Murine mast cells attach to and migrate on laminin‐, fibronectin‐, and matrigel‐coated surfaces in response to Fc_εRI‐mediated signals

Abstract

We have examined the possibility that mouse bone marrow-derived cultured mast cells (BMCMC) have the capacity to attach to and migrate on extracellular matrix components in vitro through the use of time lapse videography. Unactivated mast cells did not display significant interaction with slide flasks coated with either 3% BSA or collagen IV, and Fc epsilon RI-mediated activation of BMCMC did not appreciably increase their attachment and migratory characteristics. Both activated and unactivated BMCMC adhered to surfaces coated with a synthetic IKVAV laminin polypeptide, but this association resulted in the immobilization of the cells to the substrate. BMCMC did not adhere to surfaces coated with laminin, fibronectin or matrigel until Fc epsilon RI-mediated activation, after which they displayed rapid, random movement on these surfaces. Cells continually interacted with laminin, fibronectin or matrigel by flattening, interspaced by periods of movement as rounded cells with small pseudopodia. The mean velocity of BMCMC on laminin, fibronectin or matrigel was similar and averaged approximately 180 microns/hr. The mean velocity of BMCMC on these three substrates was not significantly different from the mean velocity of monocytes on laminin. The movement of BMCMC on these substrates demonstrated a directional tendency. In summary, these results demonstrate that mast cells activated through Fc epsilon RI are capable of attachment to and motion on components of extracellular matrix, and demonstrate one mechanism by which mast cells may migrate to areas of inflammation and wound repair.

Pak2 Kinase Restrains Mast Cell FcϵRI Receptor Signaling through Modulation of Rho Protein Guanine Nucleotide Exchange Factor (GEF) Activity

“…Mast cell activation of Fc⑀RI receptor stimulates an increase in cell adhesion, and this adherence is required to facilitate localized synthesis of cytokines (19,20). Given the role of Paks in cell migration, adhesion, and cytoskeleton regulation, we sought to discover a role for Paks in mast cell adhesion.…”

Fer Kinase Is Required for Sustained p38 Kinase Activation and Maximal Chemotaxis of Activated Mast Cells

“…Mast cells also display increased motility upon activation of FcεRI and Kit signaling (27,54,64). Since we observed reduced p38 activation in fer DR/DR BMMCs (Fig.…”

“…However, the aggregation of FcεRI is required for degranulation and release of lipid mediators. Mast cell activation downstream of either FcεRI or the Kit receptor also leads to increased cell adhesion (13,53) and increased cell motility (54,64). The activation of p38 MAP kinase is critical for both antigen-dependent mast cell migration via FcεRI (27) and chemotaxis upon Kit receptor activation (59).…”

Regulatory effects of stem cell factor and interleukin-4 on adhesion of human mast cells to extracellular matrix proteins

“…It was reported that bone marrow-derived mouse mast cells adhere to laminin and fibronectin and that this adherence is induced by Fc⑀RI-mediated signals and SCF. 12,13 Studies in humans showed that mast cells from uterus and lung express the ␤1 integrins ␣4␤1 and ␣5␤1, known as receptors for fibronectin, and that skin mast cells adhere to fibronectin and laminin. [14][15][16] The immature human mast cell line (HMC)-1 also expresses ␣2␤1, ␣3␤1, ␣6␤1, ␣V␤1, ␣V␤5, and CD44.…”