Multivariate extension of the continuous variation and mole-ratio methods for the study of the interaction of intercalators with polynucleotides

Spectrometric study of the interaction between Alpinetin and bovine serum albumin using chemometrics approaches

“…This supports the EFA results above that there were three chemical components in the system-namely, the free BSA, free APT and the BSA-APT complex. Consequently, the apparent binding constant K app [27] may be calculated from the extracted spectral profiles:…”

Effects of aluminum on amyloid-beta aggregation in the context of Alzheimer’s disease

“…The MCR-ALS procedure calculates the C and S matrices in turn by the least squares method, and the iterative process is repeated until convergence. When the number of species, F, and the initial estimates, which are required to initiate the iterative ALS procedure (Vives et al, 2000), have been extracted, the concentration profiles and the pure spectra of all the contributing species can be established by the MCR-ALS method.…”

Variable path length and counter-flow continuous variation methods for the study of the formation of high-affinity complexes by absorbance spectroscopy. An application to the studies of substrate binding in cytochrome P450

“…Although the formalism above is strictly valid only in the case of "all-or-none" binding to n identical binding sites, its applicability may be extended to more complex cases by studying the dependency of the shape of Job's plot on C 0 [4]. In addition to offering a simple way to determine the stoichiometry of complex formation, continuous variation titration provides an excellent experimental strategy for determination of the parameters of tight ligand binding, which is the most appropriate when the values of the dissociation constant(s) are comparable in magnitude with the minimal enzyme concentration required for positive monitoring of the interactions [4,8]. The most common implementation of Job's method in enzymology is based on activity measurements, whereas use of this method with absorbance spectroscopy is quite rare due to decreased sensitivity at low enzyme concentrations.…”