Murine interferon-gamma inducible protein-10 (IP-10) secreted by Lactococcus lactis chemo-attracts human CD3+ lymphocytes

Villatoro-Hernandez, Julio; Arce-Mendoza, Alma Y.; Rosas-Taraco, Adrián G.; Esparza‐González, Sandra Cecilia; Guzmán-López, Santos; Elizondo‐Omaña, Rodrigo Enrique; Chávez‐Reyes, Arturo; Saucedo‐Cárdenas, Odila; Luna, Roberto Montes de Oca

doi:10.1007/s10529-009-0081-7

Cited by 3 publications

References 17 publications

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Heterologous Protein Expression by Lactococcus lactis

Villatoro-Hernandez

Kuipers

Saucedo‐Cárdenas

et al. 2011

Recombinant Gene Expression

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This chapter describes the use of Lactococcus lactis as a safe and efficient cell factory to produce heterologous proteins of medical interest. The relevance of the use of this lactic acid bacterium (LAB) is that it is a noncolonizing, nonpathogenic microorganism that can be delivered in vivo at a mucosal level. The use of strains of L. lactis in clinical trials in humans to alleviate inflammatory bowel diseases has opened up the possibility of using this same LAB to target other diseases.Several crucial aspects are addressed in this chapter, such as the expression of heterologous protein, subcellular compartment into which the heterologous protein is located, and description of a standardized protocol to process samples in cell and cell-free fractions to detect the targeted protein expressed by L. lactis.

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Heterologous Protein Expression by Lactococcus lactis

Villatoro-Hernandez

Kuipers

Saucedo‐Cárdenas

et al. 2011

Recombinant Gene Expression

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Lactobacillus plantarum displaying CCL3 chemokine in fusion with HIV-1 Gag derived antigen causes increased recruitment of T cells

et al. 2015

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BackgroundChemokines are attractive candidates for vaccine adjuvants due to their ability to recruit the immune cells. Lactic acid bacteria (LAB)-based delivery vehicles have potential to be used as a cheap and safe option for vaccination. Chemokine produced on the surface of LAB may potentially enhance the immune response to an antigen and this approach can be considered in development of future mucosal vaccines.ResultsWe have constructed strains of Lactobacillusplantarum displaying a chemokine on their surface. L. plantarum was genetically engineered to express and anchor to the surface a protein called CCL3Gag. CCL3Gag is a fusion protein comprising of truncated HIV-1 Gag antigen and the murine chemokine CCL3, also known as MIP-1α. Various surface anchoring strategies were explored: (1) a lipobox-based covalent membrane anchor, (2) sortase-mediated covalent cell wall anchoring, (3) LysM-based non-covalent cell wall anchoring, and (4) an N-terminal signal peptide-based transmembrane anchor. Protein production and correct localization were confirmed using Western blotting, flow cytometry and immunofluorescence microscopy. Using a chemotaxis assay, we demonstrated that CCL3Gag-producing L. plantarum strains are able to recruit immune cells in vitro.ConclusionsThe results show the ability of engineered L. plantarum to produce a functional chemotactic protein immobilized on the bacterial surface. We observed that the activity of surface-displayed CCL3Gag differed depending on the type of anchor used. The chemokine which is a part of the bacteria-based vaccine may increase the recruitment of immune cells and, thereby, enhance the reaction of the immune system to the vaccine.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-015-0360-z) contains supplementary material, which is available to authorized users.

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Chemokine-Based Immunotherapy: Delivery Systems and Combination Therapies

Mohit

Rafati

2012

Immunotherapy

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A major role of chemokines is to mediate leukocyte migration through interaction with G-protein-coupled receptors. Various delivery systems have been developed to utilize the chemokine properties for combating disease. Viral and mutant viral vectors expressing chemokines, genetically modified dendritic cells with chemokine or chemokine receptors, engineered chemokine-expressing tumor cells and pDNA encoding chemokines are among these methods. Another approach for inducing a targeted immune response is fusion of a targeting antibody or antibody fragment to a chemokine. In addition, chemokines induce more effective antitumor immunity when used as adjuvants. In this regard, chemokines are codelivered along with antigens or fused as a targeting unit with antigenic moieties. In this review, several chemokines with their role in inducing immune response against different diseases are discussed, with a major emphasis on cancer.

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Murine interferon-gamma inducible protein-10 (IP-10) secreted by Lactococcus lactis chemo-attracts human CD3+ lymphocytes

Cited by 3 publications

References 17 publications

Heterologous Protein Expression by Lactococcus lactis

Heterologous Protein Expression by Lactococcus lactis

Lactobacillus plantarum displaying CCL3 chemokine in fusion with HIV-1 Gag derived antigen causes increased recruitment of T cells

Chemokine-Based Immunotherapy: Delivery Systems and Combination Therapies

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