Murine Ly-6 multigene family is located on chromosome 15.

LeClair, Kenneth; Rabin, Mark; Nesbitt, Muriel N.; Pravtcheva, Dimitrina D.; Ruddle, Frank H.; Palfree, Roger G. E.; Bothwell, Alfred L.M.

doi:10.1073/pnas.84.6.1638

Cited by 69 publications

(27 citation statements)

References 32 publications

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“…Genes for the Ly-6 proteins in mice are found on chromosome 15 (28). Many Ly-6 molecules, including Ly-6C and Ly-6G, possess conserved domains containing cysteine residues.…”

Section: Discussionmentioning

confidence: 99%

Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

Nagendra

Schlueter

2004

Cytometry Pt A

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BackgroundThe Ly‐6 family has many members, including Ly‐6C and Ly‐6G. A previous study suggested that the anti‐Ly‐6G antibody, RB6‐8C5, may react with Ly‐6Chi murine bone marrow (BM) cells. This finding has been interpreted as cross‐reactivity of RB6‐8C5 with the Ly‐6C antigen, and has been generalized to many hematopoietic cell types, using the terminology Ly‐6G/C. The present study was undertaken to determine whether anti‐Ly‐6G antibodies truly cross‐react with the Ly‐6C antigen on multiple hematopoietic cell types.MethodsSplenocytes, thymocytes, and BM cells obtained from Ly‐6.1 and Ly‐6.2 strains of mice were stained with a variety of antibodies to Ly‐6C and Ly‐6G. Flow cytometric analysis was performed on these populations.ResultsEvaluation of anti‐Ly‐6C and anti‐Ly‐6G staining showed only Ly‐6C expression and no Ly‐6G expression on subsets of splenic T and B cells and thymocytes from Ly‐6.1 and Ly‐6.2 mice. Bone marrow cells were identified that express both Ly‐6G and Ly‐6C; no Ly‐6G+Ly‐6C− populations were seen.ConclusionsMultiple Ly‐6C+ hematopoietic cell populations were identified that do not stain with anti‐Ly‐6G antibodies. This calls into question the use of the Ly‐6G/C nomenclature and suggests that epitopes recognized by anti‐Ly‐6G antibodies should simply be designated Ly‐6G. © 2004 Wiley‐Liss, Inc.

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“…Genes for the Ly-6 proteins in mice are found on chromosome 15 (28). Many Ly-6 molecules, including Ly-6C and Ly-6G, possess conserved domains containing cysteine residues.…”

Section: Discussionmentioning

confidence: 99%

Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

Nagendra

Schlueter

2004

Cytometry Pt A

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“…L y6 is a multigene family of GPI-anchored cell surface glycoproteins mainly expressed on cells of hemopoietic origin (1). This family consists of several members that are mainly involved in cellular interactions and T cell activation.…”

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confidence: 99%

Ly6C Induces Clustering of LFA-1 (CD11a/CD18) and Is Involved in Subtype-Specific Adhesion of CD8 T Cells

Jaakkola

Merinen

Jalkanen

et al. 2003

The Journal of Immunology

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Ly6C is a hemopoietic cell differentiation Ag found on a subset of CD8 T cells in the periphery. It is involved in target cell killing by CTLs, augments TCR-mediated activation of IL-2 and IFN-γ production in CD8 T cells, and regulates CD8 T cell homing in vivo. In this study, we show that cross-linking of Ly6C causes clustering of LFA-1 (CD11a/CD18) on the surface of CD8 T cells via a mechanism dependent on reorganization of actin cytoskeleton and intracellular protease, calpain, but not the phosphatidylinositol 3-kinase pathway. In the capillary flow-adhesion assay, Ly6C cross-linking significantly augments lymphocyte adhesion to endothelium, and this is inhibited by an Ab that blocks LFA-1 function. Furthermore, upon in vitro cross-linking and during in vivo homing into lymph nodes, Ly6C is transiently lost from cell surface but becomes re-expressed on lymph node-resident CD8 T cells. The abilities of Ly6C to induce LFA-1 clustering and to be re-expressed after signaling-associated down-regulation may be important in regulating the homing of CD8 T cells into lymph nodes and in subsequent steps of CD8 T cell activation and effector function that again involve LFA-1.

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“…L y-6 is a multigene family on chromosome 15 in mice (1). Proteins encoded by these genes are 10 -12 kDa and are anchored on the surface by a GPI moiety (2,3).…”

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confidence: 99%

A Monoclonal Antibody Against the 66-kDa Protein Expressed in Mouse Spleen and Thymus Inhibits Ly-6A.2-Dependent Cell-Cell Adhesion

English

Kosoy

Pawlinski

et al. 2000

The Journal of Immunology

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The Ly-6 locus encodes several cell surface proteins of 10–12 kDa. Some members of this multigene family may function in cell signaling and/or cell adhesion processes. T lymphocytes overexpressing Ly-6A.2 (one member of the Ly-6 gene family) protein homotypically aggregate when cultured in vitro. Further analysis of this homotypic aggregation suggests that Ly-6A.2 participates in cell-cell adhesion. These observations indicated the presence of a Ly-6 ligand(s) on the surface of lymphoid cells. In this study we report generation of a hamster mAb, 9AB2, that blocks Ly-6A.2-dependent cell-cell adhesion. The 9AB2 Ab recognizes a 66-kDa glycoprotein with unique tissue expression. The 9AB2 mAb does not bind Ly-6A.2, but coimmunoprecipitates Ly-6A.2 molecule. Moreover, 9AB2 Ag-expressing thymocytes specifically bind to Chinese hamster ovary cells overexpressing Ly-6A.2 protein, and this binding is specifically blocked by 9AB2 and anti-Ly-6A.2 Abs. These results suggest that the 66-kDa protein recognized by 9AB2 mAb is the putative ligand for Ly-6A.2.

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Murine Ly-6 multigene family is located on chromosome 15.

Cited by 69 publications

References 32 publications

Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

Ly6C Induces Clustering of LFA-1 (CD11a/CD18) and Is Involved in Subtype-Specific Adhesion of CD8 T Cells

A Monoclonal Antibody Against the 66-kDa Protein Expressed in Mouse Spleen and Thymus Inhibits Ly-6A.2-Dependent Cell-Cell Adhesion

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