Multiview optical resolution photoacoustic microscopy

Zhu, Linyu; Li, Lei; Gao, Liang; Wang, Lihong V.

doi:10.1364/optica.1.000217

Cited by 42 publications

(37 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 In addition, a multiview OR-PAM, which images the sample from multiple view angles and then reconstructs the image with a multiview deconvolution method, has been proposed to realize three-dimensional (3-D) optical resolution. 12 However, it was demonstrated only in (semi-)transparent samples, such as zebra fish.…”

Section: Introductionmentioning

confidence: 99%

Grueneisen relaxation photoacoustic microscopyin vivo

Shi

Hai

et al. 2016

J. Biomed. Opt

Self Cite

View full text Add to dashboard Cite

Abstract. Grueneisen relaxation photoacoustic microscopy (GR-PAM) can achieve optically defined axial resolution, but it has been limited to ex vivo demonstrations so far. Here, we present the first in vivo image of a mouse brain acquired with GR-PAM. To induce the GR effect, an intensity-modulated continuous-wave laser was employed to heat absorbing objects. In phantom experiments, an axial resolution of 12.5 μm was achieved, which is sixfold better than the value achieved by conventional optical-resolution PAM. This axial-resolution improvement was further demonstrated by imaging a mouse brain in vivo, where significantly narrower axial profiles of blood vessels were observed. The in vivo demonstration of GR-PAM shows the potential of this modality for label-free and high-resolution anatomical and functional imaging of biological tissues.

show abstract

Section: Introductionmentioning

confidence: 99%

Grueneisen relaxation photoacoustic microscopyin vivo

Shi

Hai

et al. 2016

J. Biomed. Opt

Self Cite

View full text Add to dashboard Cite

show abstract

“…In focused-transducer-based PAM, Hilbert transformation is usually taken along the A-line direction (the acoustic receiving direction), and then the absolute value of the transformed A-line is taken to produce its envelope. 26,28,29 In linear-array-based PACT, a common choice for the Hilbert transformation direction is the array receiving direction (the depth direction) in the reconstructed images, taking the absolute value extracts the envelope information of images. 12,30 However, in FR-PACT, the acoustic signals are received from all directions, up to an angle of 2π in plane, which makes the determination of the transformation direction difficult.…”

Section: Introductionmentioning

confidence: 99%

Multiview Hilbert transformation in full-ring transducer array-based photoacoustic computed tomography

Zhu

Shen

et al. 2017

J. Biomed. Opt

Self Cite

View full text Add to dashboard Cite

transformation in full-ring transducer array-based photoacoustic computed tomography," J. Biomed. Opt. 22(7), 076017 (2017), doi: 10.1117/1.JBO.22.7.076017. Abstract. Based on the photoacoustic (PA) effect, PA tomography directly measures specific optical absorption, i.e., absorbed optical energy per unit volume. We recently developed a full-ring ultrasonic transducer arraybased photoacoustic computed tomography (PACT) system for small-animal whole-body imaging. The system has a full-view detection angle and high in-plane resolution (∼100 μm). However, due to the bandpass frequency response of the piezoelectric transducer elements and the limited elevational detection coverage of the full-ring transducer array, the reconstructed images present bipolar (i.e., both positive and negative) pixel values, which cause ambiguities in image interpretation for physicians and biologists. We propose a multiview Hilbert transformation method to recover the unipolar initial pressure for full-ring PACT. The effectiveness of the proposed algorithm was first validated by numerical simulations and then demonstrated with ex vivo mouse brain structural imaging and in vivo mouse whole-body imaging.

show abstract

“…The lateral resolution of OR-PAM depends on the diffraction limited spot size produced by the lens with a maximum depth imaging of 1.2 mm. 52,53 OR-PAM was used to image small animal models and biological samples with various endogenous or exogenous contrasts. 52 AR-PAM is a promising imaging technique for both preclinical and clinical applications as deep imaging of biological tissues possible with lower frequency transducers.…”

Section: Introductionmentioning

confidence: 99%

“…52,53 OR-PAM was used to image small animal models and biological samples with various endogenous or exogenous contrasts. 52 AR-PAM is a promising imaging technique for both preclinical and clinical applications as deep imaging of biological tissues possible with lower frequency transducers. 53 Conventional PA imaging systems require expensive ultrafast lasers with nanosecond pulse widths, an ultrasound transducer, and an aqueous coupling medium for the PA waves to propagate through.…”

Section: Introductionmentioning

confidence: 99%

Low-power noncontact photoacoustic microscope for bioimaging applications

2017

View full text Add to dashboard Cite

Abstract. An inexpensive noncontact photoacoustic (PA) imaging system using a low-power continuous wave laser and a kilohertz-range microphone has been developed. The system operates in both optical and PA imaging modes and is designed to be compatible with conventional optical microscopes. Aqueous coupling fluids are not required for the detection of the PA signals; air is used as the coupling medium. The main component of the PA system is a custom designed PA imaging sensor that consists of an air-filled sample chamber and a resonator chamber that isolates a standard kilohertz frequency microphone from the input laser. A sample to be examined is placed on the glass substrate inside the chamber. A laser focused to a small spot by a 40× objective onto the substrate enables generation of PA signals from the sample. Raster scanning the laser over the sample with micrometer-sized steps enables high-resolution PA images to be generated. A lateral resolution of 1.37 μm was achieved in this proof of concept study, which can be further improved using a higher numerical aperture objective. The application of the system was investigated on a red blood cell, with a noiseequivalent detection sensitivity of 43,887 hemoglobin molecules (72.88 × 10 −21 mol or 72.88 zeptomol). The minimum pressure detectable limit of the system was 19.1 μPa. This inexpensive, compact noncontact PA sensor is easily integrated with existing commercial optical microscopes, enabling optical and PA imaging of the same sample. Applications include forensic measurements, blood coagulation tests, and monitoring the penetration of drugs into human membrane.

show abstract

Multiview optical resolution photoacoustic microscopy

Cited by 42 publications

References 21 publications

Grueneisen relaxation photoacoustic microscopyin vivo

Grueneisen relaxation photoacoustic microscopyin vivo

Multiview Hilbert transformation in full-ring transducer array-based photoacoustic computed tomography

Low-power noncontact photoacoustic microscope for bioimaging applications

Contact Info

Product

Resources

About