Muricauda alvinocaridis sp. nov., isolated from shrimp gill from the Okinawa Trough

Abstract

A Gram-stain-negative, strictly aerobic, non-motile, long, straight-rod and non-flagellated marine bacterium strain, designated SCR12T, was isolated from the gill of a shrimp collected in the Tangyin hydrothermal field of the Okinawa Trough. The growth temperature was in the range of 16–40 °C and the optimum temperature was 37 °C. Optimal growth occurred at pH 6.5 and in the presence of 3 % (w/v) NaCl. The predominant isoprenoid quinone of strain SCR12T was menaquinone-6 (MK-6). The predominant fatty acids (>10 %) were iso-C15 : 0 (44.2 %), iso-C15 : 1 G (19.0 %) and iso-C17 : 0 3-OH (12.1 %). The major polar lipids comprised one phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminolipids and three unidentified lipids. Based on the results of 16S rRNA gene sequence analysis, strain SCR12T was found to be most closely related to Muricauda olearia CL-SS4T (98.09 %), followed by Muricauda beolgyonensis BB-My12T (97.65 %), Muricauda aquimarina SW-63T (97.58 %) and Muricauda ruestringensis DSM 13258T (97.31 %) and with lower sequence similarities (95.74–97.10 %) to other species of the genus Muricauda . Genome relatedness between strain SCR12T and M. olearia CL-SS4T was computed using both average nucleotide identity (ANI) and DNA–DNA hybridization (DDH) and resulted in values of 85.6 % and 29.3±2.3 %, respectively. The genomic DNA G+C content of strain SCR12T was 42.3 mol%. On the basis of polyphasic analysis, the strain SCR12T was considered to represent a novel species of the genus Muricauda, for which the name Muricauda alvinocaridis sp. nov. is proposed. The type strain is SCR12T (=MCCC 1K03731T=JCM 33425T).

Muricauda brasiliensis sp. nov., isolated from a mat-forming cyanobacterial culture

“…The draft genome of strain K001 is 3.79 Mb, distributed in 55 contigs [26]. The GC content is 41.62 mol%, matching with the expected range to the genus Muricauda (39.9-50.70 mol%) [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. The average coverage was approximately 300x, and the N50 and L50 values for the assembly were 232,816 bp and 5 contigs, respectively [26].…”

“…The name of the group refers to an appendage contiguous to the outer membrane present in some cells of species belonging to the genus [1]. As of January 2020, the genus is comprised of 19 [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. All members of the genus are rod-shaped Gram-negative bacteria isolated from a variety of saline environments such as intertidal, deepsea, and mangrove sediments; surface seawater; sand; and oilpolluted seawater [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].…”

Muricauda maritima sp. nov., Muricauda aequoris sp. nov. and Muricauda oceanensis sp. nov., three marine bacteria isolated from seawater

“…At the time of writing (August 2020), the genus Muricauda consisted of 25 species with validly published names (www.bacterio.net/genus/muricauda) [4]. The genus has been isolated from marine and marine-derived habitats, including seawater [5–7], salt lake [2], coastal hot spring [8], tidal flat [9–11], sediment [1, 12, 13], shrimp gill [14] and sponge [15]. Members of the genus Muricauda are Gram-stain-negative, facultatively anaerobic or strictly aerobic [16].…”

Muricauda onchidii sp. nov., isolated from a marine invertebrate from South China Sea, and transfers of Flagellimonas algicola, Flagellimonas pacifica and Flagellimonas maritima to Muricauda algicola comb. nov., Muricauda parva nom. nov. and Muricauda aurantiaca nom. nov., respectively, and emended description of the genus Muricauda

“…193 [5]. At the time of writing, Muricauda comprises 28 species, which were all isolated from marine environments, such as Muricauda lutaonensis from a coastal hot spring [6], four species from marine tidal flats [7][8][9][10], Muricauda aquimarina and Muricauda flavescens from a salt lake [2], Muricauda amoyensis from coast sand [11], eight species from sea water [3,[12][13][14][15][16], Muricauda koreensis from seaweed [17], five species from sea sediments [1,[18][19][20][21], Muricauda eckloniae from marine alga [22], Muricauda flava and Muricauda hymeniacidonis from marine sponges [23,24], Muricauda marina from marine snow [25], Muricauda amphidinii from dinoflagellate [26], and Muricauda alvinocaridis from shrimp gill [27]. M. eckloniae, M. flava and M. koreensis were described as Flagellimonas eckloniae, Spongiibacterium flavum and Flagellimonas aquimarina, respectively, but García merged Flagellimonas and Spongiibacterium into Muricauda and these three species were transferred to Muricauda.…”