Mupirocin W (I), a Novel Pseudomonic Acid Produced by Targeted Mutation of the Mupirocin Biosynthetic Gene Cluster.

Cooper, Sian M.; Cox, Russell J.; Crosby, John; Crump, Matthew P.; Hothersall, Joanne; Laosripaiboon, Wanpen; Simpson, Thomas J.

doi:10.1002/chin.200530197

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Hplc Analysis Of the Products Of The Wt And Mutant Strains1

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2005

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“…Maximum an-common to all known pseudomonic acids. Detailed structural analysis is described fully elsewhere [19]. tibiotic production (w0.8 g/ml) was reached at approximately 15 hr, and from this point, levels of PA-A remained relatively constant.…”

Section: Hplc Analysis Of the Products Of The Wt And Mutant Strainsmentioning

confidence: 99%

Shift to Pseudomonic Acid B Production in P. fluorescens NCIMB10586 by Mutation of Mupirocin Tailoring Genes mupO, mupU, mupV, and macpE

Cooper

Laosripaiboon

Rahman

et al. 2005

Chemistry & Biology

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Mupirocin, a polyketide-derived antibiotic from Pseudomonas fluorescens NCIMB10586, is a mixture of pseudomonic acids (PA) that target isoleucyl-tRNA synthase. The mup gene cluster encodes both type I polyketide synthases and monofunctional enzymes that should play a role during the conversion of the product of the polyketide synthase into the active antibiotic (tailoring). By in-frame deletion analysis of selected tailoring open-reading frames we show that mupQ, mupS, mupT, and mupW are essential for mupirocin production, whereas mupO, mupU, mupV, and macpE are essential for production of PA-A but not PA-B. Therefore, PA-B is not simply produced by hydroxylation of PA-A but is either a precursor of PA-A or a shunt product. In the mupW mutant, a new metabolite lacking the tetrahydropyran ring is produced, implicating mupW in oxidation of the 16-methyl group.

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Section: Hplc Analysis Of the Products Of The Wt And Mutant Strainsmentioning

confidence: 99%

Shift to Pseudomonic Acid B Production in P. fluorescens NCIMB10586 by Mutation of Mupirocin Tailoring Genes mupO, mupU, mupV, and macpE

Cooper

Laosripaiboon

Rahman

et al. 2005

Chemistry & Biology

Self Cite

View full text Add to dashboard Cite

show abstract

Mupirocin W (I), a Novel Pseudomonic Acid Produced by Targeted Mutation of the Mupirocin Biosynthetic Gene Cluster.

Cited by 1 publication

References 1 publication

Shift to Pseudomonic Acid B Production in P. fluorescens NCIMB10586 by Mutation of Mupirocin Tailoring Genes mupO, mupU, mupV, and macpE

Shift to Pseudomonic Acid B Production in P. fluorescens NCIMB10586 by Mutation of Mupirocin Tailoring Genes mupO, mupU, mupV, and macpE

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