2016
DOI: 10.3389/fmicb.2016.00534
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Municipal Solid Waste Landfills Harbor Distinct Microbiomes

Abstract: Landfills are the final repository for most of the discarded material from human society and its “built environments.” Microorganisms subsequently degrade this discarded material in the landfill, releasing gases (largely CH4 and CO2) and a complex mixture of soluble chemical compounds in leachate. Characterization of “landfill microbiomes” and their comparison across several landfills should allow the identification of environmental or operational properties that influence the composition of these microbiomes … Show more

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Cited by 106 publications
(93 citation statements)
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“…Reactions were purified using AmpureXP paramagnetic beads (Beckman Coulter Inc., Indianapolis, IN) at a final concentration of 0.8 x v/v. After purification, 4 μL of PCR product was used in a barcoding reaction, cleaned, concentrated, and pooled in equimolar amounts as previously described (31). The pooled, prepared library was then submitted for sequencing on the Illumina MiSeq platform (Illumina Inc., San Diego, CA) using V2 PE250 chemistry.…”
Section: Methodsmentioning
confidence: 99%
“…Reactions were purified using AmpureXP paramagnetic beads (Beckman Coulter Inc., Indianapolis, IN) at a final concentration of 0.8 x v/v. After purification, 4 μL of PCR product was used in a barcoding reaction, cleaned, concentrated, and pooled in equimolar amounts as previously described (31). The pooled, prepared library was then submitted for sequencing on the Illumina MiSeq platform (Illumina Inc., San Diego, CA) using V2 PE250 chemistry.…”
Section: Methodsmentioning
confidence: 99%
“…Initial sample 16S/18S rRNA PCR amplification was performed in duplicate 25 μL reactions as follows: 1x QuantaBio 5PRIME HotMasterMix, 0.2 μM 515-Y Forward Primer (5’- GTA AAA CGA CGG CCA GT CCG TGY CAG CMG CCG CGG TAA-3’) where the M13 forward primer (underlined) is ligated to the small sub-unit RNA (ssuRNA) specific sequence via a ‘CC’ spacer, 0.2 μM 926R (5’-CCG YCA ATT YMT TTR AGT TT-3’) Reverse Primer and template DNA. Reverse primers were as reported by Parada et al (2015) (32) while forward primers were adapted from Parada et al with the addition of the M13 sequence, as done by Stamps et al (2016) (33). Primers from Parada et al target all three domains of life (32).…”
Section: Methodsmentioning
confidence: 99%
“…A second, 6 cycle, PCR amplification step with the parameters used in the first amplification step was used to ligate barcodes adapted from Caparaso et. al (2012) (34) to the M13 region of the forward primer (33). PCR product cleanup was performed with 0.8x concentration of KAPA Pure Beads (Kapa Biosystems, Indianapolis, IN) according to the manufacturer’s specifications.…”
Section: Methodsmentioning
confidence: 99%
“…) and landfills (Stamps et al . ). Intensive research in relatively “old” landfills is needed to assess the chemical and physical compositions of these sites, as well as for microbial sampling and analysis.…”
Section: Developing Markers Of the Anthropocene From Landfill Microbimentioning
confidence: 97%
“…For instance, recent work focusing on the microbiomes of leachates from 19 non‐hazardous landfills highlighted the potential that microbiomes have as anthropogenic markers (Stamps et al . ). Profiling may initially focus on 16S rRNA gene sequencing along with metagenomic and metatranscriptomic data before advanced mechanistic questions are addressed.…”
Section: Developing Markers Of the Anthropocene From Landfill Microbimentioning
confidence: 97%