Murine FGF-4 gene expression is spatially restricted within embryonic skeletal muscle and other tissues

Drucker, Beverly; Goldfarb, Mitchell

doi:10.1016/0925-4773(93)90073-7

Cited by 50 publications

(32 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings accentuate the concept that dur-mediated through a KGFR signalling pathway. While ing organogenesis, KGF expressing mesenchyme is in FGF-4 transcripts are expressed in several regions of intimate contact with epithelium expressing KGFR head and limb muscle precursors (Niswander and MarmRNA. In addition, we have demonstrated KGF tin, 1992;Drucker and Goldfarb, 1993), expression is mRNA expression in 6-8 week whole human embryo not detectable after day 13.5, suggesting that it is not tissue. While this corresponds to an earlier stage of involved in the terminal phases of muscle differentiadevelopment than the mouse embryos used for in situ tion.…”

Section: Discussionmentioning

confidence: 76%

“…In particular, various members of the fibroblast growth factor (FGF) gene family have been implicated in mesoderm induction in amphibians, and appear to play a role in early pattern formation (Kimelman and Kirschner, 1987;Slack et al, 1987;Paterno et al, 1989). Furthermore, expression of these genes in later stages of mouse embryonic development implies a role in tissue differentiation and organogenesis (Wilkinson et al, 1989;Hebert et al, 1990;Haub and Goldfarb, 1991;Niswander and Martin, 1992;Drucker and Goldfarb, 1993;deLapeyriere et al, 1993). However, since most of the FGF family members have broad target cell specificities, and are expressed by multiple cell types, it is unclear what role they may play in the induction of specific epithelial components by the mesenchyme.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pattern of keratinocyte growth factor and keratinocyte growth factor receptor expression during mouse fetal development suggests a role in mediating morphogenetic mesenchymal‐epithelial interactions

Finch

Cunha

Rubin

et al. 1995

Developmental Dynamics

266

172

View full text Add to dashboard Cite

Mesenchymal cells are required for the induction of epithelial development during mammalian organogenesis. Keratinocyte growth factor (KGF) is a mesenchymally derived mitogen with specific activity for epithelial cells, suggesting that it may play a role in mediating these interactions. To further evaluate this hypothesis, in situ hybridization was used to examine the spatial distribution of KGF and KGF receptor (KGFR) transcripts during organogenesis and limb formation in mouse embryos (days 14.5 through 16.5). To facilitate this aim, mouse KGF cDNA clones were isolated. There was extensive identity between the deduced mouse KGF protein sequence and that of its human and rat cognates, indicating that this gene has been highly conserved during mammalian evolution. In addition, mouse KGF protein was purified from fibroblasts and demonstrated to be structurally and functionally similar to human KGF protein. For organs within the integumental, respiratory, gastrointestinal, and urogenital systems, whose development is dependent upon mesenchymal‐epithelial interactions, KGF mRNA was detected in mesenchymal cells, while epithelial cells expressed transcripts for the KGFR. KGF and KGFR mRNA was also expressed in certain other tissues such as perichondrium, cartilage of developing bones, developing skeletal muscle, and visceral smooth muscle whose development is not regulated by mesenchymal‐epithelial interactions. KGF expression was also detected in tissues isolated from human embryos, suggesting similar functions for KGF in human development. Taken together, our results suggest that KGF plays an important role in mediating mesenchymal‐epithelial interactions during organogenesis, but may also have other developmental functions in tissues not governed by such interactions. ©1995 Wiley‐Liss, Inc.

show abstract

Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Pattern of keratinocyte growth factor and keratinocyte growth factor receptor expression during mouse fetal development suggests a role in mediating morphogenetic mesenchymal‐epithelial interactions

Finch

Cunha

Rubin

et al. 1995

Developmental Dynamics

266

172

View full text Add to dashboard Cite

show abstract

“…To date, 23 Fgf ligands (Fgf1-23) have been described in tetrapods (reviewed by Ornitz and Itoh, 2001) and several of these ligands are known to be expressed in early mesodermal progenitors in mice, including Fgf3 (Wilkinson et al, 1988), Fgf4 (Niswander and Martin, 1992;Drucker and Goldfarb, 1993), Fgf5 (Haub and Goldfarb, 1991;Hébert et al, 1991) and Fgf8 (Heikinheimo et al, 1994;Ohuchi et al, 1994;Crossley and Martin, 1995). Mutational analyses in mice, however, suggest that not all of these ligands are required for the development of mesoderm.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish fgf24functions withfgf8to promote posterior mesodermal development

2003

View full text Add to dashboard Cite

Fibroblast growth factor (Fgf) signaling plays an important role during development of posterior mesoderm in vertebrate embryos. Blocking Fgf signaling by expressing a dominant-negative Fgf receptor inhibits posterior mesoderm development. In mice, Fgf8 appears to be the principal ligand required for mesodermal development, as mouse Fgf8 mutants do not form mesoderm. In zebrafish, Fgf8 is encoded by the acerebellarlocus, and, similar to its mouse otholog, is expressed in early mesodermal precursors during gastrulation. However, zebrafish fgf8 mutants have only mild defects in posterior mesodermal development, suggesting that it is not the only Fgf ligand involved in the development of this tissue. We report here the identification of an fgf8-related gene in zebrafish, fgf24, that is co-expressed with fgf8 in mesodermal precursors during gastrulation. Using morpholino-based gene inactivation, we have analyzed the function of fgf24 during development. We found that inhibiting fgf24 function alone has no affect on the formation of posterior mesoderm. Conversely, inhibiting fgf24 function in embryos mutant for fgf8 blocks the formation of most posterior mesoderm. Thus, fgf8 and fgf24 are together required to promote posterior mesodermal development. We provide both phenotypic and genetic evidence that these Fgf signaling components interact with no tailand spadetail, two zebrafish T-box transcription factors that are required for the development of all posterior mesoderm. Last, we show that fgf24 is expressed in early fin bud mesenchyme and that inhibiting fgf24 function results in viable fish that lack pectoral fins.

show abstract

“…The migration of RNA molecular weight standards (Promega), in Kb, is indicated. Niswander and Martin, 1992;Haub and Goldfarb, 1991;Drucker and Goldfarb, 1993;deLapeyere et al, 1993), IGF RNA and proteins (Ralphs et al, 1990;Stylianopoulou et al, 1988), and TGFP4 (Jakowlew et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…However, to postulate a physiological role for a growth factor active on a given cell type in vitro, it is necessary to demonstrate its presence, when and where proliferation occurs in vivo. Many of the FGF isoforms have been shown to localize to areas where myoblast proliferation occurs during embryonic development (JosephSilverstein, 1989;Niswander and Martin, 1992;Haub and Goldfarb, 1991;Drucker and Goldfarb, 1993;deLapeyere et al, 1993). In the case of ACTH, we previously reported that ACTH-immunoreactive peptides are present in post-implantation mouse embryo, mainly localized to neural and muscle anlagens .…”

Section: Introductionmentioning

confidence: 99%

Pro‐opiomelanocortin gene is expressed in post‐implantation mouse embryos and enhances growth potential of myogenic cells

Angelis

Monaco

et al. 1993

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

Murine FGF-4 gene expression is spatially restricted within embryonic skeletal muscle and other tissues

Cited by 50 publications

References 27 publications

Pattern of keratinocyte growth factor and keratinocyte growth factor receptor expression during mouse fetal development suggests a role in mediating morphogenetic mesenchymal‐epithelial interactions

Pattern of keratinocyte growth factor and keratinocyte growth factor receptor expression during mouse fetal development suggests a role in mediating morphogenetic mesenchymal‐epithelial interactions

Zebrafish fgf24functions withfgf8to promote posterior mesodermal development

Pro‐opiomelanocortin gene is expressed in post‐implantation mouse embryos and enhances growth potential of myogenic cells

Contact Info

Product

Resources

About