concentration of the substrate is selected such that the rate of noninhibited reaction can be considered unchanged and could be used as a reference. For optimization of experimental conditions for inhibition, several parameters of the system are studied and discussed: flow rate, enzyme–pesticide contact time, luminol concentration, ferricyanide concentration, 2‐PAM concentration, and configuration of the FIA manifold. Paraoxon, an organophosphorous pesticide was tested. For an inhibition time of 10 min the calibration graph is linear from 0.1 to 1 ppm paraoxon with a relative standard deviation (n = 5) of 4.6% at 0.5 ppm. For an inhibition time of 30 min the calibration graph is linear from 25 to 250 ppb paraoxon. © 2000 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 57: 37–42, 2000

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Multivariate evaluation of inhibition studies on an enzyme electrodes system with pesticides and mixtures of mercury and pesticides

Cited by 25 publications

References 10 publications

Immobilization of acetylcholineesterase–choline oxidase on a gold–platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents

Immobilization of acetylcholineesterase–choline oxidase on a gold–platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents

Detection of mixed organophosphorus pesticides in real samples using quantum dots/bi-enzyme assembly multilayers

Flow analysis for determination of paraoxon with use of immobilized acetylcholinesterase reactor and new type of chemiluminescent reaction

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