Murine <i>vs</i> human tissue compositions: implications of using human tissue compositions for photon energy absorption in mice

Schyns, Lotte E J R; Eekers, Daniëlle; Heyden, Brent van der; Almeida, Isabel P.; Vaniqui, Ana; Verhaegen, Frank

doi:10.1259/bjr.20180454

Cited by 11 publications

(10 citation statements)

References 28 publications

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“…Furthermore, the dose to other organs at risk in the irradiation field such as the heart influence pulmonary function and indirectly contribute to adverse effects and should be appropriately modeled as well (198). In this respect dose calculations on human tissues irradiation studies with MeV beams cannot be directly translated to mouse preclinical irradiation studies using KeV beams (199). The use of preclinical micro-irradiators with integrated CT imaging are a new tool to evaluate radiation-induced toxicity closer to clinical irradiation set-up.…”

Section: Intervention and Preventionmentioning

confidence: 99%

Radiation-Induced Lung Injury (RILI)

et al. 2019

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Radiation pneumonitis (RP) and radiation fibrosis (RF) are two dose-limiting toxicities of radiotherapy (RT), especially for lung, and esophageal cancer. It occurs in 5–20% of patients and limits the maximum dose that can be delivered, reducing tumor control probability (TCP) and may lead to dyspnea, lung fibrosis, and impaired quality of life. Both physical and biological factors determine the normal tissue complication probability (NTCP) by Radiotherapy. A better understanding of the pathophysiological sequence of radiation-induced lung injury (RILI) and the intrinsic, environmental and treatment-related factors may aid in the prevention, and better management of radiation-induced lung damage. In this review, we summarize our current understanding of the pathological and molecular consequences of lung exposure to ionizing radiation, and pharmaceutical interventions that may be beneficial in the prevention or curtailment of RILI, and therefore enable a more durable therapeutic tumor response.

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Section: Intervention and Preventionmentioning

confidence: 99%

Radiation-Induced Lung Injury (RILI)

et al. 2019

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“…When comparing to the ICRU-44 bone-equivalent plastic (ICRU 1989), the linear attenuation coefficient of our mixture is systematically lower over the whole energy range. Even though it was demonstrated that murine tissue differs, in composition and density, from that of humans (Schyns et al 2019), it is a common practice to use human tissue composition for dose calculation in mice. We can therefore conclude that this surrogate might be used also for lower irradiation energies.…”

Section: Discussionmentioning

confidence: 99%

Development and characterization of modular mouse phantoms for end-to-end testing and training in radiobiology experiments

Wegner

Frenzel²,

Krause³

et al. 2023

Phys. Med. Biol.

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Objective. In radiation oncology, experiments are often carried out using mice as a model for in-vivo research studies. Due to recent technological advances in the development of high-precision small-animal irradiation facilities, the importance of quality assurance for both dosimetry and imaging is increasing. Additive manufacturing (AM) offers the possibility to produce complex models from a three-dimensional data set and to build cost-effective phantoms that can easily be adapted to different purposes. The aim of this work was therefore to develop detailed anatomical mouse models for quality assurance and end-to-end testing of small-animal irradiation and imaging by means of AM. Approach. Two mouse phantom concepts were designed, constructed, and examined for this purpose. The first model includes cavities corresponding to the most important organs. The final solid model was constructed using AM in two separate parts that can be attached with a plug connection after filling these cavities with tissue-equivalent mixtures. Moreover, different radiation dosimeters can be placed in the lower part of the model. For the second concept, AM was used for building modules like the phantom outer shell and bones, so that different mixtures can be used as a filling, without modifying the phantom structure. Main results. CT as well as Micro-CT scans of both concepts showed an excellent quality and adequate image contrast, with material attenuation properties close to those of mouse tissues, apart from the current bone surrogates. Radiation dose measurements with radiochromic films were, with some exceptions in areas with larges bone volumes, in agreement with calculations within less than ± 4 %. Significance. AM shows great potential for the development of mouse models that are inexpensive, easy to adapt, and accurate, thus enabling their use for quality assurance in small-animal radiotherapy and imaging. The introduction of such 3D-printable mouse phantoms in the workflow could also significantly reduce the use of living animals for optimization and testing of new imaging and irradiation protocols.

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“…Here, a randomly sampled degree of variation is included in the simulation of the X-ray projections. The variation is included in the definition of the random anisotropic voxel size [0.17–0.29 mm], the three-dimensional digital phantom position [± 10 mm], the X-ray projection angle [0–359 degrees], the elemental variation 16 in the tissue composition of ± 5%, and finally a mass density variation of ± 5%. Furthermore, the dataset was augmented through the simulation of mathematical cylinders varying in diameter, position, orientation, and tissue composition.…”

Section: Methodsmentioning

confidence: 99%

“…A hardened X-ray spectrum becomes more penetrating and is therefore introducing an error in the beam intensity measurements. A CBCT image reconstruction without beam hardening correction yields a degraded image quality across uniform volumes, expressed as non-uniform CT numbers, which negatively affects the dosimetric quantities 16 , but also the accuracy of CBCT image feature extraction for which the radiation cabinets could potentially be used 4 , 17 .…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have already indicated that tissue identification is a source of errors in preclinical kV dose calculations due to the strong dependence of the photo-electron absorption cross sections on the effective atomic number of the tissues 16 , 18 . Therefore, beam hardening correction algorithms and alternative solutions have been suggested in literature to obtain a desirable CT image quality.…”

Section: Introductionmentioning

confidence: 99%

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Virtual monoenergetic micro-CT imaging in mice with artificial intelligence

Heyden

Roden

Dok

et al. 2022

Sci Rep

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Micro cone-beam computed tomography (µCBCT) imaging is of utmost importance for carrying out extensive preclinical research in rodents. The imaging of animals is an essential step prior to preclinical precision irradiation, but also in the longitudinal assessment of treatment outcomes. However, imaging artifacts such as beam hardening will occur due to the low energetic nature of the X-ray imaging beam (i.e., 60 kVp). Beam hardening artifacts are especially difficult to resolve in a ‘pancake’ imaging geometry with stationary source and detector, where the animal is rotated around its sagittal axis, and the X-ray imaging beam crosses a wide range of thicknesses. In this study, a seven-layer U-Net based network architecture (vMonoCT) is adopted to predict virtual monoenergetic X-ray projections from polyenergetic X-ray projections. A Monte Carlo simulation model is developed to compose a training dataset of 1890 projection pairs. Here, a series of digital anthropomorphic mouse phantoms was derived from the reference DigiMouse phantom as simulation geometry. vMonoCT was trained on 1512 projection pairs (= 80%) and tested on 378 projection pairs (= 20%). The percentage error calculated for the test dataset was 1.7 ± 0.4%. Additionally, the vMonoCT model was evaluated on a retrospective projection dataset of five mice and one frozen cadaver. It was found that beam hardening artifacts were minimized after image reconstruction of the vMonoCT-corrected projections, and that anatomically incorrect gradient errors were corrected in the cranium up to 15%. Our results disclose the potential of Artificial Intelligence to enhance the µCBCT image quality in biomedical applications. vMonoCT is expected to contribute to the reproducibility of quantitative preclinical applications such as precision irradiations in X-ray cabinets, and to the evaluation of longitudinal imaging data in extensive preclinical studies.

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Murine vs human tissue compositions: implications of using human tissue compositions for photon energy absorption in mice

Cited by 11 publications

References 28 publications

Radiation-Induced Lung Injury (RILI)

Radiation-Induced Lung Injury (RILI)

Development and characterization of modular mouse phantoms for end-to-end testing and training in radiobiology experiments

Virtual monoenergetic micro-CT imaging in mice with artificial intelligence

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